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Canada - LPAI H5 Confirmed on 2nd Turkey Farm

Re: Canada - LPAI H5 Confirmed on Turkey Farm

Re: Canada - LPAI H5 Confirmed on Turkey Farm

did they determine the N-type ?
did they determine what viruses are similar ?
(Mexican H5N2, USA H5N1, German H5N3 ?)

((((when)will they give us sequences??))))
 
Re: Canada - LPAI H5 Confirmed on Turkey Farm

Re: Canada - LPAI H5 Confirmed on Turkey Farm

<TABLE border=0 cellSpacing=0 cellPadding=0><TBODY><TR><TD class=roundHeaderText images target="_blank" href="http://www.ctv.ca" roundAMBlueheadermiddle.jpg);? local990 mar TD <><TD images target="_blank" href="http://www.ctv.ca" local990 mar TD <></TD><TR><TD images target="_blank" href="http://www.ctv.ca" local990 mar </TD><TD class=middleBodyRoundAM>The Canadian Food Inspection Agency confirmed an H5 avian influenza outbreak on a turkey farm in B.C.'s Fraser Valley on Saturday, Jan. 24, 2009.

<TABLE id=morePhotos><TBODY><TR><TD>A barn containing some of the 60,000 turkeys on E&H Farms in Abbotsford, BC is idle after turkeys on the farm tested positive for the H5 avian flu virus, Sunday, Jan. 25, 2009. (Richard Lam / THE CANADIAN PRESS)

A bio hazard control sign sits outside a poultry farm in Abbotsford, B.C. after a turkey farm in the area tested positive for the H5 avian flu virus, Saturday, January 24, 2009. (Richard Lam / THE CANADIAN PRESS)




</TD></TR></TBODY></TABLE>Nine new farms quarantined for avian flu

Updated: Sun Feb. 01 2009 16:56:56

<SCRIPT language=javascript type=text/javascript> var byString = ""; var sourceString = "The Canadian Press"; if ((sourceString != "") && (byString != "")) { document.write(byString + ", "); } else { document.write(byString); }</SCRIPT>The Canadian Press

VANCOUVER -- Three of four of the most recent avian flu outbreaks in Canada have broken out in British Columbia's Fraser Valley but despite years of trying to figure it out, they still can't explain why the valley attracts the virus.

In the latest outbreak, 60,000 turkeys were culled on an Abbotsford, B.C., farm last week.
Tests so far indicate the virus has not spread to any other poultry producers within a three-kilometre quarantine zone.

However, the Canadian Food Inspection Agency said Sunday it has quarantined seven premises outside the zone and two more inside, based on an analysis of the movement of people, products and equipment.

In all, there are 36 premises under quarantine but the agency says so far all of those flocks have tested negative for the virus.

This is the third avian-flu outbreak in the Fraser Valley in the last few years -- a frequency scientists believe is related to the high-concentration of poultry operations in the valley and its location under the Pacific migratory flyway for wild birds that carry the disease.

That wasn't the case in the valley's first outbreak in 2004 when an H7-type flu transformed into a highly contagious strain.

Farm after farm was quarantined until finally about 15 million birds -- almost the entire valley poultry population -- was destroyed.

The second Fraser Valley outbreak in November 2005 saw two duck farms infected with the H5N2 strain of the virus.

In 2007, a highly pathogenic H7N3 strain was found in Saskatchewan on a farm that produced hatching eggs to produce broiler chickens.
Experts have difficulty explaining why the Fraser Valley has been hit so often but there are theories.

"We're on the Pacific flyway so there are lots of birds passing through every year," says Ron Lewis, the chief veterinary officer for British Columbia.
"And we know wild waterfowl carry a variety of different strains of avian influenza."

People can easily walk over the areas where wild waterfowl have been.

"And we know avian influenza has some capability of aerosol transmission," he says.

Lewis and Sandra Stephens, veterinary program specialist with the Canadian Food Inspection Agency, suspect the main culprits are the wild birds moving throughout North America and the world on migratory flyways.

