tetano
Editor, Senior Moderator
Cell Rep
. 2023 Jul 7;42(7):112766.
doi: 10.1016/j.celrep.2023.112766. Online ahead of print. Boost immunizations with NA-derived peptide conjugates achieve induction of NA inhibition antibodies and heterologous influenza protections
De-Jian Liu[SUP] 1 [/SUP], Cui-Cui Liu[SUP] 1 [/SUP], Xiu-Qin Zhong[SUP] 1 [/SUP], Xuan Wu[SUP] 1 [/SUP], Hui-Hui Zhang[SUP] 2 [/SUP], Shang-Wen Lu[SUP] 2 [/SUP], Zhuo-Ling Shen[SUP] 1 [/SUP], Wen-Wen Song[SUP] 1 [/SUP], Shi-Long Zhao[SUP] 1 [/SUP], You-Song Peng[SUP] 3 [/SUP], He-Ping Zheng[SUP] 3 [/SUP], Mu-Yang Wan[SUP] 1 [/SUP], Yao-Qing Chen[SUP] 4 [/SUP], Lei Deng[SUP] 5 [/SUP]
Affiliations
Neuraminidase is suggested as an important component for developing a universal influenza vaccine. Targeted induction of neuraminidase-specific broadly protective antibodies by vaccinations is challenging. To overcome this, we rationally select the highly conserved peptides from the consensus amino acid sequence of the globular head domains of neuraminidase. Inspired by the B cell receptor evolution process, a reliable sequential immunization regimen is designed to result in immuno-focusing by steering bulk immune responses to a selected region where broadly protective B lymphocyte epitopes reside. After priming neuraminidase protein-specific antibody responses in C57BL/6 or BALB/c inbred mice strains by immunization or pre-infection, boost immunizations with certain neuraminidase-derived peptide-keyhole limpet hemocyanin conjugates significantly strengthened serum neuraminidase inhibition activities and cross-protections. Overall, this study provides proof of concept for a peptide-based sequential immunization strategy for achieving targeted induction of cross-protective antibody response, which provides references for designing universal vaccines against other highly variable pathogens.
Keywords: B cell receptor; CP: Immunology; cross-protection; influenza neuraminidase; peptide vaccine; sequential immunization; universal vaccine.
. 2023 Jul 7;42(7):112766.
doi: 10.1016/j.celrep.2023.112766. Online ahead of print. Boost immunizations with NA-derived peptide conjugates achieve induction of NA inhibition antibodies and heterologous influenza protections
De-Jian Liu[SUP] 1 [/SUP], Cui-Cui Liu[SUP] 1 [/SUP], Xiu-Qin Zhong[SUP] 1 [/SUP], Xuan Wu[SUP] 1 [/SUP], Hui-Hui Zhang[SUP] 2 [/SUP], Shang-Wen Lu[SUP] 2 [/SUP], Zhuo-Ling Shen[SUP] 1 [/SUP], Wen-Wen Song[SUP] 1 [/SUP], Shi-Long Zhao[SUP] 1 [/SUP], You-Song Peng[SUP] 3 [/SUP], He-Ping Zheng[SUP] 3 [/SUP], Mu-Yang Wan[SUP] 1 [/SUP], Yao-Qing Chen[SUP] 4 [/SUP], Lei Deng[SUP] 5 [/SUP]
Affiliations
- PMID: 37421618
- DOI: 10.1016/j.celrep.2023.112766
Neuraminidase is suggested as an important component for developing a universal influenza vaccine. Targeted induction of neuraminidase-specific broadly protective antibodies by vaccinations is challenging. To overcome this, we rationally select the highly conserved peptides from the consensus amino acid sequence of the globular head domains of neuraminidase. Inspired by the B cell receptor evolution process, a reliable sequential immunization regimen is designed to result in immuno-focusing by steering bulk immune responses to a selected region where broadly protective B lymphocyte epitopes reside. After priming neuraminidase protein-specific antibody responses in C57BL/6 or BALB/c inbred mice strains by immunization or pre-infection, boost immunizations with certain neuraminidase-derived peptide-keyhole limpet hemocyanin conjugates significantly strengthened serum neuraminidase inhibition activities and cross-protections. Overall, this study provides proof of concept for a peptide-based sequential immunization strategy for achieving targeted induction of cross-protective antibody response, which provides references for designing universal vaccines against other highly variable pathogens.
Keywords: B cell receptor; CP: Immunology; cross-protection; influenza neuraminidase; peptide vaccine; sequential immunization; universal vaccine.