tetano
Editor, Senior Moderator
BMC Med
. 2021 Oct 1;19(1):255.
doi: 10.1186/s12916-021-02103-4.
Transmission event of SARS-CoV-2 delta variant reveals multiple vaccine breakthrough infections
Timothy Farinholt[SUP] 1 [/SUP], Harsha Doddapaneni[SUP] 2 3 [/SUP], Xiang Qin[SUP] 2 3 [/SUP], Vipin Menon[SUP] 2 3 [/SUP], Qingchang Meng[SUP] 2 3 [/SUP], Ginger Metcalf[SUP] 2 3 [/SUP], Hsu Chao[SUP] 2 3 [/SUP], Marie-Claude Gingras[SUP] 2 3 [/SUP], Vasanthi Avadhanula[SUP] 4 [/SUP], Paige Farinholt[SUP] 5 [/SUP], Charu Agrawal[SUP] 5 [/SUP], Donna M Muzny[SUP] 2 3 [/SUP], Pedro A Piedra[SUP] 4 [/SUP], Richard A Gibbs[SUP] 2 3 [/SUP], Joseph Petrosino[SUP] 4 [/SUP]
Affiliations
Abstract
Background: This study aims to identify the causative strain of SARS-CoV-2 in a cluster of vaccine breakthroughs. Vaccine breakthrough by a highly transmissible SARS-CoV-2 strain is a risk to global public health.
Methods: Nasopharyngeal swabs from suspected vaccine breakthrough cases were tested for SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) by qPCR (quantitative polymerase chain reaction) for Wuhan-Hu1 and alpha variant. Positive samples were then sequenced by Swift Normalase Amplicon Panels to determine the causal variant. GATK (genome analysis toolkit) variants were filtered with allele fraction ≥80 and min read depth 30x.
Results: Viral sequencing revealed an infection cluster of 6 vaccinated patients infected with the delta (B.1.617.2) SARS-CoV-2 variant. With no history of vaccine breakthrough, this suggests the delta variant may possess immune evasion in patients that received the Pfizer BNT162b2, Moderna mRNA-1273, and Covaxin BBV152.
Conclusions: Delta variant may pose the highest risk out of any currently circulating SARS-CoV-2 variants, with previously described increased transmissibility over alpha variant and now, possible vaccine breakthrough.
Funding: Parts of this work was supported by the National Institute of Allergy and Infectious Diseases (1U19AI144297) and Baylor College of Medicine internal funding.
Keywords: B.1.617.2; COVID-19; Delta variant; Infectious disease; SARS-CoV-2.
. 2021 Oct 1;19(1):255.
doi: 10.1186/s12916-021-02103-4.
Transmission event of SARS-CoV-2 delta variant reveals multiple vaccine breakthrough infections
Timothy Farinholt[SUP] 1 [/SUP], Harsha Doddapaneni[SUP] 2 3 [/SUP], Xiang Qin[SUP] 2 3 [/SUP], Vipin Menon[SUP] 2 3 [/SUP], Qingchang Meng[SUP] 2 3 [/SUP], Ginger Metcalf[SUP] 2 3 [/SUP], Hsu Chao[SUP] 2 3 [/SUP], Marie-Claude Gingras[SUP] 2 3 [/SUP], Vasanthi Avadhanula[SUP] 4 [/SUP], Paige Farinholt[SUP] 5 [/SUP], Charu Agrawal[SUP] 5 [/SUP], Donna M Muzny[SUP] 2 3 [/SUP], Pedro A Piedra[SUP] 4 [/SUP], Richard A Gibbs[SUP] 2 3 [/SUP], Joseph Petrosino[SUP] 4 [/SUP]
Affiliations
- PMID: 34593004
- DOI: 10.1186/s12916-021-02103-4
Abstract
Background: This study aims to identify the causative strain of SARS-CoV-2 in a cluster of vaccine breakthroughs. Vaccine breakthrough by a highly transmissible SARS-CoV-2 strain is a risk to global public health.
Methods: Nasopharyngeal swabs from suspected vaccine breakthrough cases were tested for SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) by qPCR (quantitative polymerase chain reaction) for Wuhan-Hu1 and alpha variant. Positive samples were then sequenced by Swift Normalase Amplicon Panels to determine the causal variant. GATK (genome analysis toolkit) variants were filtered with allele fraction ≥80 and min read depth 30x.
Results: Viral sequencing revealed an infection cluster of 6 vaccinated patients infected with the delta (B.1.617.2) SARS-CoV-2 variant. With no history of vaccine breakthrough, this suggests the delta variant may possess immune evasion in patients that received the Pfizer BNT162b2, Moderna mRNA-1273, and Covaxin BBV152.
Conclusions: Delta variant may pose the highest risk out of any currently circulating SARS-CoV-2 variants, with previously described increased transmissibility over alpha variant and now, possible vaccine breakthrough.
Funding: Parts of this work was supported by the National Institute of Allergy and Infectious Diseases (1U19AI144297) and Baylor College of Medicine internal funding.
Keywords: B.1.617.2; COVID-19; Delta variant; Infectious disease; SARS-CoV-2.