Avian influenza virus is prolific and when wild waterfowl have it "they shed tremendous numbers of viral particles."

"All these birds when they get to their summer or wintering grounds they co-mingle," says Stephens.
And North American flyways are not completely isolated from others.

"Although you tend to think of the North American flyways as being separate from Europe and Asia, in fact there is some crossover in the northern breeding grounds," she says.

The huge concentration of commercial poultry producers in the Fraser Valley is also a factor in the spread.

Any poultry producer faces being exposed to migratory birds.

"But where we have more concerns are areas like the Fraser Valley or the Niagara Peninsula where there are large concentrations of poultry farms close together," says Stephens.

Calvin Breukelman of the B.C. Poultry Association estimates there are about 600 poultry producers in the Fraser Valley in an industry worth close to $1 billion.

Such concentration occurred for obvious economic reasons: close access to a large market.

"In an ideal world we of course wouldn't have this concentration but it's not ideal and we have to do whatever we can to reduce the impact of that concentration," says Lewis.

Perhaps the biggest worry facing producers and the general population is the likelihood of the highly pathogenic H5N1 virus that killed hundreds of people in Asia, Africa and Europe occurring here.

Lewis suggests it's highly unlikely.
In countries where H5N1 occurred there are very close living conditions between poultry and people, he says.

"We don't have that kind of proximity to wild and commercial ducks co-habiting in many cases that is present in those countries," he says.

Moreover, Lewis says, the intense biosecurity measures introduced in Canada after the 2004 Fraser Valley outbreak are not practised in many of those countries.

"We now have a mandatory biosecurity program in place for all of the commercially-regulated poultry industry," he says.

"The industry has achieved a very high level of compliance with those biosecurity protocols," says Lewis, estimating the compliance rate at about 98 per cent of the industry.

Initial testing for avian viruses also can now be done in Abbotsford.

"So we have eliminated the delays in having to ship samples for testing at the federal lab," says Lewis.

Poultry producers in the Fraser Valley are still awaiting tests on the latest outbreak determining how contagious the virus may be.

"It sometimes can take a number of weeks to get the result," says Stephens.

The presence of H5 virus has been established but its specificity, or neuraminidase subtype which is the N in the virus name, isn't known yet.

That H5 virus was detected in some turkeys on a property owned by two brothers last month.
The avian flu has many different sub types with 16 different H's, or hemagglutinin, and nine different N's, meaning there are 144 possible combinations of the flu virus.

The virus must be grown in a lab culture and the latest sample has taken longer than expected.
"Some viruses grow (in a lab culture) more readily than others and there's no way of knowing which virus will grow quicker than another," says Stephens.






</TD></TR></TBODY></TABLE>

http://www.ctvbc.ctv.ca/servlet/an/...avian_flu_090201/20090201?hub=BritishColumbia
 
Last edited by a moderator:
Re: Canada - LPAI H5 Confirmed on Turkey Farm

Re: Canada - LPAI H5 Confirmed on Turkey Farm

did they determine the N-type ?
did they determine what viruses are similar ?
(Mexican H5N2, USA H5N1, German H5N3 ?)

((((when)will they give us sequences??))))

From the original CN article:

"Initial testing for avian viruses also can now be done in Abbotsford.

"So we have eliminated the delays in having to ship samples for testing at the federal lab," says Lewis.

Poultry producers in the Fraser Valley are still awaiting tests on the latest outbreak determining how contagious the virus may be.

"It sometimes can take a number of weeks to get the result," says Stephens.

The presence of H5 virus has been established but its specificity, or neuraminidase subtype which is the N in the virus name, isn't known yet."

So we know that they don't know the N type yet.

Can we assume that "initial testing" means that sequences and virus similarity cannot be determined in Abbotsford?

If so, then the samples would go to Winnipeg, I guess. So add 2 days for packing, transport, and unpacking. Then add the expected time for conducting the appropriate tests. What are these expected times? Then add some time for the administration to get up to speed. So adding together those durations, when can the disclosure of the results reasonbly be expected?

