tetano
Editor, Senior Moderator
Cell Rep Med
. 2022 Nov 15;100845.
doi: 10.1016/j.xcrm.2022.100845. Online ahead of print.
Tfh cells and the germinal center are required for memory B cell formation & humoral immunity after ChAdOx1 nCoV-19 vaccination
William S Foster[SUP] 1 [/SUP], Jia Le Lee[SUP] 1 [/SUP], Nazia Thakur[SUP] 2 [/SUP], Joseph Newman[SUP] 3 [/SUP], Alexandra J Spencer[SUP] 4 [/SUP], Sophie Davies[SUP] 5 [/SUP], Danielle Woods[SUP] 4 [/SUP], Leila Godfrey[SUP] 4 [/SUP], Iain M Hay[SUP] 6 [/SUP], Silvia Innocentin[SUP] 1 [/SUP], Juan Carlos Yam-Puc[SUP] 7 [/SUP], Emily C Horner[SUP] 7 [/SUP], Hayley J Sharpe[SUP] 8 [/SUP], James E Thaventhiran[SUP] 7 [/SUP], Dalan Bailey[SUP] 3 [/SUP], Teresa Lambe[SUP] 9 [/SUP], Michelle A Linterman[SUP] 10 [/SUP]
Affiliations
Abstract
Emergence from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has been facilitated by the rollout of effective vaccines. Successful vaccines generate high-affinity plasma blasts and long-lived protective memory B cells. Here, we show a requirement for T follicular helper (Tfh) cells and the germinal center reaction for optimal serum antibody and memory B cell formation after ChAdOx1 nCoV-19 vaccination. We found that Tfh cells play an important role in expanding antigen-specific B cells while identifying Tfh-cell-dependent and -independent memory B cell subsets. Upon secondary vaccination, germinal center B cells generated during primary immunizations can be recalled as germinal center B cells again. Likewise, primary immunization GC-Tfh cells can be recalled as either Tfh or Th1 cells, highlighting the pluripotent nature of Tfh cell memory. This study demonstrates that ChAdOx1 nCoV-19-induced germinal centers are a critical source of humoral immunity.
Keywords: CD40L; ChAdOx1 nCoV-19; SARS-CoV-2; Tfh cells; germinal center; vaccine.
. 2022 Nov 15;100845.
doi: 10.1016/j.xcrm.2022.100845. Online ahead of print.
Tfh cells and the germinal center are required for memory B cell formation & humoral immunity after ChAdOx1 nCoV-19 vaccination
William S Foster[SUP] 1 [/SUP], Jia Le Lee[SUP] 1 [/SUP], Nazia Thakur[SUP] 2 [/SUP], Joseph Newman[SUP] 3 [/SUP], Alexandra J Spencer[SUP] 4 [/SUP], Sophie Davies[SUP] 5 [/SUP], Danielle Woods[SUP] 4 [/SUP], Leila Godfrey[SUP] 4 [/SUP], Iain M Hay[SUP] 6 [/SUP], Silvia Innocentin[SUP] 1 [/SUP], Juan Carlos Yam-Puc[SUP] 7 [/SUP], Emily C Horner[SUP] 7 [/SUP], Hayley J Sharpe[SUP] 8 [/SUP], James E Thaventhiran[SUP] 7 [/SUP], Dalan Bailey[SUP] 3 [/SUP], Teresa Lambe[SUP] 9 [/SUP], Michelle A Linterman[SUP] 10 [/SUP]
Affiliations
- PMID: 36455555
- DOI: 10.1016/j.xcrm.2022.100845
Abstract
Emergence from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has been facilitated by the rollout of effective vaccines. Successful vaccines generate high-affinity plasma blasts and long-lived protective memory B cells. Here, we show a requirement for T follicular helper (Tfh) cells and the germinal center reaction for optimal serum antibody and memory B cell formation after ChAdOx1 nCoV-19 vaccination. We found that Tfh cells play an important role in expanding antigen-specific B cells while identifying Tfh-cell-dependent and -independent memory B cell subsets. Upon secondary vaccination, germinal center B cells generated during primary immunizations can be recalled as germinal center B cells again. Likewise, primary immunization GC-Tfh cells can be recalled as either Tfh or Th1 cells, highlighting the pluripotent nature of Tfh cell memory. This study demonstrates that ChAdOx1 nCoV-19-induced germinal centers are a critical source of humoral immunity.
Keywords: CD40L; ChAdOx1 nCoV-19; SARS-CoV-2; Tfh cells; germinal center; vaccine.