tetano
Editor, Senior Moderator
Braz J Microbiol
. 2022 Jul 1.
doi: 10.1007/s42770-022-00789-z. Online ahead of print.
Viral isolation allows characterization of early samples of SARS-CoV-2 lineage B1.1.33 with unique mutations (S: H655Y and T63N) circulating in Southern Brazil in 2020
Juliana Schons Gularte[SUP] 1 [/SUP], Mariana Soares da Silva[SUP] 2 [/SUP], Micheli Filippi[SUP] 2 [/SUP], Meriane Demoliner[SUP] 2 [/SUP], Karoline Schallenberger[SUP] 2 [/SUP], Alana Witt Hansen[SUP] 2 [/SUP], Vyctoria Malayhka de Abreu Góes Pereira[SUP] 2 [/SUP], Fágner Henrique Heldt[SUP] 2 [/SUP], Viviane Girardi[SUP] 2 [/SUP], Matheus Nunes Weber[SUP] 2 [/SUP], Paula Rodrigues de Almeida[SUP] 2 [/SUP], Bruno Lopes Abbadi[SUP] 3 [/SUP], Maiele Dornelles[SUP] 3 [/SUP], Cristiano Valim Bizarro[SUP] 3 [/SUP], Pablo Machado[SUP] 3 [/SUP], Luiz Augusto Basso[SUP] 3 [/SUP], Odir Antonio Dellagostin[SUP] 4 [/SUP], Juliane Deise Fleck[SUP] 2 [/SUP], Fernando Rosado Spilki[SUP] 2 [/SUP]
Affiliations
Abstract
Different approaches are in use to improve our knowledge about the causative agent of coronavirus disease (COVID-19). Cell culture-based methods are the better way to perform viral isolation, evaluate viral infectivity, and amplify the virus. Furthermore, next-generation sequencing (NGS) have been essential to analyze a complete genome and to describe new viral species and lineages that have arisen over time. Four naso-oropharyngeal swab samples, collected from April to July of 2020, were isolated and sequenced aiming to produce viral stocks and analyze the mutational profile of the found lineage. B.1.1.33 was the lineage detected in all sequences. Although the samples belong to the same lineage, it was possible to evaluate different mutations found including some that were first described in these sequences, like the S:H655Y and T63N. The results described here can help to elicit how the pandemic started to spread and how it has been evolving in south Brazil.
Keywords: B.1.1.33; S:H655Y; S:T63N; SARS-CoV-2; Southern Brazil.
. 2022 Jul 1.
doi: 10.1007/s42770-022-00789-z. Online ahead of print.
Viral isolation allows characterization of early samples of SARS-CoV-2 lineage B1.1.33 with unique mutations (S: H655Y and T63N) circulating in Southern Brazil in 2020
Juliana Schons Gularte[SUP] 1 [/SUP], Mariana Soares da Silva[SUP] 2 [/SUP], Micheli Filippi[SUP] 2 [/SUP], Meriane Demoliner[SUP] 2 [/SUP], Karoline Schallenberger[SUP] 2 [/SUP], Alana Witt Hansen[SUP] 2 [/SUP], Vyctoria Malayhka de Abreu Góes Pereira[SUP] 2 [/SUP], Fágner Henrique Heldt[SUP] 2 [/SUP], Viviane Girardi[SUP] 2 [/SUP], Matheus Nunes Weber[SUP] 2 [/SUP], Paula Rodrigues de Almeida[SUP] 2 [/SUP], Bruno Lopes Abbadi[SUP] 3 [/SUP], Maiele Dornelles[SUP] 3 [/SUP], Cristiano Valim Bizarro[SUP] 3 [/SUP], Pablo Machado[SUP] 3 [/SUP], Luiz Augusto Basso[SUP] 3 [/SUP], Odir Antonio Dellagostin[SUP] 4 [/SUP], Juliane Deise Fleck[SUP] 2 [/SUP], Fernando Rosado Spilki[SUP] 2 [/SUP]
Affiliations
- PMID: 35778549
- DOI: 10.1007/s42770-022-00789-z
Abstract
Different approaches are in use to improve our knowledge about the causative agent of coronavirus disease (COVID-19). Cell culture-based methods are the better way to perform viral isolation, evaluate viral infectivity, and amplify the virus. Furthermore, next-generation sequencing (NGS) have been essential to analyze a complete genome and to describe new viral species and lineages that have arisen over time. Four naso-oropharyngeal swab samples, collected from April to July of 2020, were isolated and sequenced aiming to produce viral stocks and analyze the mutational profile of the found lineage. B.1.1.33 was the lineage detected in all sequences. Although the samples belong to the same lineage, it was possible to evaluate different mutations found including some that were first described in these sequences, like the S:H655Y and T63N. The results described here can help to elicit how the pandemic started to spread and how it has been evolving in south Brazil.
Keywords: B.1.1.33; S:H655Y; S:T63N; SARS-CoV-2; Southern Brazil.