tetano
Editor, Senior Moderator
Biosens Bioelectron
. 2022 Jan 20;202:114008.
doi: 10.1016/j.bios.2022.114008. Online ahead of print.
Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2
Hyunjung Cha[SUP] 1 [/SUP], Hyeran Kim[SUP] 1 [/SUP], Younju Joung[SUP] 2 [/SUP], Hyunju Kang[SUP] 1 [/SUP], Jeong Moon[SUP] 3 [/SUP], Hyowon Jang[SUP] 1 [/SUP], Sohyun Park[SUP] 2 [/SUP], Hyung-Jun Kwon[SUP] 4 [/SUP], In-Chul Lee[SUP] 4 [/SUP], Sunjoo Kim[SUP] 5 [/SUP], Dongeun Yong[SUP] 6 [/SUP], Sun-Woo Yoon[SUP] 1 [/SUP], Sung-Gyu Park[SUP] 7 [/SUP], Kyeonghye Guk[SUP] 1 [/SUP], Eun-Kyung Lim[SUP] 8 [/SUP], Hyun Gyu Park[SUP] 9 [/SUP], Jaebum Choo[SUP] 10 [/SUP], Juyeon Jung[SUP] 11 [/SUP], Taejoon Kang[SUP] 12 [/SUP]
Affiliations
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected humans worldwide for over a year now. Although various tests have been developed for the detection of SARS-CoV-2, advanced sensing methods are required for the diagnosis, screening, and surveillance of coronavirus disease 2019 (COVID-19). Here, we report a surface-enhanced Raman scattering (SERS)-based immunoassay involving an antibody pair, SERS-active hollow Au nanoparticles (NPs), and magnetic beads for the detection of SARS-CoV-2. The selected antibody pair against the SARS-CoV-2 antigen, along with the magnetic beads, facilitates the accurate direct detection of the virus. The hollow Au NPs exhibit strong, reproducible SERS signals, allowing sensitive quantitative detection of SARS-CoV-2. This assay had detection limits of 2.56 fg/mL for the SARS-CoV-2 antigen and 3.4 plaque-forming units/mL for the SARS-CoV-2 lysates. Furthermore, it facilitated the identification of SARS-CoV-2 in human nasopharyngeal aspirates and diagnosis of COVID-19 within 30 min using a portable Raman device. Thus, this assay can be potentially used for the diagnosis and prevention of COVID-19.
Keywords: Antibody; Coronavirus disease 2019; Immunoassay; Nanoparticle; Severe acute respiratory syndrome coronavirus 2; Surface-enhanced Raman scattering.
. 2022 Jan 20;202:114008.
doi: 10.1016/j.bios.2022.114008. Online ahead of print.
Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2
Hyunjung Cha[SUP] 1 [/SUP], Hyeran Kim[SUP] 1 [/SUP], Younju Joung[SUP] 2 [/SUP], Hyunju Kang[SUP] 1 [/SUP], Jeong Moon[SUP] 3 [/SUP], Hyowon Jang[SUP] 1 [/SUP], Sohyun Park[SUP] 2 [/SUP], Hyung-Jun Kwon[SUP] 4 [/SUP], In-Chul Lee[SUP] 4 [/SUP], Sunjoo Kim[SUP] 5 [/SUP], Dongeun Yong[SUP] 6 [/SUP], Sun-Woo Yoon[SUP] 1 [/SUP], Sung-Gyu Park[SUP] 7 [/SUP], Kyeonghye Guk[SUP] 1 [/SUP], Eun-Kyung Lim[SUP] 8 [/SUP], Hyun Gyu Park[SUP] 9 [/SUP], Jaebum Choo[SUP] 10 [/SUP], Juyeon Jung[SUP] 11 [/SUP], Taejoon Kang[SUP] 12 [/SUP]
Affiliations
- PMID: 35086030
- DOI: 10.1016/j.bios.2022.114008
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected humans worldwide for over a year now. Although various tests have been developed for the detection of SARS-CoV-2, advanced sensing methods are required for the diagnosis, screening, and surveillance of coronavirus disease 2019 (COVID-19). Here, we report a surface-enhanced Raman scattering (SERS)-based immunoassay involving an antibody pair, SERS-active hollow Au nanoparticles (NPs), and magnetic beads for the detection of SARS-CoV-2. The selected antibody pair against the SARS-CoV-2 antigen, along with the magnetic beads, facilitates the accurate direct detection of the virus. The hollow Au NPs exhibit strong, reproducible SERS signals, allowing sensitive quantitative detection of SARS-CoV-2. This assay had detection limits of 2.56 fg/mL for the SARS-CoV-2 antigen and 3.4 plaque-forming units/mL for the SARS-CoV-2 lysates. Furthermore, it facilitated the identification of SARS-CoV-2 in human nasopharyngeal aspirates and diagnosis of COVID-19 within 30 min using a portable Raman device. Thus, this assay can be potentially used for the diagnosis and prevention of COVID-19.
Keywords: Antibody; Coronavirus disease 2019; Immunoassay; Nanoparticle; Severe acute respiratory syndrome coronavirus 2; Surface-enhanced Raman scattering.