J.
 
Re: Canada - LPAI H5 Confirmed on Turkey Farm

Re: Canada - LPAI H5 Confirmed on Turkey Farm

Low pathogenic avian influenza (poultry), Canada (2/2/2009) [WAHID Interface - OIE]

Low pathogenic avian influenza (poultry), Canada

Information received on 31/01/2009 from Dr Brian Evans, Chief Veterinary Officer, 59 Camelot Drive, Room 146 W, Canadian Food Inspection Agency, OTTAWA, Canada

-- Summary
Report type Follow-up report No. 1
Start date 12/01/2009
Date of first confirmation of the event 23/01/2009
Report date 31/01/2009
Date submitted to OIE 31/01/2009
Reason for notification Reoccurrence of a listed disease
Date of previous occurrence 2005
Manifestation of disease Clinical disease
Causal agent Low pathogenic avian influenza virus (H5) Serotype Pending
Nature of diagnosis Laboratory (advanced)
This event pertains to a defined zone within the country
Related reports
* Immediate notification (24/01/2009)
* Follow-up report No. 1 (31/01/2009)

-- Outbreaks
There are no new outbreaks in this report

-- Epidemiology
Source of the outbreak(s) or origin of infection
* Unknown or inconclusive

-- Epidemiological comments
- All birds on the infected premises have been humanely depopulated on 26-27 January 2009 and carcasses will be destroyed on site by composting.
- The premises will remain under quarantine until 21 days following the completion of CFIA approved cleaning and disinfection.
- All activities are under the direct supervision of the Canadian Food Inspection Agency (CFIA).
- Surveillance and control activities continue as defined in the CFIA Notifiable Avian Influenza Hazard Specific Plan.
- To date, there are a total of thirty-five quarantines in place, ten of which are outside of the 3km zone from the infected premises.
- Additional quarantines for contact premises may be issued as the epidemiological investigation and tracing of the movement of people, equipment and products continues.
- Tests so far have not detected the presence of virus on other premises within the 3km zone or on a high risk premises.
- Several attempts to isolate the virus from the index farm have not been successful yet which is an indication of the very low level of infection.
- Additional testing at the CFIA National Centre for Foreign Animal Diseases is underway in order to characterize the virus.
Source of infection is still inconclusive.

Note by the OIE Animal Health Information Department: H5 and H7 avian influenza in its low pathogenic form in poultry is a notifiable disease as per Chapter 10.4. on avian influenza of the Terrestrial Animal Health Code (2008).

-- Control measures
Measures applied
* Stamping out
* Quarantine
* Screening
* Vaccination prohibited
* No treatment of affected animals
Measures to be applied
* Disinfection of infected premises/establishment(s)

-- Diagnostic test results
Laboratory name and type CFIA National Center for Foreign Animal Diseases (National laboratory)
Tests and results: Species - Test - Test date - Result
- Birds - competitive ELISA (c-ELISA) - 24/01/2009 - Positive
- Birds - virus isolation - 30/01/2009 - Pending

-- Future Reporting
The event is continuing. Weekly follow-up reports will be submitted.
-
<cite cite="http://www.oie.int/wahis/public.php?page=single_report&pop=1&reportid=7745">WAHID Interface - OIE World Animal Health Information Database</cite>
 
Re: Canada - LPAI H5 Confirmed on Turkey Farm

Re: Canada - LPAI H5 Confirmed on Turkey Farm

"- Several attempts to isolate the virus from the index farm have not been successful yet which is an indication of the very low level of infection.
- Additional testing at the CFIA National Centre for Foreign Animal Diseases is underway in order to characterize the virus. "

Is the first statement true?

The lab mentioned is Winnipeg:

http://www.inspection.gc.ca/english/sci/lab/csce.shtml

J.
 
