tetano
Editor, Senior Moderator
Infect Immun. 2013 Sep 30. [Epub ahead of print]
Capsular sialic acid of Streptococcus suis serotype 2 binds to swine influenza virus and enhances bacterial interactions with virus-infected tracheal epithelial cells.
Wang Y, Gagnon CA, Savard C, Music N, Srednik M, Segura M, Lachance C, Bellehumeur C, Gottschalk M.
Source
Facult? de m?decine v?t?rinaire, Universit? de Montr?al, 3200 Sicotte, St-Hyacinthe, Qu?bec, Canada J2S 2M2.
Abstract
Streptococcus suis serotype 2 is an important swine bacterial pathogen and it is also an emerging zoonotic agent. It is unknown how S. suis virulent strains, which are usually found in low quantities in pig tonsils, manage to cross the first host defense lines to initiate systemic disease. Influenza virus produces a contagious infection in pigs which is frequently complicated by bacterial co-infections leading to significant economic impacts. In this study, the effect of a preceding swine influenza H1N1 virus (swH1N1) infection of swine tracheal epithelial cells (NTPr) on the ability of S. suis serotype 2 to adhere, invade and activate these cells was evaluated. Cells pre-infected with swH1N1, showed bacterial adhesion and invasion levels increased more than 100 fold when compared to normal cells. Inhibition studies confirmed that the capsular sialic acid moiety is responsible for the binding to virus-infected cell surface. Also, pre-incubation of S. suis with swH1N1 significantly increased bacterial adhesion/invasion to epithelial cells, suggesting that S. suis may also use swH1N1 as a vehicle to invade epithelial cells when the two infections occur simultaneously. Influenza infection may facilitate the transient passage of S. suis at the respiratory tract to reach the bloodstream and cause bacteremia and septicemia. S. suis may also increase the local inflammation at the respiratory tract during influenza infection, as suggested by an exacerbated expression of pro-inflammatory mediators in co-infected cells. These results give a new insight in the elucidation of complex interactions between influenza virus and S. suis in a co-infection model.
PMID:
24082069
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24082069
Capsular sialic acid of Streptococcus suis serotype 2 binds to swine influenza virus and enhances bacterial interactions with virus-infected tracheal epithelial cells.
Wang Y, Gagnon CA, Savard C, Music N, Srednik M, Segura M, Lachance C, Bellehumeur C, Gottschalk M.
Source
Facult? de m?decine v?t?rinaire, Universit? de Montr?al, 3200 Sicotte, St-Hyacinthe, Qu?bec, Canada J2S 2M2.
Abstract
Streptococcus suis serotype 2 is an important swine bacterial pathogen and it is also an emerging zoonotic agent. It is unknown how S. suis virulent strains, which are usually found in low quantities in pig tonsils, manage to cross the first host defense lines to initiate systemic disease. Influenza virus produces a contagious infection in pigs which is frequently complicated by bacterial co-infections leading to significant economic impacts. In this study, the effect of a preceding swine influenza H1N1 virus (swH1N1) infection of swine tracheal epithelial cells (NTPr) on the ability of S. suis serotype 2 to adhere, invade and activate these cells was evaluated. Cells pre-infected with swH1N1, showed bacterial adhesion and invasion levels increased more than 100 fold when compared to normal cells. Inhibition studies confirmed that the capsular sialic acid moiety is responsible for the binding to virus-infected cell surface. Also, pre-incubation of S. suis with swH1N1 significantly increased bacterial adhesion/invasion to epithelial cells, suggesting that S. suis may also use swH1N1 as a vehicle to invade epithelial cells when the two infections occur simultaneously. Influenza infection may facilitate the transient passage of S. suis at the respiratory tract to reach the bloodstream and cause bacteremia and septicemia. S. suis may also increase the local inflammation at the respiratory tract during influenza infection, as suggested by an exacerbated expression of pro-inflammatory mediators in co-infected cells. These results give a new insight in the elucidation of complex interactions between influenza virus and S. suis in a co-infection model.
PMID:
24082069
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24082069