tetano
Editor, Senior Moderator
Cell Rep Med
. 2021 Sep 28;100420.
doi: 10.1016/j.xcrm.2021.100420. Online ahead of print.
Intradermal-delivered DNA vaccine induces durable immunity mediating a reduction in viral load in a rhesus macaque SARS-CoV-2 challenge model
Ami Patel[SUP] 1 [/SUP], Jewell N Walters[SUP] 2 [/SUP], Emma L Reuschel[SUP] 1 [/SUP], Katherine Schultheis[SUP] 2 [/SUP], Elizabeth Parzych[SUP] 1 [/SUP], Ebony N Gary[SUP] 1 [/SUP], Igor Maricic[SUP] 2 [/SUP], Mansi Purwar[SUP] 1 [/SUP], Zeena Eblimit[SUP] 2 [/SUP], Susanne N Walker[SUP] 1 [/SUP], Diana Guimet[SUP] 2 [/SUP], Pratik Bhojnagarwala[SUP] 1 [/SUP], Opeyemi S Adeniji[SUP] 1 [/SUP], Arthur Doan[SUP] 2 [/SUP], Ziyang Xu[SUP] 1 [/SUP], Dustin Elwood[SUP] 2 [/SUP], Sophia M Reeder[SUP] 1 [/SUP], Laurent Pessaint[SUP] 3 [/SUP], Kevin Y Kim[SUP] 1 [/SUP], Anthony Cook[SUP] 3 [/SUP], Neethu Chokkalingam[SUP] 1 [/SUP], Brad Finneyfrock[SUP] 3 [/SUP], Edgar Tello-Ruiz[SUP] 1 [/SUP], Alan Dodson[SUP] 3 [/SUP], Jihae Choi[SUP] 1 [/SUP], Alison Generotti[SUP] 2 [/SUP], John Harrison[SUP] 3 [/SUP], Nicholas J Tursi[SUP] 1 [/SUP], Viviane M Andrade[SUP] 2 [/SUP], Yaya Dia[SUP] 1 [/SUP], Faraz I Zaidi[SUP] 1 [/SUP], Hanne Andersen[SUP] 3 [/SUP], Mohamed Abdel-Mohsen[SUP] 1 [/SUP], Mark G Lewis[SUP] 3 [/SUP], Kar Muthumani[SUP] 1 [/SUP], J Joseph Kim[SUP] 2 [/SUP], Daniel W Kulp[SUP] 1 [/SUP], Laurent M Humeau[SUP] 2 [/SUP], Stephanie J Ramos[SUP] 2 [/SUP], Trevor R F Smith[SUP] 2 [/SUP], David B Weiner[SUP] 1 [/SUP], Kate E Broderick[SUP] 2 [/SUP]
Affiliations
Abstract
Coronavirus disease 2019 (COVID-19), caused by the SARS-CoV-2 virus, has had a dramatic global impact on public health, social, and economic infrastructures. Here, we assess the immunogenicity and anamnestic protective efficacy in rhesus macaques of an intradermal (ID)-delivered SARS-CoV-2 spike DNA vaccine, INO-4800, currently being evaluated in clinical trials. Vaccination with INO-4800 induced T cell responses and induced spike antigen and RBD binding antibodies with ADCP and ADCD activity. Sera from the animals neutralized both the D614 and G614 SARS-CoV-2 pseudotype viruses. Several months after vaccination, animals were challenged with SARS-CoV-2 resulting in rapid recall of anti-SARS-CoV-2 spike protein T cell and neutralizing antibody responses. These responses were associated with lower viral loads in the lung. These studies support the immune impact of INO-4800 for inducing both humoral and cellular arms of the adaptive immune system which are likely important for providing durable protection against COVID-19 disease.
Keywords: COVID-19; ID DNA vaccine; SARS-CoV-2; challenge; coronavirus; electroporation; infectious disease; macaque; protection.