Re: Canada - LPAI H5 Confirmed on Turkey Farm

Re: Canada - LPAI H5 Confirmed on Turkey Farm

Source: http://www.vancouversun.com/Health/Avian+contained+farm+CFIA/1244465/story.html

Avian flu contained to one B.C. farm: CFIA
Canwest News Service

OTTAWA -- Officials testing flocks of turkeys within a three-kilometre radius of an Abbotsford, B.C., turkey farm found contaminated with avian influenza this week say there is no evidence that the virus has spread.

The Canadian Food Inspection Agency says all commercial flocks of turkeys at 23 neighbouring farms in the Vancouver suburb have tested negative for the H5 strain of avian flu.

Testing and monitoring of the farms will continue for a 21-day period.

The nearby farms remain under quarantine, the agency said.

Earlier this week, approximately 60,000 birds on the infected farm were killed with carbon dioxide gas. Cleanup crews have begun composting the carcasses at the two barns to ensure the virus is rendered inactive.

After seven to 10 days, remains will then be removed from the barn to complete the composting process.

In 2004, 17 million chickens were culled in B.C.'s Fraser Valley, the largest cull in Canadian history. No humans were injured or died as a result from this outbreak.

Avian flu rarely affects humans unless the person has had close contact with the infected birds. More than 250 people worldwide have died after contracting the H5N1 subtype of avian flu.
 
Re: Canada - LPAI H5 Confirmed on Turkey Farm

Re: Canada - LPAI H5 Confirmed on Turkey Farm

"- Several attempts to isolate the virus from the index farm have not been successful yet which is an indication of the very low level of infection.
- Additional testing at the CFIA National Centre for Foreign Animal Diseases is underway in order to characterize the virus. "

Is the first statement true?

The lab mentioned is Winnipeg:

http://www.inspection.gc.ca/english/sci/lab/csce.shtml

J.
It's beginning to sound like the dog ate the homework AGAIN!
 
Re: Canada - LPAI H5 Confirmed on Turkey Farm

Re: Canada - LPAI H5 Confirmed on Turkey Farm

Follow-up report No.: 1​
Report reference: 2009-CAN-AI-01, OIE Ref: 7745, Report Date: 31/01/2009, Country: Canada​
Report Summary​
Disease​
Low pathogenic avian influenza (poultry) Animal type Terrestrial

Causal Agent​
Low pathogenic avian influenza virus (H5) Serotype(s) Pending

Clinical Signs​
Yes Reason Reoccurrence of a listed disease

Date of first confirmation of the event​
23/01/2009 Date of Start of Event 12/01/2009

Date of report​
31/01/2009 Date Submitted To OIE 31/01/2009

Diagnosis​
Laboratory (advanced) Date Of Last Occurrence 2005

Number Of Reported Outbreaks​
Submitted= 1, Draft= 0, Deleted= 0 Name of Sender of the report Dr Brian Evans

Address​
59 Camelot Drive, Room 146 W OTTAWA K1A 0Y9 Position Chief Veterinary Officer

