tetano
Editor, Senior Moderator
Biosensors (Basel)
. 2021 Dec 29;12(1):13.
doi: 10.3390/bios12010013.
Lateral Flow Immunoassay Coupled with Copper Enhancement for Rapid and Sensitive SARS-CoV-2 Nucleocapsid Protein Detection
Tao Peng[SUP] 1 [/SUP], Xueshima Jiao[SUP] 1 2 [/SUP], Zhanwei Liang[SUP] 1 2 [/SUP], Hongwei Zhao[SUP] 3 [/SUP], Yang Zhao[SUP] 1 [/SUP], Jie Xie[SUP] 1 [/SUP], You Jiang[SUP] 1 [/SUP], Xiaoping Yu[SUP] 2 [/SUP], Xiang Fang[SUP] 1 [/SUP], Xinhua Dai[SUP] 1 [/SUP]
Affiliations
Abstract
The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory coronavirus 2 (SARS-CoV-2) is still raging all over the world. Hence, the rapid and sensitive screening of the suspected population is in high demand. The nucleocapsid protein (NP) of SARS-CoV-2 has been selected as an ideal marker for viral antigen detection. This study describes a lateral flow immunoassay (LFIA) based on colloidal gold nanoparticles for rapid NP antigen detection, in which sensitivity was improved through copper deposition-induced signal amplification. The detection sensitivity of the developed LFIA for NP antigen detection (using certified reference materials) under the optimized parameters was 0.01 μg/mL and was promoted by three orders of magnitude to 10 pg/mL after copper deposition signal amplification. The LFIA coupled with the copper enhancement technique has many merits such as low cost, high efficiency, and high sensitivity. It provides an effective approach to the rapid screening, diagnosis, and monitoring of the suspected population in the COVID-19 outbreak.
Keywords: SARS-CoV-2; copper deposition; nucleocapsid protein; signal amplification.
. 2021 Dec 29;12(1):13.
doi: 10.3390/bios12010013.
Lateral Flow Immunoassay Coupled with Copper Enhancement for Rapid and Sensitive SARS-CoV-2 Nucleocapsid Protein Detection
Tao Peng[SUP] 1 [/SUP], Xueshima Jiao[SUP] 1 2 [/SUP], Zhanwei Liang[SUP] 1 2 [/SUP], Hongwei Zhao[SUP] 3 [/SUP], Yang Zhao[SUP] 1 [/SUP], Jie Xie[SUP] 1 [/SUP], You Jiang[SUP] 1 [/SUP], Xiaoping Yu[SUP] 2 [/SUP], Xiang Fang[SUP] 1 [/SUP], Xinhua Dai[SUP] 1 [/SUP]
Affiliations
- PMID: 35049641
- DOI: 10.3390/bios12010013
Abstract
The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory coronavirus 2 (SARS-CoV-2) is still raging all over the world. Hence, the rapid and sensitive screening of the suspected population is in high demand. The nucleocapsid protein (NP) of SARS-CoV-2 has been selected as an ideal marker for viral antigen detection. This study describes a lateral flow immunoassay (LFIA) based on colloidal gold nanoparticles for rapid NP antigen detection, in which sensitivity was improved through copper deposition-induced signal amplification. The detection sensitivity of the developed LFIA for NP antigen detection (using certified reference materials) under the optimized parameters was 0.01 μg/mL and was promoted by three orders of magnitude to 10 pg/mL after copper deposition signal amplification. The LFIA coupled with the copper enhancement technique has many merits such as low cost, high efficiency, and high sensitivity. It provides an effective approach to the rapid screening, diagnosis, and monitoring of the suspected population in the COVID-19 outbreak.
Keywords: SARS-CoV-2; copper deposition; nucleocapsid protein; signal amplification.