tetano
Editor, Senior Moderator
BMC Infect Dis
. 2022 Jan 4;22(1):10.
doi: 10.1186/s12879-021-07010-w.
Surveillance of SARS-CoV-2 antibodies of patients in the local affected area during Wuhan lockdown
Yueting Tang[SUP] #[/SUP][SUP] 1 [/SUP], Jiayu Sun[SUP] #[/SUP][SUP] 2 [/SUP], Yumeng Yuan[SUP] 2 [/SUP], Fen Yao[SUP] 2 [/SUP], Bokun Zheng[SUP] 1 [/SUP], Gui Yang[SUP] 1 [/SUP], Wen Xie[SUP] 1 [/SUP], Guangming Ye[SUP] 3 [/SUP], Zhen Li[SUP] 1 [/SUP], Xiaoyang Jiao[SUP] 4 [/SUP], Yirong Li[SUP] 5 [/SUP]
Affiliations
Abstract
Background: Serosurveillance is crucial in estimating the range of SARS-CoV-2 infections, predicting the possibility of another wave, and deciding on a vaccination strategy. To understand the herd immunity after the COVID-19 pandemic, the seroprevalence was measured in 3062 individuals with or without COVID-19 from the clinic.
Methods: The levels of SARS-CoV-2 antibody IgM and IgG were measured by the immuno-colloidal gold method. A fusion fragment of nucleocapsid and spike protein was detected by a qualitative test kit with sensitivity (89%) and specificity (98%).
Results: The seroprevalence rate for IgM and IgG in all outpatients was 2.81% and 7.51%, respectively. The sex-related prevalence rate of IgG was significantly higher (P < 0.05) in women than men. The highest positive rate of IgM was observed in individuals < 20 years of age (3.57%), while the highest seroprevalence for IgG was observed in persons > 60 years of age (8.61%). Positive rates of IgM and IgG in the convalescent patients were 31.82% and 77.27%, respectively, which was significantly higher than individuals with suspected syndromes or individuals without any clinical signs (P < 0.01). Seroprevalence for IgG in medical staff was markedly higher than those in residents. No significant difference of seroprevalence was found among patients with different comorbidities (P > 0.05).
Conclusions: The low positive rate of the SARS-CoV-2 IgM and nucleic acid (NA) test indicated that the SARS-CoV-2 outbreak is subsiding after 3 months, and the possibility of reintroduction of the virus from an unidentified natural reservoir is low. Seroprevalence provides information for humoral immunity and vaccine in the future.
Keywords: COVID-19; SARS-CoV-2; Serosurveillance.
. 2022 Jan 4;22(1):10.
doi: 10.1186/s12879-021-07010-w.
Surveillance of SARS-CoV-2 antibodies of patients in the local affected area during Wuhan lockdown
Yueting Tang[SUP] #[/SUP][SUP] 1 [/SUP], Jiayu Sun[SUP] #[/SUP][SUP] 2 [/SUP], Yumeng Yuan[SUP] 2 [/SUP], Fen Yao[SUP] 2 [/SUP], Bokun Zheng[SUP] 1 [/SUP], Gui Yang[SUP] 1 [/SUP], Wen Xie[SUP] 1 [/SUP], Guangming Ye[SUP] 3 [/SUP], Zhen Li[SUP] 1 [/SUP], Xiaoyang Jiao[SUP] 4 [/SUP], Yirong Li[SUP] 5 [/SUP]
Affiliations
- PMID: 34983429
- DOI: 10.1186/s12879-021-07010-w
Abstract
Background: Serosurveillance is crucial in estimating the range of SARS-CoV-2 infections, predicting the possibility of another wave, and deciding on a vaccination strategy. To understand the herd immunity after the COVID-19 pandemic, the seroprevalence was measured in 3062 individuals with or without COVID-19 from the clinic.
Methods: The levels of SARS-CoV-2 antibody IgM and IgG were measured by the immuno-colloidal gold method. A fusion fragment of nucleocapsid and spike protein was detected by a qualitative test kit with sensitivity (89%) and specificity (98%).
Results: The seroprevalence rate for IgM and IgG in all outpatients was 2.81% and 7.51%, respectively. The sex-related prevalence rate of IgG was significantly higher (P < 0.05) in women than men. The highest positive rate of IgM was observed in individuals < 20 years of age (3.57%), while the highest seroprevalence for IgG was observed in persons > 60 years of age (8.61%). Positive rates of IgM and IgG in the convalescent patients were 31.82% and 77.27%, respectively, which was significantly higher than individuals with suspected syndromes or individuals without any clinical signs (P < 0.01). Seroprevalence for IgG in medical staff was markedly higher than those in residents. No significant difference of seroprevalence was found among patients with different comorbidities (P > 0.05).
Conclusions: The low positive rate of the SARS-CoV-2 IgM and nucleic acid (NA) test indicated that the SARS-CoV-2 outbreak is subsiding after 3 months, and the possibility of reintroduction of the virus from an unidentified natural reservoir is low. Seroprevalence provides information for humoral immunity and vaccine in the future.
Keywords: COVID-19; SARS-CoV-2; Serosurveillance.