Telephone​
1 613 225 2342 Fax 1 613 228 6126

Email​
bevans@inspection.gc.ca Entered by Dr Brian Evans

Outbreak (other report - draft) (deleted) ( 2009-BC-001a )​
Province Unit Type Location Latitude Longitude Start date End Date​
BRITISH COLUMBIA Farm Abbotsford 49,0345 -122,4538 12/01/2009​
Species Measuring units Susceptible Cases Deaths Destroyed Slaughtered​
Birds Animals 29565 1 0 25000 0​
Affected Population​
Turkey meat type production. This premises is adjacent to the index farm (BC-001) and these two farms are owned by the same family. The barns are very close one to the other (less than 100 meters apart) and share common driveways. Because of the nature of their activities and the strong
relationship between them, they are considered to be the same epidemiological unit.​
Outbreak (other report - submitted) ( 2009-BC-001 )​
Province Unit Type Location Latitude Longitude Start date End Date​
BRITISH COLUMBIA Farm Abbotsford 49,0338 -122,4541 12/01/2009​
Species Measuring units Susceptible Cases Deaths Destroyed Slaughtered​
Birds Animals 57339 1300 0 57339 0​
Affected Population​
A turkey meat type production unit. Turkeys are all kept in the same barn. There are 3 distinct groups of birds. The affected flock is 90 days old (8,755 birds) with a morbidity about 15% and the other groups are 20 days (9,245 birds) and 55 days (9,774 birds). No significant mortality was
observed.
On the same premises, in another barn, there are three other groups of meat type turkeys: one is 7 days old (10,151 birds), another is 50 days old (9,631 birds) and the last one is 84 days old (9,783 birds). No clinical signs were observed in that barn.​
Outbreak summary: Total outbreaks = 1 (Draft)​
Printed on: Mon Feb 2 16:11:34 Paris, Madrid 2009 Page 1/3​
Species Susceptible Cases Deaths Destroyed Slaughtered​
Birds 29565 1 0 25000 0​
Outbreak summary: Total outbreaks = 1 (Submitted)​
Species Susceptible Cases Deaths Destroyed Slaughtered​
Birds 57339 1300 0 57339 0​
Epidemiology​
Epidemiological comments​
All birds on the infected premises have been humanely depopulated on 26-27 January 2009 and carcasses will be destroyed on site by composting. The premises will remain under quarantine until 21 days following the completion of CFIA approved cleaning and disinfection. All activities are
under the direct supervision of the Canadian Food Inspection Agency (CFIA).
Surveillance and control activities continue as defined in the CFIA Notifiable Avian Influenza Hazard Specific Plan. To date, there are a total of thirty-five quarantines in place, ten of which are outside of the 3km zone from the infected premises. Additional quarantines for contact premises may
be issued as the epidemiological investigation and tracing of the movement of people, equipment and products continues. Tests so far have not detected the presence of virus on other premises within the 3km zone or on a high risk premises.
Several attempts to isolate the virus from the index farm have not been successful yet which is an indication of the very low level of infection. Additional testing at the CFIA National Centre for Foreign Animal Diseases is underway in order to characterize the virus. Source of infection is still
inconclusive.
Note by the OIE Animal Health Information Department: H5 and H7 avian influenza in its low pathogenic form in poultry is a notifiable disease as per Chapter 10.4. on avian influenza of the Terrestrial Animal Health Code (2008).​
Source of the outbreak(s) or origin of infection​
? Unknown or inconclusive

Control Measures​
Applied​
? Stamping out
? Quarantine
? Screening

To be applied​
? Disinfection of infected premises/establishment(s)

Animals treated​
No

Vaccination Prohibited​
Yes

Country / Zone​
Country or zone​
a zone or compartment

Diagnostic test results​
Laboratory Type Name of Laboratory Species Test Type Date Results Provided Result​
National laboratory CFIA National Center for Foreign Animal Diseases Birds virus isolation 30/01/2009 Pending
National laboratory CFIA National Center for Foreign Animal Diseases Birds competitive ELISA (c-ELISA) 24/01/2009 Positive​
Future Reporting​
What further reports will be submitted in relation to this event?​
There are 1outbreaks that are still recorded as unresolved. It is not possible to declare this event resolved until these individual outbreaks are resolved.​
The event is continuing. Weekly follow-up reports will be submitted.

http://www.oie.int/wahis/reports/en_fup_0000007745_20090131_161134.pdf
 
Re: Canada - LPAI H5 Confirmed on Turkey Farm

Re: Canada - LPAI H5 Confirmed on Turkey Farm

:smartass:Low pathogenic avian influenza virus (H5) Serotype(s) Pending:rolleyes:
 
Re: Canada - LPAI H5 Confirmed on Turkey Farm

Re: Canada - LPAI H5 Confirmed on Turkey Farm

:smartass:Low pathogenic avian influenza virus (H5) Serotype(s) Pending:rolleyes:

