tetano
Editor, Senior Moderator
BMC Infect Dis
. 2024 Jun 6;24(1):567.
doi: 10.1186/s12879-024-09464-0. Longitudinal dynamic single-cell mass cytometry analysis of peripheral blood mononuclear cells in COVID-19 patients within 6 months after viral RNA clearance
Diwenxin Zhou[SUP] 1 [/SUP], Shuai Zhao[SUP] 1 [/SUP], Keting He[SUP] 1 [/SUP], Qiuhong Liu[SUP] 1 [/SUP], Fen Zhang[SUP] 1 [/SUP], Zhangya Pu[SUP] 1 [/SUP], Lanlan Xiao[SUP] 1 [/SUP], Lingjian Zhang[SUP] 1 [/SUP], Shangci Chen[SUP] 1 [/SUP], Xiaohan Qian[SUP] 1 [/SUP], Xiaoxin Wu[SUP] 1 [/SUP], Yangfan Shen[SUP] 1 [/SUP], Ling Yu[SUP] 1 [/SUP], Huafen Zhang[SUP] 1 [/SUP], Jiandi Jin[SUP] 1 [/SUP], Min Xu[SUP] 1 [/SUP], Xiaoyan Wang[SUP] 1 [/SUP], Danhua Zhu[SUP] 1 [/SUP], Zhongyang Xie[SUP] 2 [/SUP], Xiaowei Xu[SUP] 3 [/SUP]
Affiliations
This study investigates the longitudinal dynamic changes in immune cells in COVID-19 patients over an extended period after recovery, as well as the interplay between immune cells and antibodies. Leveraging single-cell mass spectrometry, we selected six COVID-19 patients and four healthy controls, dissecting the evolving landscape within six months post-viral RNA clearance, alongside the levels of anti-spike protein antibodies. The T cell immunophenotype ascertained via single-cell mass spectrometry underwent validation through flow cytometry in 37 samples. Our findings illuminate that CD8 + T cells, gamma-delta (gd) T cells, and NK cells witnessed an increase, in contrast to the reduction observed in monocytes, B cells, and double-negative T (DNT) cells over time. The proportion of monocytes remained significantly elevated in COVID-19 patients compared to controls even after six-month. Subpopulation-wise, an upsurge manifested within various T effector memory subsets, CD45RA + T effector memory, gdT, and NK cells, whereas declines marked the populations of DNT, naive and memory B cells, and classical as well as non-classical monocytes. Noteworthy associations surfaced between DNT, gdT, CD4 + T, NK cells, and the anti-S antibody titer. This study reveals the changes in peripheral blood mononuclear cells of COVID-19 patients within 6 months after viral RNA clearance and sheds light on the interactions between immune cells and antibodies. The findings from this research contribute to a better understanding of immune transformations during the recovery from COVID-19 and offer guidance for protective measures against reinfection in the context of viral variants.
Keywords: COVID-19; CyTOF; Dynamics; PBMC (peripheral blood mononuclear cells); Recovery.
. 2024 Jun 6;24(1):567.
doi: 10.1186/s12879-024-09464-0. Longitudinal dynamic single-cell mass cytometry analysis of peripheral blood mononuclear cells in COVID-19 patients within 6 months after viral RNA clearance
Diwenxin Zhou[SUP] 1 [/SUP], Shuai Zhao[SUP] 1 [/SUP], Keting He[SUP] 1 [/SUP], Qiuhong Liu[SUP] 1 [/SUP], Fen Zhang[SUP] 1 [/SUP], Zhangya Pu[SUP] 1 [/SUP], Lanlan Xiao[SUP] 1 [/SUP], Lingjian Zhang[SUP] 1 [/SUP], Shangci Chen[SUP] 1 [/SUP], Xiaohan Qian[SUP] 1 [/SUP], Xiaoxin Wu[SUP] 1 [/SUP], Yangfan Shen[SUP] 1 [/SUP], Ling Yu[SUP] 1 [/SUP], Huafen Zhang[SUP] 1 [/SUP], Jiandi Jin[SUP] 1 [/SUP], Min Xu[SUP] 1 [/SUP], Xiaoyan Wang[SUP] 1 [/SUP], Danhua Zhu[SUP] 1 [/SUP], Zhongyang Xie[SUP] 2 [/SUP], Xiaowei Xu[SUP] 3 [/SUP]
Affiliations
- PMID: 38844850
- DOI: 10.1186/s12879-024-09464-0
This study investigates the longitudinal dynamic changes in immune cells in COVID-19 patients over an extended period after recovery, as well as the interplay between immune cells and antibodies. Leveraging single-cell mass spectrometry, we selected six COVID-19 patients and four healthy controls, dissecting the evolving landscape within six months post-viral RNA clearance, alongside the levels of anti-spike protein antibodies. The T cell immunophenotype ascertained via single-cell mass spectrometry underwent validation through flow cytometry in 37 samples. Our findings illuminate that CD8 + T cells, gamma-delta (gd) T cells, and NK cells witnessed an increase, in contrast to the reduction observed in monocytes, B cells, and double-negative T (DNT) cells over time. The proportion of monocytes remained significantly elevated in COVID-19 patients compared to controls even after six-month. Subpopulation-wise, an upsurge manifested within various T effector memory subsets, CD45RA + T effector memory, gdT, and NK cells, whereas declines marked the populations of DNT, naive and memory B cells, and classical as well as non-classical monocytes. Noteworthy associations surfaced between DNT, gdT, CD4 + T, NK cells, and the anti-S antibody titer. This study reveals the changes in peripheral blood mononuclear cells of COVID-19 patients within 6 months after viral RNA clearance and sheds light on the interactions between immune cells and antibodies. The findings from this research contribute to a better understanding of immune transformations during the recovery from COVID-19 and offer guidance for protective measures against reinfection in the context of viral variants.
Keywords: COVID-19; CyTOF; Dynamics; PBMC (peripheral blood mononuclear cells); Recovery.