. 2021 Sep 28;100420.
doi: 10.1016/j.xcrm.2021.100420. Online ahead of print.
Intradermal-delivered DNA vaccine induces durable immunity mediating a reduction in viral load in a rhesus macaque SARS-CoV-2 challenge model
Ami Patel[SUP] 1 [/SUP], Jewell N Walters[SUP] 2 [/SUP], Emma L Reuschel[SUP] 1 [/SUP], Katherine Schultheis[SUP] 2 [/SUP], Elizabeth Parzych[SUP] 1 [/SUP], Ebony N Gary[SUP] 1 [/SUP], Igor Maricic[SUP] 2 [/SUP], Mansi Purwar[SUP] 1 [/SUP], Zeena Eblimit[SUP] 2 [/SUP], Susanne N Walker[SUP] 1 [/SUP], Diana Guimet[SUP] 2 [/SUP], Pratik Bhojnagarwala[SUP] 1 [/SUP], Opeyemi S Adeniji[SUP] 1 [/SUP], Arthur Doan[SUP] 2 [/SUP], Ziyang Xu[SUP] 1 [/SUP], Dustin Elwood[SUP] 2 [/SUP], Sophia M Reeder[SUP] 1 [/SUP], Laurent Pessaint[SUP] 3 [/SUP], Kevin Y Kim[SUP] 1 [/SUP], Anthony Cook[SUP] 3 [/SUP], Neethu Chokkalingam[SUP] 1 [/SUP], Brad Finneyfrock[SUP] 3 [/SUP], Edgar Tello-Ruiz[SUP] 1 [/SUP], Alan Dodson[SUP] 3 [/SUP], Jihae Choi[SUP] 1 [/SUP], Alison Generotti[SUP] 2 [/SUP], John Harrison[SUP] 3 [/SUP], Nicholas J Tursi[SUP] 1 [/SUP], Viviane M Andrade[SUP] 2 [/SUP], Yaya Dia[SUP] 1 [/SUP], Faraz I Zaidi[SUP] 1 [/SUP], Hanne Andersen[SUP] 3 [/SUP], Mohamed Abdel-Mohsen[SUP] 1 [/SUP], Mark G Lewis[SUP] 3 [/SUP], Kar Muthumani[SUP] 1 [/SUP], J Joseph Kim[SUP] 2 [/SUP], Daniel W Kulp[SUP] 1 [/SUP], Laurent M Humeau[SUP] 2 [/SUP], Stephanie J Ramos[SUP] 2 [/SUP], Trevor R F Smith[SUP] 2 [/SUP], David B Weiner[SUP] 1 [/SUP], Kate E Broderick[SUP] 2 [/SUP]
Affiliations
- PMID: 34604818
- PMCID: PMC8479327
- DOI: 10.1016/j.xcrm.2021.100420
Abstract
Coronavirus disease 2019 (COVID-19), caused by the SARS-CoV-2 virus, has had a dramatic global impact on public health, social, and economic infrastructures. Here, we assess the immunogenicity and anamnestic protective efficacy in rhesus macaques of an intradermal (ID)-delivered SARS-CoV-2 spike DNA vaccine, INO-4800, currently being evaluated in clinical trials. Vaccination with INO-4800 induced T cell responses and induced spike antigen and RBD binding antibodies with ADCP and ADCD activity. Sera from the animals neutralized both the D614 and G614 SARS-CoV-2 pseudotype viruses. Several months after vaccination, animals were challenged with SARS-CoV-2 resulting in rapid recall of anti-SARS-CoV-2 spike protein T cell and neutralizing antibody responses. These responses were associated with lower viral loads in the lung. These studies support the immune impact of INO-4800 for inducing both humoral and cellular arms of the adaptive immune system which are likely important for providing durable protection against COVID-19 disease.
Keywords: COVID-19; ID DNA vaccine; SARS-CoV-2; challenge; coronavirus; electroporation; infectious disease; macaque; protection.