<ACRONYM title="Canadian Food Inspection Agency">CFIA</ACRONYM> News

February 3, 2009

  • <LI class=doubleLineBullet>Tests conducted by the National Centre for Foreign Animal Diseases in Winnipeg indicate that the H5 avian influenza virus detected in British Columbia is H5N2. Preliminary tests also indicate that the strain of the virus is of low pathogenicity. <LI class=doubleLineBullet>Avian influenza is a disease of birds that does not cross easily from birds to infect humans. There is no evidence to date that shows this strain poses any significant risk to human health. <LI class=doubleLineBullet>In-barn composting continues. Temperatures are being monitored inside the compost piles to ensure the virus is effectively destroyed. These procedures are consistent with provincial environmental regulations and internationally accepted disease control guidelines. <LI class=doubleLineBullet>Thirty-six premises remain under quarantine through the investigation. These premises are either located within the 3-kilometer radius of the infected premises or have had some known contact with the infected premises during the time the virus may have been present. These premises have been quarantined as a precautionary measure. <LI class=doubleLineBullet>Prior to moving poultry or poultry products off the quarantined premises, birds must be sampled, tested negative and a license for the movement must be issued by the CFIA.
  • Poultry owners in the area are encouraged to take an active role in protecting their flocks by immediately reporting any signs of illness that could be consistent with avian influenza by calling (604) 226-1753.
http://www.inspection.gc.ca/english/anima/heasan/disemala/avflu/situatione.shtml
 
Re: Canada - LPAI H5 Confirmed on Turkey Farm

Re: Canada - LPAI H5 Confirmed on Turkey Farm

Canada. B.C. bird flu is H5N2, likely "low-path"

B.C. bird flu is H5N2, likely "low-path"

Staff2/4/2009 1:42:00 AM

The avian influenza that led federal inspectors to cull, gas and compost about 60,000 birds on a Fraser Valley poultry farm in British Columbia is subtype H5N2, and likely low in pathogenicity.


The Canadian Food Inspection Agency reported Tuesday that tests at the National Centre for Foreign Animal Diseases in Winnipeg confirm the H5 bird flu virus detected on the farm is H5N2.

The agency also reiterated that preliminary tests at the outset of its investigation on Jan. 24 indicate this strain of bird flu is of low pathogenicity ("low-path").

It's thus likely that Canada will keep its status as free of "high-path" bird flu, which it's held since April last year after the cleanup of an outbreak of H7N3 on a poultry farm near Regina Beach, Sask.

While bird flu, particularly high-path strains, can be devastating on an affected commercial poultry farm, the concern of human health experts is that a strain such as the notorious H5N1 may mutate or combine with a human flu virus that could spread more easily among people, spurring a pandemic.

H5N1 since 2003 has killed about 250 people overseas, generally through direct contact with infected birds or their fluids.

CFIA also reiterated that avian flu is "a disease of birds that does not cross easily from birds to infect humans." Furthermore, the agency said, "there is no evidence to date that shows this strain poses any significant risk to human health."

Quarantines continue as a "precautionary measure" on 36 properties near the Fraser Valley farm, CFIA said Tuesday. Of those, 26 are within a three-kilometre radius of the index farm. The remaining 10 are outside that radius but were identified for quarantine "as a result of the assessment of the movement of people, products and equipment through (CFIA's) investigation.

"Poultry or poultry products being moved off quarantined premises must first be submitted for samples, test negative and be cleared by CFIA. The agency is also encouraging poultry owners in the area are encouraged to "take an active role in protecting their flocks" and immediately report any signs of illness that could be consistent with avian influenza by calling 604-226-1753.
-
<cite cite="http://www.canadiancattlemen.ca/issues/ISArticle.asp?id=95567&issue=02042009&story_id=&PC=FBC">canadiancattlemen.ca - Cattlemen magazine is Canada??s publication and web site for cattle farmers. - Cattlemen magazine is Canada??s publication and web site for cattle farmers. - 2/4/2009</cite>
 
