tetano
Editor, Senior Moderator
Biosens Bioelectron
. 2022 Jan 10;202:113978.
doi: 10.1016/j.bios.2022.113978. Online ahead of print.
SARS-CoV-2 detection using quantum dot fluorescence immunochromatography combined with isothermal amplification and CRISPR/Cas13a
Qin Zhang[SUP] 1 [/SUP], Jiahao Li[SUP] 2 [/SUP], Yue Li[SUP] 2 [/SUP], Guolei Tan[SUP] 1 [/SUP], Mei Sun[SUP] 1 [/SUP], Yanke Shan[SUP] 2 [/SUP], Yue Zhang[SUP] 2 [/SUP], Xin Wang[SUP] 2 [/SUP], Keyu Song[SUP] 1 [/SUP], Rui Shi[SUP] 1 [/SUP], Ling Huang[SUP] 1 [/SUP], Fei Liu[SUP] 3 [/SUP], Yongxiang Yi[SUP] 4 [/SUP], Xuping Wu[SUP] 5 [/SUP]
Affiliations
Abstract
The development of reliable, sensitive, and fast devices for the diagnosis of COVID-19 is of great importance in the pandemic of the new coronavirus. Here, we proposed a new principle of analysis based on a combination of reverse transcription and isothermal amplification of a fragment of the gene encoding the S protein of the SARS-CoV-2 and the CRISPR/Cas13a reaction for cleavage of the specific probe. As a result, the destroyed probe cannot be detected on an immunochromatographic strip using quantum fluorescent dots. Besides, the results can be obtained by an available and inexpensive portable device. By detecting SARS-CoV-2 negative (n = 25) and positive (n = 62) clinical samples including throat swabs, sputum and anal swabs, the assay showed good sensitivity and specificity of the method and could be completed within 1 h without complicated operation and expensive equipment. These superiorities showed its potential for fast point-of-care screening of SARS-CoV-2 during the outbreak, especially in remote and underdeveloped areas with limited equipment and resources.
Keywords: CRISPR/Cas13a; Isothermal amplification; Quantum dot fluorescence immune-chromatography; SARS-CoV-2.
. 2022 Jan 10;202:113978.
doi: 10.1016/j.bios.2022.113978. Online ahead of print.
SARS-CoV-2 detection using quantum dot fluorescence immunochromatography combined with isothermal amplification and CRISPR/Cas13a
Qin Zhang[SUP] 1 [/SUP], Jiahao Li[SUP] 2 [/SUP], Yue Li[SUP] 2 [/SUP], Guolei Tan[SUP] 1 [/SUP], Mei Sun[SUP] 1 [/SUP], Yanke Shan[SUP] 2 [/SUP], Yue Zhang[SUP] 2 [/SUP], Xin Wang[SUP] 2 [/SUP], Keyu Song[SUP] 1 [/SUP], Rui Shi[SUP] 1 [/SUP], Ling Huang[SUP] 1 [/SUP], Fei Liu[SUP] 3 [/SUP], Yongxiang Yi[SUP] 4 [/SUP], Xuping Wu[SUP] 5 [/SUP]
Affiliations
- PMID: 35086029
- DOI: 10.1016/j.bios.2022.113978
Abstract
The development of reliable, sensitive, and fast devices for the diagnosis of COVID-19 is of great importance in the pandemic of the new coronavirus. Here, we proposed a new principle of analysis based on a combination of reverse transcription and isothermal amplification of a fragment of the gene encoding the S protein of the SARS-CoV-2 and the CRISPR/Cas13a reaction for cleavage of the specific probe. As a result, the destroyed probe cannot be detected on an immunochromatographic strip using quantum fluorescent dots. Besides, the results can be obtained by an available and inexpensive portable device. By detecting SARS-CoV-2 negative (n = 25) and positive (n = 62) clinical samples including throat swabs, sputum and anal swabs, the assay showed good sensitivity and specificity of the method and could be completed within 1 h without complicated operation and expensive equipment. These superiorities showed its potential for fast point-of-care screening of SARS-CoV-2 during the outbreak, especially in remote and underdeveloped areas with limited equipment and resources.
Keywords: CRISPR/Cas13a; Isothermal amplification; Quantum dot fluorescence immune-chromatography; SARS-CoV-2.