tetano
Editor, Senior Moderator
BMC Infect Dis
. 2021 May 4;21(1):410.
doi: 10.1186/s12879-021-06108-5.
Salivary testing of COVID-19: evaluation of serological testing following positive salivary results
Lisa Caulley[SUP] 1 [/SUP], Julie Shaw[SUP] 2 [/SUP], Martin Corsten[SUP] 3 [/SUP], Nadia Hua[SUP] 1 [/SUP], Jonathan B Angel[SUP] 4 5 [/SUP], Guillaume Poliquin[SUP] 6 7 [/SUP], Jonathan Whelan[SUP] 8 [/SUP], Kym Antonation[SUP] 6 [/SUP], Stephanie Johnson-Obaseki[SUP] 9 [/SUP]
Affiliations
Abstract
Background: Salivary detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been proposed as an alternative to nasopharyngeal or oropharyngeal swab testing. Our group previously published a study demonstrating that both testing methods identified SARS-CoV-2 using polymerase chain reaction (PCR)-based detection methodology. We therefore conducted a follow-up study using antibody testing to evaluate the accuracy of saliva versus swabs for COVID-19 detection and the durability of antibody response.
Methods: Venous blood samples were collected from consenting participants and the presence of serum antibodies for SARS-CoV-2 was evaluated on a large, automated immunoassay platform by the Roche anti-SARS-CoV-2 qualitative assay (Roche Diagnostics, Laval Quebec). Individuals with a serum antibody cut-off index (COI) ? 1.0 were considered positive.
Results: In asymptomatic and mildly symptomatic patients with a previously positive standard swab and/or saliva SARS-CoV-2 PCR-test, 42 demonstrated antibodies with 13 patients positive by swab alone, and 8 patients positive by saliva alone.
Conclusions: Despite their status as 'current standard' for COVID-19 testing, these findings highlight limitations of PCR-based tests.
Keywords: Antibodies; COVID-19; Coronavirus; SARS-CoV-2; Saliva.
. 2021 May 4;21(1):410.
doi: 10.1186/s12879-021-06108-5.
Salivary testing of COVID-19: evaluation of serological testing following positive salivary results
Lisa Caulley[SUP] 1 [/SUP], Julie Shaw[SUP] 2 [/SUP], Martin Corsten[SUP] 3 [/SUP], Nadia Hua[SUP] 1 [/SUP], Jonathan B Angel[SUP] 4 5 [/SUP], Guillaume Poliquin[SUP] 6 7 [/SUP], Jonathan Whelan[SUP] 8 [/SUP], Kym Antonation[SUP] 6 [/SUP], Stephanie Johnson-Obaseki[SUP] 9 [/SUP]
Affiliations
- PMID: 33947347
- DOI: 10.1186/s12879-021-06108-5
Abstract
Background: Salivary detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been proposed as an alternative to nasopharyngeal or oropharyngeal swab testing. Our group previously published a study demonstrating that both testing methods identified SARS-CoV-2 using polymerase chain reaction (PCR)-based detection methodology. We therefore conducted a follow-up study using antibody testing to evaluate the accuracy of saliva versus swabs for COVID-19 detection and the durability of antibody response.
Methods: Venous blood samples were collected from consenting participants and the presence of serum antibodies for SARS-CoV-2 was evaluated on a large, automated immunoassay platform by the Roche anti-SARS-CoV-2 qualitative assay (Roche Diagnostics, Laval Quebec). Individuals with a serum antibody cut-off index (COI) ? 1.0 were considered positive.
Results: In asymptomatic and mildly symptomatic patients with a previously positive standard swab and/or saliva SARS-CoV-2 PCR-test, 42 demonstrated antibodies with 13 patients positive by swab alone, and 8 patients positive by saliva alone.
Conclusions: Despite their status as 'current standard' for COVID-19 testing, these findings highlight limitations of PCR-based tests.
Keywords: Antibodies; COVID-19; Coronavirus; SARS-CoV-2; Saliva.