tetano
Editor, Senior Moderator
Cell Rep Med
. 2022 Apr 22;100631.
doi: 10.1016/j.xcrm.2022.100631. Online ahead of print.
Distinct immune cell dynamics correlate with the immunogenicity and reactogenicity of SARS-CoV-2 mRNA vaccine
Tomohiro Takano[SUP] 1 [/SUP], Miwa Morikawa[SUP] 2 [/SUP], Yu Adachi[SUP] 1 [/SUP], Kiyomi Kabasawa[SUP] 2 [/SUP], Nicolas Sax[SUP] 3 [/SUP], Saya Moriyama[SUP] 1 [/SUP], Lin Sun[SUP] 1 [/SUP], Masanori Isogawa[SUP] 1 [/SUP], Ayae Nishiyama[SUP] 1 [/SUP], Taishi Onodera[SUP] 1 [/SUP], Kazutaka Terahara[SUP] 1 [/SUP], Keisuke Tonouchi[SUP] 1 [/SUP], Masashi Nishimura[SUP] 2 [/SUP], Kentaro Tomii[SUP] 4 [/SUP], Kazuo Yamashita[SUP] 3 [/SUP], Takayuki Matsumura[SUP] 5 [/SUP], Masaharu Shinkai[SUP] 6 [/SUP], Yoshimasa Takahashi[SUP] 7 [/SUP]
Affiliations
Abstract
Two doses of Pfizer/BioNTech BNT162b2 mRNA vaccine elicit robust severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-neutralizing antibodies with frequent adverse events. Here, by applying a high-dimensional immune profiling on 92 vaccinees, we identify six vaccine-induced immune dynamics that correlate with the amounts of neutralizing antibodies, the severity of adverse events, or both. The early dynamics of natural killer (NK)/monocyte subsets (CD16[SUP]+[/SUP] NK cells, CD56[SUP]high[/SUP] NK cells, and non-classical monocytes), dendritic cell (DC) subsets (DC3s and CD11c[SUP]-[/SUP] Axl[SUP]+[/SUP] Siglec-6[SUP]+[/SUP] [AS]-DCs), and NKT-like cells are revealed as the distinct cell correlates for neutralizing-antibody titers, severity of adverse events, and both, respectively. The cell correlates for neutralizing antibodies or adverse events are consistently associated with elevation of interferon gamma (IFN-γ)-inducible chemokines, but the chemokine receptors CCR2 and CXCR3 are expressed in distinct manners between the two correlates: vaccine-induced expression on the neutralizing-antibody correlate and constitutive expression on the adverse-event correlate. The finding may guide vaccine strategies that balance immunogenicity and reactogenicity.
Keywords: SARS-CoV-2; adverse events; cell dynamics; immune correlates; innate immunity; mRNA vaccine; neutralizing antibodies.
. 2022 Apr 22;100631.
doi: 10.1016/j.xcrm.2022.100631. Online ahead of print.
Distinct immune cell dynamics correlate with the immunogenicity and reactogenicity of SARS-CoV-2 mRNA vaccine
Tomohiro Takano[SUP] 1 [/SUP], Miwa Morikawa[SUP] 2 [/SUP], Yu Adachi[SUP] 1 [/SUP], Kiyomi Kabasawa[SUP] 2 [/SUP], Nicolas Sax[SUP] 3 [/SUP], Saya Moriyama[SUP] 1 [/SUP], Lin Sun[SUP] 1 [/SUP], Masanori Isogawa[SUP] 1 [/SUP], Ayae Nishiyama[SUP] 1 [/SUP], Taishi Onodera[SUP] 1 [/SUP], Kazutaka Terahara[SUP] 1 [/SUP], Keisuke Tonouchi[SUP] 1 [/SUP], Masashi Nishimura[SUP] 2 [/SUP], Kentaro Tomii[SUP] 4 [/SUP], Kazuo Yamashita[SUP] 3 [/SUP], Takayuki Matsumura[SUP] 5 [/SUP], Masaharu Shinkai[SUP] 6 [/SUP], Yoshimasa Takahashi[SUP] 7 [/SUP]
Affiliations
- PMID: 35545084
- DOI: 10.1016/j.xcrm.2022.100631
Abstract
Two doses of Pfizer/BioNTech BNT162b2 mRNA vaccine elicit robust severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-neutralizing antibodies with frequent adverse events. Here, by applying a high-dimensional immune profiling on 92 vaccinees, we identify six vaccine-induced immune dynamics that correlate with the amounts of neutralizing antibodies, the severity of adverse events, or both. The early dynamics of natural killer (NK)/monocyte subsets (CD16[SUP]+[/SUP] NK cells, CD56[SUP]high[/SUP] NK cells, and non-classical monocytes), dendritic cell (DC) subsets (DC3s and CD11c[SUP]-[/SUP] Axl[SUP]+[/SUP] Siglec-6[SUP]+[/SUP] [AS]-DCs), and NKT-like cells are revealed as the distinct cell correlates for neutralizing-antibody titers, severity of adverse events, and both, respectively. The cell correlates for neutralizing antibodies or adverse events are consistently associated with elevation of interferon gamma (IFN-γ)-inducible chemokines, but the chemokine receptors CCR2 and CXCR3 are expressed in distinct manners between the two correlates: vaccine-induced expression on the neutralizing-antibody correlate and constitutive expression on the adverse-event correlate. The finding may guide vaccine strategies that balance immunogenicity and reactogenicity.
Keywords: SARS-CoV-2; adverse events; cell dynamics; immune correlates; innate immunity; mRNA vaccine; neutralizing antibodies.