tetano
Editor, Senior Moderator
BMC Immunol
. 2022 Apr 25;23(1):21.
doi: 10.1186/s12865-022-00494-4.
Influenza virus-like particle vaccine containing both apical membrane antigen 1 and microneme-associated antigen proteins of Plasmodium berghei confers protection in mice
Min-Ju Kim[SUP] 1 [/SUP], Ki-Back Chu[SUP] 1 [/SUP], Hae-Ji Kang[SUP] 1 [/SUP], Keon-Woong Yoon[SUP] 1 [/SUP], Dong-Hun Lee[SUP] 1 [/SUP], Su-Hwa Lee[SUP] 1 [/SUP], Eun-Kyung Moon[SUP] 2 [/SUP], Fu-Shi Quan[SUP] 3 4 [/SUP]
Affiliations
Abstract
Background: Apical membrane antigen 1 (AMA1) and microneme-associated antigen (MIC) of Plasmodium parasites are important factors involved in host cell invasion.
Methods: In this study, influenza VLP vaccines containing both codon-optimized AMA1 and MIC were generated and the vaccine efficacy was evaluated in mice.
Results: VLPs vaccine immunization elicited higher levels of parasite-specific IgG and IgG2a antibody responses in sera. CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T cells and germinal center B cells in blood, inguinal lymph nodes (ILN) and spleen were found to be significantly increased. Importantly, VLPs vaccination significantly reduced the levels of pro-inflammatory cytokines IFN-γ and TNF-α, decreased parasitemia in blood, resulting in lower body weight loss and longer survival time compared to control.
Conclusion: These results indicated that VLPs containing P. berghei AMA1 and MIC could be a candidate for malaria blood-stage vaccine design.
Keywords: Apical membrane antigen 1; Microneme; Plasmodium berghei; Vaccine; Virus-like particles.
. 2022 Apr 25;23(1):21.
doi: 10.1186/s12865-022-00494-4.
Influenza virus-like particle vaccine containing both apical membrane antigen 1 and microneme-associated antigen proteins of Plasmodium berghei confers protection in mice
Min-Ju Kim[SUP] 1 [/SUP], Ki-Back Chu[SUP] 1 [/SUP], Hae-Ji Kang[SUP] 1 [/SUP], Keon-Woong Yoon[SUP] 1 [/SUP], Dong-Hun Lee[SUP] 1 [/SUP], Su-Hwa Lee[SUP] 1 [/SUP], Eun-Kyung Moon[SUP] 2 [/SUP], Fu-Shi Quan[SUP] 3 4 [/SUP]
Affiliations
- PMID: 35468726
- DOI: 10.1186/s12865-022-00494-4
Abstract
Background: Apical membrane antigen 1 (AMA1) and microneme-associated antigen (MIC) of Plasmodium parasites are important factors involved in host cell invasion.
Methods: In this study, influenza VLP vaccines containing both codon-optimized AMA1 and MIC were generated and the vaccine efficacy was evaluated in mice.
Results: VLPs vaccine immunization elicited higher levels of parasite-specific IgG and IgG2a antibody responses in sera. CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T cells and germinal center B cells in blood, inguinal lymph nodes (ILN) and spleen were found to be significantly increased. Importantly, VLPs vaccination significantly reduced the levels of pro-inflammatory cytokines IFN-γ and TNF-α, decreased parasitemia in blood, resulting in lower body weight loss and longer survival time compared to control.
Conclusion: These results indicated that VLPs containing P. berghei AMA1 and MIC could be a candidate for malaria blood-stage vaccine design.
Keywords: Apical membrane antigen 1; Microneme; Plasmodium berghei; Vaccine; Virus-like particles.