tetano
Editor, Senior Moderator
BMC Infect Dis
. 2021 Nov 17;21(1):1162.
doi: 10.1186/s12879-021-06876-0.
Two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2
Meng Yee Lai[SUP] 1 [/SUP], Fatma Diyana Mohd Bukhari[SUP] 1 [/SUP], Nur Zulaikha Zulkefli[SUP] 1 [/SUP], Ilyiana Ismail[SUP] 2 [/SUP], Nur Izati Mustapa[SUP] 2 [/SUP], Tuan Suhaila Tuan Soh[SUP] 2 [/SUP], Afifah Haji Hassan[SUP] 2 [/SUP], Kalaiarasu M Peariasamy[SUP] 3 [/SUP], Yee Leng Lee[SUP] 4 [/SUP], Jeyanthi Suppiah[SUP] 5 [/SUP], Ravindran Thayan[SUP] 5 [/SUP], Yee Ling Lau[SUP] 6 [/SUP]
Affiliations
Abstract
Background: Current assays for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rely on time consuming, costly and laboratory based methods for virus isolation, purification and removing inhibitors. To address this limitation, we propose a simple method for testing RNA from nasopharyngeal swab samples that bypasses the RNA purification step.
Methods: In the current project, we have described two extraction-free reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays for the detection of SARS-CoV-2 by using E gene and RdRp gene as the targets.
Results: Here, results showed that reverse transcription loop-mediated isothermal amplification assays with 88.4% sensitive (95% CI: 74.9-96.1%) and 67.4% sensitive (95% CI: 51.5-80.9%) for E gene and RdRp gene, respectively.
Conclusion: Without the need of RNA purification, our developed RT-LAMP assays for direct detection of SARS-CoV-2 from nasopharyngeal swab samples could be turned into alternatives to qRT-PCR for rapid screening.
Keywords: Coronaviruses; Isothermal detection; RT-LAMP; Rapid diagnosis; SARS-CoV-2.
. 2021 Nov 17;21(1):1162.
doi: 10.1186/s12879-021-06876-0.
Two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2
Meng Yee Lai[SUP] 1 [/SUP], Fatma Diyana Mohd Bukhari[SUP] 1 [/SUP], Nur Zulaikha Zulkefli[SUP] 1 [/SUP], Ilyiana Ismail[SUP] 2 [/SUP], Nur Izati Mustapa[SUP] 2 [/SUP], Tuan Suhaila Tuan Soh[SUP] 2 [/SUP], Afifah Haji Hassan[SUP] 2 [/SUP], Kalaiarasu M Peariasamy[SUP] 3 [/SUP], Yee Leng Lee[SUP] 4 [/SUP], Jeyanthi Suppiah[SUP] 5 [/SUP], Ravindran Thayan[SUP] 5 [/SUP], Yee Ling Lau[SUP] 6 [/SUP]
Affiliations
- PMID: 34789179
- DOI: 10.1186/s12879-021-06876-0
Abstract
Background: Current assays for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rely on time consuming, costly and laboratory based methods for virus isolation, purification and removing inhibitors. To address this limitation, we propose a simple method for testing RNA from nasopharyngeal swab samples that bypasses the RNA purification step.
Methods: In the current project, we have described two extraction-free reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays for the detection of SARS-CoV-2 by using E gene and RdRp gene as the targets.
Results: Here, results showed that reverse transcription loop-mediated isothermal amplification assays with 88.4% sensitive (95% CI: 74.9-96.1%) and 67.4% sensitive (95% CI: 51.5-80.9%) for E gene and RdRp gene, respectively.
Conclusion: Without the need of RNA purification, our developed RT-LAMP assays for direct detection of SARS-CoV-2 from nasopharyngeal swab samples could be turned into alternatives to qRT-PCR for rapid screening.
Keywords: Coronaviruses; Isothermal detection; RT-LAMP; Rapid diagnosis; SARS-CoV-2.