tetano
Editor, Senior Moderator
BMC Vet Res
. 2021 Feb 15;17(1):80.
doi: 10.1186/s12917-021-02786-0.
G1-like M and PB2 genes are preferentially incorporated into H7N9 progeny virions during genetic reassortment
Xiuli Li[SUP] 1 [/SUP], Min Gu[SUP] 1 [/SUP], Xiaoquan Wang[SUP] 1 [/SUP], Ruyi Gao[SUP] 1 [/SUP], Xinxin Bu[SUP] 1 [/SUP], Xiaoli Hao[SUP] 1 [/SUP], Jing Ma[SUP] 1 [/SUP], Jiao Hu[SUP] 1 [/SUP], Shunlin Hu[SUP] 1 [/SUP], Xiaowen Liu[SUP] 1 [/SUP], Sujuan Chen[SUP] 1 [/SUP], Daxin Peng[SUP] 1 [/SUP], Xinan Jiao[SUP] 2 3 4 [/SUP], Xiufan Liu[SUP] 5 6 7 8 [/SUP]
Affiliations
Abstract
Background: Genotype S H9N2 viruses have become predominant in poultry in China since 2010. These viruses frequently donate their whole internal gene segments to other emerging influenza A subtypes such as the novel H7N9, H5N6, and H10N8 viruses. We recently reported that the PB2 and M genes of the genotype S H9N2 virus, which are derived from the G1-like virus, enhance the fitness of H5Nx and H7N9 avian influenza viruses in chickens and mice. However, whether the G1-like PB2 and M genes are preferentially incorporated into progeny virions during virus reassortment remains unclear; whether the G1-like PB2 and M genes from different subtypes are differentially incorporated into new virion progeny remains unknown.
Results: We conducted a reassortment experiment with the use of a H7N9 virus as the backbone and found that G1-like M/PB2 genes were preferentially incorporated in progeny virions over F/98-like M/PB2 genes. Importantly, the preference varied among G1-like M/PB2 genes of different subtypes. When competing with F/98-like M/PB2 genes during reassortment, both the M and PB2 genes from the H7N9 virus GD15 showed an advantage, whereas only the PB2 gene from the H9N2 virus CZ73 and the M gene from the H9N2 virus AH320 displayed the advantage.
Conclusion: Our findings highlight the preferential and variable advantages of H9N2-derived G1-like M and PB2 genes in incorporating them into H7N9 progeny virions over SH14-derived F/98-like M/PB2 genes.
Keywords: Advantage; F/98-like M; F/98-like PB2; G1-like M; G1-like PB2; H7N9; H9N2; Reassortment.
. 2021 Feb 15;17(1):80.
doi: 10.1186/s12917-021-02786-0.
G1-like M and PB2 genes are preferentially incorporated into H7N9 progeny virions during genetic reassortment
Xiuli Li[SUP] 1 [/SUP], Min Gu[SUP] 1 [/SUP], Xiaoquan Wang[SUP] 1 [/SUP], Ruyi Gao[SUP] 1 [/SUP], Xinxin Bu[SUP] 1 [/SUP], Xiaoli Hao[SUP] 1 [/SUP], Jing Ma[SUP] 1 [/SUP], Jiao Hu[SUP] 1 [/SUP], Shunlin Hu[SUP] 1 [/SUP], Xiaowen Liu[SUP] 1 [/SUP], Sujuan Chen[SUP] 1 [/SUP], Daxin Peng[SUP] 1 [/SUP], Xinan Jiao[SUP] 2 3 4 [/SUP], Xiufan Liu[SUP] 5 6 7 8 [/SUP]
Affiliations
- PMID: 33588843
- DOI: 10.1186/s12917-021-02786-0
Abstract
Background: Genotype S H9N2 viruses have become predominant in poultry in China since 2010. These viruses frequently donate their whole internal gene segments to other emerging influenza A subtypes such as the novel H7N9, H5N6, and H10N8 viruses. We recently reported that the PB2 and M genes of the genotype S H9N2 virus, which are derived from the G1-like virus, enhance the fitness of H5Nx and H7N9 avian influenza viruses in chickens and mice. However, whether the G1-like PB2 and M genes are preferentially incorporated into progeny virions during virus reassortment remains unclear; whether the G1-like PB2 and M genes from different subtypes are differentially incorporated into new virion progeny remains unknown.
Results: We conducted a reassortment experiment with the use of a H7N9 virus as the backbone and found that G1-like M/PB2 genes were preferentially incorporated in progeny virions over F/98-like M/PB2 genes. Importantly, the preference varied among G1-like M/PB2 genes of different subtypes. When competing with F/98-like M/PB2 genes during reassortment, both the M and PB2 genes from the H7N9 virus GD15 showed an advantage, whereas only the PB2 gene from the H9N2 virus CZ73 and the M gene from the H9N2 virus AH320 displayed the advantage.
Conclusion: Our findings highlight the preferential and variable advantages of H9N2-derived G1-like M and PB2 genes in incorporating them into H7N9 progeny virions over SH14-derived F/98-like M/PB2 genes.
Keywords: Advantage; F/98-like M; F/98-like PB2; G1-like M; G1-like PB2; H7N9; H9N2; Reassortment.