Re: Canada - LPAI H5 Confirmed on Turkey Farm

Re: Canada - LPAI H5 Confirmed on Turkey Farm

Low pathogenic avian influenza (poultry), Canada (1/26/2009) [WAHID Interface - OIE]
Low pathogenic avian influenza (poultry), Canada

Results of gene sequencing indicate a low path cleavage site (NVPQRETR/GLFGAIA).
The sequence is 99% related to another H5 virus (H5N2) from California in 2007.
Closest match in Genbank is A/American green-winged teal/California/HKWF609/2007(H5N2), accession number: CY033444
-
<CITE cite=http://www.oie.int/wahis/public.php?page=single_report&pop=1&reportid=7720>WAHID Interface - OIE World Animal Health Information Database</CITE>
It is worth noting that there have been delays in isolating the virus and getting a full serotype (which has now been called H5N2), but even in the absence of cultured virus, it was possible to get an HA cleavage site sequence as well as enough sequence to cite 99% identity to a prior isolate.
This is in marked contrast to the Prince Edward Island incident, where ducks died and were H5 PCR confirmed, but Winnepeg failed to confirm, no report was filed, and the size of the insert was withheld.
 
Re: Canada - LPAI H5 Confirmed on Turkey Farm

Re: Canada - LPAI H5 Confirmed on Turkey Farm

Low pathogenic avian influenza (poultry), Canada (2/4/2009) [WAHID Interface - OIE]

Low pathogenic avian influenza (poultry), Canada

Information received on 04/02/2009 from Dr Brian Evans, Chief Veterinary Officer, 59 Camelot Drive, Room 146 W, Canadian Food Inspection Agency, OTTAWA, Canada

-- Summary
Report type Follow-up report No. 2
Start date 12/01/2009
Date of first confirmation of the event 23/01/2009
Report date 03/02/2009
Date submitted to OIE 04/02/2009
Reason for notification Reoccurrence of a listed disease
Date of previous occurrence 2005
Manifestation of disease Clinical disease
Causal agent Low pathogenic avian influenza virus Serotype H5N2
Nature of diagnosis Laboratory (advanced)
This event pertains to a defined zone within the country
Related reports
* Immediate notification (24/01/2009)
* Follow-up report No. 1 (31/01/2009)
* Follow-up report No. 2 (03/02/2009)

-- Outbreaks
There are no new outbreaks in this report

-- Epidemiology
Source of the outbreak(s) or origin of infection
* Unknown or inconclusive
* Wild birds

-- Epidemiological comments
The CFIA National Centre for Foreign Animal Diseases (NCFAD) has now isolated a Notifiable Avian Influenza Virus (AIV) from the index farm.
The AIV isolate was confirmed as H5N2.
The closest match in Genbank for the N gene is A/American green-winged teal/California/HKWF406/2007(H5N2), accession number: CY033446.
The closest match in Genbank for the H gene is A/American green-winged teal/California/HKWF609/2007(H5N2), accession number: CY033444, as reported in the immediate notification.
Based on gene sequencing results, the virus was determined to be of low virulence.
The NCFAD will now proceed with the determination of the IVPI index.

Note by the OIE Animal Health Information Department: H5 and H7 avian influenza in its low pathogenic form in poultry is a notifiable disease as per Chapter 10.4. on avian influenza of the Terrestrial Animal Health Code (2008).

-- Control measures
Measures applied
* Stamping out
* Quarantine
* Screening
* Vaccination prohibited
* No treatment of affected animals
Measures to be applied
* Disinfection of infected premises/establishment(s)

-- Diagnostic test results
Laboratory name and type CFIA National Center for Foreign Animal Diseases (National laboratory)
Tests and results: Species - Test - Test date - Result
- Birds - gene sequencing - 02/02/2009 - Positive
- Birds - intravenous pathogenicity index (IVPI) test - 02/02/2009 - Pending
- Birds - virus isolation - 02/02/2009 - Positive

-- Future Reporting
The event is continuing. Weekly follow-up reports will be submitted.
-
<cite cite="http://www.oie.int/wahis/public.php?page=single_report&pop=1&reportid=7761">WAHID Interface - OIE World Animal Health Information Database</cite>
 
Re: Canada - LPAI H5 Confirmed on Turkey Farm

Re: Canada - LPAI H5 Confirmed on Turkey Farm

What is this?

