tetano
Editor, Senior Moderator
Biosens Bioelectron
. 2022 Apr 29;210:114331.
doi: 10.1016/j.bios.2022.114331. Online ahead of print.
Electronic field effect detection of SARS-CoV-2 N-protein before the onset of symptoms
I Novodchuk[SUP] 1 [/SUP], M Kayaharman[SUP] 2 [/SUP], I Prassas[SUP] 3 [/SUP], A Soosaipillai[SUP] 3 [/SUP], R Karimi[SUP] 4 [/SUP], I A Goldthorpe[SUP] 2 [/SUP], E Abdel-Rahman[SUP] 5 [/SUP], J Sanderson[SUP] 4 [/SUP], E P Diamandis[SUP] 6 [/SUP], M Bajcsy[SUP] 7 [/SUP], M Yavuz[SUP] 8 [/SUP]
Affiliations
Abstract
As part of the efforts to contain the pandemic, researchers around the world have raced to develop testing platforms to detect the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes the Coronavirus disease 2019 (COVID-19). Within the different detection platforms studied, the field effect transistor (FET) is a promising device due to its high sensitivity and fast detection capabilities. In this work, a graphene-based FET which uses a boron and nitrogen co-doped graphene oxide gel (BN-GO gel) transducer functionalized with nucleoprotein antibodies, has been investigated for the detection of SARS-CoV-2 nucleocapsid (N)-protein in buffer. This biosensor was able to detect the viral protein in less than 4 min, with a limit of detection (LOD) as low as 10 ag/mL and a wide linear detection range stretching over 11 orders of magnitude from 10 ag/mL-1 μg/mL. This represents the lowest LOD and widest detection range of any COVID-19 sensor and thus can potentially enable the detection of infected individuals before they become contagious. In addition to its potential use in the COVID-19 pandemic, our device serves as a proof-of-concept of the ability of functionalized BN-GO gel FETs to be used for ultrasensitive yet robust biosensors.
Keywords: Biosensor; Covid-19; FET; Graphene.
. 2022 Apr 29;210:114331.
doi: 10.1016/j.bios.2022.114331. Online ahead of print.
Electronic field effect detection of SARS-CoV-2 N-protein before the onset of symptoms
I Novodchuk[SUP] 1 [/SUP], M Kayaharman[SUP] 2 [/SUP], I Prassas[SUP] 3 [/SUP], A Soosaipillai[SUP] 3 [/SUP], R Karimi[SUP] 4 [/SUP], I A Goldthorpe[SUP] 2 [/SUP], E Abdel-Rahman[SUP] 5 [/SUP], J Sanderson[SUP] 4 [/SUP], E P Diamandis[SUP] 6 [/SUP], M Bajcsy[SUP] 7 [/SUP], M Yavuz[SUP] 8 [/SUP]
Affiliations
- PMID: 35512584
- DOI: 10.1016/j.bios.2022.114331
Abstract
As part of the efforts to contain the pandemic, researchers around the world have raced to develop testing platforms to detect the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes the Coronavirus disease 2019 (COVID-19). Within the different detection platforms studied, the field effect transistor (FET) is a promising device due to its high sensitivity and fast detection capabilities. In this work, a graphene-based FET which uses a boron and nitrogen co-doped graphene oxide gel (BN-GO gel) transducer functionalized with nucleoprotein antibodies, has been investigated for the detection of SARS-CoV-2 nucleocapsid (N)-protein in buffer. This biosensor was able to detect the viral protein in less than 4 min, with a limit of detection (LOD) as low as 10 ag/mL and a wide linear detection range stretching over 11 orders of magnitude from 10 ag/mL-1 μg/mL. This represents the lowest LOD and widest detection range of any COVID-19 sensor and thus can potentially enable the detection of infected individuals before they become contagious. In addition to its potential use in the COVID-19 pandemic, our device serves as a proof-of-concept of the ability of functionalized BN-GO gel FETs to be used for ultrasensitive yet robust biosensors.
Keywords: Biosensor; Covid-19; FET; Graphene.