tetano
Editor, Senior Moderator
Cell Rep
. 2022 Jan 31;110393.
doi: 10.1016/j.celrep.2022.110393. Online ahead of print.
B cell receptor repertoire kinetics after SARS-CoV-2 infection and vaccination
Prasanti Kotagiri[SUP] 1 [/SUP], Federica Mescia[SUP] 2 [/SUP], William M Rae[SUP] 2 [/SUP], Laura Bergamaschi[SUP] 2 [/SUP], Zewen K Tuong[SUP] 3 [/SUP], Lorinda Turner[SUP] 2 [/SUP], Kelvin Hunter[SUP] 2 [/SUP], Pehuén P Gerber[SUP] 2 [/SUP], Myra Hosmillo[SUP] 4 [/SUP], Cambridge Institute of Therapeutic Immunology and Infectious Disease-National Institute of Health Research (CITIID-NIHR) COVID BioResource Collaboration; Christoph Hess[SUP] 5 [/SUP], Menna R Clatworthy[SUP] 6 [/SUP], Ian G Goodfellow[SUP] 4 [/SUP], Nicholas J Matheson[SUP] 7 [/SUP], Eoin F McKinney[SUP] 2 [/SUP], Mark R Wills[SUP] 2 [/SUP], Ravindra K Gupta[SUP] 2 [/SUP], John R Bradley[SUP] 8 [/SUP], Rachael J M Bashford-Rogers[SUP] 9 [/SUP], Paul A Lyons[SUP] 10 [/SUP], Kenneth G C Smith[SUP] 11 [/SUP]
Affiliations
Abstract
B cells are important in immunity to both severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection and vaccination, but B cell receptor (BCR) repertoire development in these contexts has not been compared. We analyze serial samples from 171 SARS-CoV-2-infected individuals and 63 vaccine recipients and find the global BCR repertoire differs between them. Following infection, immunoglobulin (Ig)G1/3 and IgA1 BCRs increase, somatic hypermutation (SHM) decreases, and, in severe disease, IgM and IgA clones are expanded. In contrast, after vaccination, the proportion of IgD/M BCRs increase, SHM is unchanged, and expansion of IgG clones is prominent. VH1-24, which targets the N-terminal domain (NTD) and contributes to neutralization, is expanded post infection except in the most severe disease. Infection generates a broad distribution of SARS-CoV-2-specific clones predicted to target the spike protein, while a more focused response after vaccination mainly targets the spike's receptor-binding domain. Thus, the nature of SARS-CoV-2 exposure differentially affects BCR repertoire development, potentially informing vaccine strategies.
Keywords: B cell receptor repertoire; COVID-19; SARS-CoV-2 vaccination.
. 2022 Jan 31;110393.
doi: 10.1016/j.celrep.2022.110393. Online ahead of print.
B cell receptor repertoire kinetics after SARS-CoV-2 infection and vaccination
Prasanti Kotagiri[SUP] 1 [/SUP], Federica Mescia[SUP] 2 [/SUP], William M Rae[SUP] 2 [/SUP], Laura Bergamaschi[SUP] 2 [/SUP], Zewen K Tuong[SUP] 3 [/SUP], Lorinda Turner[SUP] 2 [/SUP], Kelvin Hunter[SUP] 2 [/SUP], Pehuén P Gerber[SUP] 2 [/SUP], Myra Hosmillo[SUP] 4 [/SUP], Cambridge Institute of Therapeutic Immunology and Infectious Disease-National Institute of Health Research (CITIID-NIHR) COVID BioResource Collaboration; Christoph Hess[SUP] 5 [/SUP], Menna R Clatworthy[SUP] 6 [/SUP], Ian G Goodfellow[SUP] 4 [/SUP], Nicholas J Matheson[SUP] 7 [/SUP], Eoin F McKinney[SUP] 2 [/SUP], Mark R Wills[SUP] 2 [/SUP], Ravindra K Gupta[SUP] 2 [/SUP], John R Bradley[SUP] 8 [/SUP], Rachael J M Bashford-Rogers[SUP] 9 [/SUP], Paul A Lyons[SUP] 10 [/SUP], Kenneth G C Smith[SUP] 11 [/SUP]
Affiliations
- PMID: 35143756
- PMCID: PMC8801326
- DOI: 10.1016/j.celrep.2022.110393
Abstract
B cells are important in immunity to both severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection and vaccination, but B cell receptor (BCR) repertoire development in these contexts has not been compared. We analyze serial samples from 171 SARS-CoV-2-infected individuals and 63 vaccine recipients and find the global BCR repertoire differs between them. Following infection, immunoglobulin (Ig)G1/3 and IgA1 BCRs increase, somatic hypermutation (SHM) decreases, and, in severe disease, IgM and IgA clones are expanded. In contrast, after vaccination, the proportion of IgD/M BCRs increase, SHM is unchanged, and expansion of IgG clones is prominent. VH1-24, which targets the N-terminal domain (NTD) and contributes to neutralization, is expanded post infection except in the most severe disease. Infection generates a broad distribution of SARS-CoV-2-specific clones predicted to target the spike protein, while a more focused response after vaccination mainly targets the spike's receptor-binding domain. Thus, the nature of SARS-CoV-2 exposure differentially affects BCR repertoire development, potentially informing vaccine strategies.
Keywords: B cell receptor repertoire; COVID-19; SARS-CoV-2 vaccination.