"Birds - intravenous pathogenicity index (IVPI) test - 02/02/2009 - Pending"

J.
 
Re: Canada - LPAI H5 Confirmed on Turkey Farm

Re: Canada - LPAI H5 Confirmed on Turkey Farm

So can the teal be tracked backwards in time and space to describe, hopefully, the origins of the strain?

"The closest match in Genbank for the N gene is A/American green-winged teal/California/HKWF406/2007(H5N2), accession number: CY033446.

The closest match in Genbank for the H gene is A/American green-winged teal/California/HKWF609/2007(H5N2), accession number: CY033444, as reported in the immediate notification. "

J.
 
Re: Canada - LPAI H5 Confirmed on Turkey Farm

Re: Canada - LPAI H5 Confirmed on Turkey Farm

What is this?

"Birds - intravenous pathogenicity index (IVPI) test - 02/02/2009 - Pending"

J.

IPIV for the French-speaking people or IVPI ( Intravenous pathogenicity index) for the Anglo-Saxons is the acronym indicating the Index of intravenous pathogenicity.

To identify a Virus, one generally uses the RT-PCR practiced starting from sample of suspect bodies. It is a fast test (a few hours) of diagnosis, but which must be confirmed by viral insulation, more reliable.

In the case of the Avian flu for example, the virus must then be typified (H5 or H7.) and its given virulence. This virulence is measured by inoculation with chickens SPF (IPIV), and/or by sequencing of the site of cleavage of the protein H (basic residues).

http://www.speedylook.com/Index_of_intravenous_pathogenicity.html
 
Re: Canada - LPAI H5 Confirmed on Turkey Farm

Re: Canada - LPAI H5 Confirmed on Turkey Farm

. This virulence is measured by inoculation with chickens SPF (IPIV), and/or by sequencing of the site of cleavage of the protein H (basic residues).

http://www.speedylook.com/Index_of_intravenous_pathogenicity.html

O.k., thanks.

But how can the report state:

"Causal agent Low pathogenic avian influenza virus Serotype H5N2"

when the IPIV test that determines pathogenicity has yet to be completed?

J.
 
Re: Canada - LPAI H5 Confirmed on Turkey Farm

Re: Canada - LPAI H5 Confirmed on Turkey Farm

It could be that thses folks have a measurement of the HA1 segment, and if it is normal size (ie. no additional amino acids), then it is likely low path. Also, judging by the affect of this virus on the flock (respiratory distress, but no deaths), it is also likely low path.

However, the H5N2 detected at the Texas chicken farm some years ago had a lenghtened HA with additional basic amino acids at the cleavage site, and it had killed a number of the hens. After testing the isolated virus against the 10 day old chicks (standard test for pathogenicity), it was deemed low path. In other words, not enough chicks died to warrant the High Path tag.

Point is, any H5 or H7 infection of poultry needs to be carefully handled as history has shown that this stuff goes High Path really quickly if left to its own devices.
 
Re: Canada - LPAI H5 Confirmed on Turkey Farm

Re: Canada - LPAI H5 Confirmed on Turkey Farm

P.S.: I've been told that the Canadian virus has been sequenced, at least as to the HA segment, so they know precisely what the cleavage site looks like. This means that there are no additional amino acids, a good indicator of low pathogenicity.
 
Re: Canada - LPAI H5 Confirmed on Turkey Farm

Re: Canada - LPAI H5 Confirmed on Turkey Farm

P.S.: I've been told that the Canadian virus has been sequenced, at least as to the HA segment, so they know precisely what the cleavage site looks like. This means that there are no additional amino acids, a good indicator of low pathogenicity.
The cleavage site was published ago.
 
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