tetano
Editor, Senior Moderator
Cell Rep
. 2022 Oct 18;41(3):111496.
doi: 10.1016/j.celrep.2022.111496.
SARS-CoV-2 infections elicit higher levels of original antigenic sin antibodies compared with SARS-CoV-2 mRNA vaccinations
Elizabeth M Anderson[SUP] 1 [/SUP], Shuk Hang Li[SUP] 1 [/SUP], Moses Awofolaju[SUP] 1 [/SUP], Theresa Eilola[SUP] 1 [/SUP], Eileen Goodwin[SUP] 1 [/SUP], Marcus J Bolton[SUP] 1 [/SUP], Sigrid Gouma[SUP] 1 [/SUP], Tomaz B Manzoni[SUP] 1 [/SUP], Philip Hicks[SUP] 1 [/SUP], Rishi R Goel[SUP] 2 [/SUP], Mark M Painter[SUP] 2 [/SUP], Sokratis A Apostolidis[SUP] 3 [/SUP], Divij Mathew[SUP] 2 [/SUP], Debora Dunbar[SUP] 4 [/SUP], Danielle Fiore[SUP] 5 [/SUP], Amanda Brock[SUP] 6 [/SUP], JoEllen Weaver[SUP] 6 [/SUP], John S Millar[SUP] 7 [/SUP], Stephanie DerOhannessian[SUP] 8 [/SUP], UPenn COVID Processing Unit; Allison R Greenplate[SUP] 9 [/SUP], Ian Frank[SUP] 4 [/SUP], Daniel J Rader[SUP] 8 [/SUP], E John Wherry[SUP] 10 [/SUP], Paul Bates[SUP] 1 [/SUP], Scott E Hensley[SUP] 11 [/SUP]
Affiliations
Abstract
It is important to determine if severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections and SARS-CoV-2 mRNA vaccinations elicit different types of antibodies. Here, we characterize the magnitude and specificity of SARS-CoV-2 spike-reactive antibodies from 10 acutely infected health care workers with no prior SARS-CoV-2 exposure history and 23 participants who received SARS-CoV-2 mRNA vaccines. We found that infection and primary mRNA vaccination elicit S1- and S2-reactive antibodies, while secondary vaccination boosts mostly S1 antibodies. Using absorption assays, we found that SARS-CoV-2 infections elicit a large proportion of original antigenic sin-like antibodies that bind efficiently to the spike of common seasonal human coronaviruses but poorly to the spike of SARS-CoV-2. In converse, vaccination modestly boosts antibodies reactive to the spike of common seasonal human coronaviruses, and these antibodies cross-react more efficiently to the spike of SARS-CoV-2. Our data indicate that SARS-CoV-2 infections and mRNA vaccinations elicit fundamentally different antibody responses.
Keywords: CP: Immunology; SARS-CoV-2; antibodies; coronavirus; mRNA vaccines; original antigenic sin.
. 2022 Oct 18;41(3):111496.
doi: 10.1016/j.celrep.2022.111496.
SARS-CoV-2 infections elicit higher levels of original antigenic sin antibodies compared with SARS-CoV-2 mRNA vaccinations
Elizabeth M Anderson[SUP] 1 [/SUP], Shuk Hang Li[SUP] 1 [/SUP], Moses Awofolaju[SUP] 1 [/SUP], Theresa Eilola[SUP] 1 [/SUP], Eileen Goodwin[SUP] 1 [/SUP], Marcus J Bolton[SUP] 1 [/SUP], Sigrid Gouma[SUP] 1 [/SUP], Tomaz B Manzoni[SUP] 1 [/SUP], Philip Hicks[SUP] 1 [/SUP], Rishi R Goel[SUP] 2 [/SUP], Mark M Painter[SUP] 2 [/SUP], Sokratis A Apostolidis[SUP] 3 [/SUP], Divij Mathew[SUP] 2 [/SUP], Debora Dunbar[SUP] 4 [/SUP], Danielle Fiore[SUP] 5 [/SUP], Amanda Brock[SUP] 6 [/SUP], JoEllen Weaver[SUP] 6 [/SUP], John S Millar[SUP] 7 [/SUP], Stephanie DerOhannessian[SUP] 8 [/SUP], UPenn COVID Processing Unit; Allison R Greenplate[SUP] 9 [/SUP], Ian Frank[SUP] 4 [/SUP], Daniel J Rader[SUP] 8 [/SUP], E John Wherry[SUP] 10 [/SUP], Paul Bates[SUP] 1 [/SUP], Scott E Hensley[SUP] 11 [/SUP]
Affiliations
- PMID: 36261003
- DOI: 10.1016/j.celrep.2022.111496
Abstract
It is important to determine if severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections and SARS-CoV-2 mRNA vaccinations elicit different types of antibodies. Here, we characterize the magnitude and specificity of SARS-CoV-2 spike-reactive antibodies from 10 acutely infected health care workers with no prior SARS-CoV-2 exposure history and 23 participants who received SARS-CoV-2 mRNA vaccines. We found that infection and primary mRNA vaccination elicit S1- and S2-reactive antibodies, while secondary vaccination boosts mostly S1 antibodies. Using absorption assays, we found that SARS-CoV-2 infections elicit a large proportion of original antigenic sin-like antibodies that bind efficiently to the spike of common seasonal human coronaviruses but poorly to the spike of SARS-CoV-2. In converse, vaccination modestly boosts antibodies reactive to the spike of common seasonal human coronaviruses, and these antibodies cross-react more efficiently to the spike of SARS-CoV-2. Our data indicate that SARS-CoV-2 infections and mRNA vaccinations elicit fundamentally different antibody responses.
Keywords: CP: Immunology; SARS-CoV-2; antibodies; coronavirus; mRNA vaccines; original antigenic sin.