Mary Wilson
Well-known member
bioRxiv preprint
doi: https://doi.org/10.1101/2022.03.24.485633; this version posted March 28, 2022.
Chaitanya Kurhade1,#, Jing Zou1,#, Hongjie Xia1, Hui Cai2, Qi Yang2, Mark Cutler2, David Cooper2, Alexander Muik3, Kathrin U. Jansen2, Xuping Xie1,*, Kena A. Swanson2,*,Pei YongShi1,*,†
Abstract
The newly emerged Omicron SARS-CoV-2 has 3 distinct sublineages: BA.1, BA.2, and BA.3. BA.1 accounts for the initial surge and is being replaced by BA.2, whereas BA.3 is at a low prevalence at this time. Here we report the neutralization of BNT162b2-vaccinated sera (collected at 1 month after dose 3) against the three Omicron sublineages. To facilitate the neutralization testing, we engineered the complete BA.1, BA.2, or BA.3 spike into an mNeonGreen USA-WA1/2020 SRAS- CoV-2. All BNT162b2-vaccinated sera neutralized USA-WA1/2020, BA.1-, BA.2-, and BA.3-spike SARS-CoV-2s with titers of >20; the neutralization geometric mean titers (GMTs) against the four viruses were 1211, 336, 300, and 190, respectively. Thus, the BA.1-, BA.2-, and BA.3-spike SARS-CoV-2s were 3.6-, 4.0-, and 6.4-fold less effi- ciently neutralized than the USA-WA1/2020, respectively. Our data have implications in vaccine strategy and understanding the biology of Omicron sublineages.
https://www.biorxiv.org/content/10.1101/2022.03.24.485633v1.full.pdf
doi: https://doi.org/10.1101/2022.03.24.485633; this version posted March 28, 2022.
Chaitanya Kurhade1,#, Jing Zou1,#, Hongjie Xia1, Hui Cai2, Qi Yang2, Mark Cutler2, David Cooper2, Alexander Muik3, Kathrin U. Jansen2, Xuping Xie1,*, Kena A. Swanson2,*,Pei YongShi1,*,†
Abstract
The newly emerged Omicron SARS-CoV-2 has 3 distinct sublineages: BA.1, BA.2, and BA.3. BA.1 accounts for the initial surge and is being replaced by BA.2, whereas BA.3 is at a low prevalence at this time. Here we report the neutralization of BNT162b2-vaccinated sera (collected at 1 month after dose 3) against the three Omicron sublineages. To facilitate the neutralization testing, we engineered the complete BA.1, BA.2, or BA.3 spike into an mNeonGreen USA-WA1/2020 SRAS- CoV-2. All BNT162b2-vaccinated sera neutralized USA-WA1/2020, BA.1-, BA.2-, and BA.3-spike SARS-CoV-2s with titers of >20; the neutralization geometric mean titers (GMTs) against the four viruses were 1211, 336, 300, and 190, respectively. Thus, the BA.1-, BA.2-, and BA.3-spike SARS-CoV-2s were 3.6-, 4.0-, and 6.4-fold less effi- ciently neutralized than the USA-WA1/2020, respectively. Our data have implications in vaccine strategy and understanding the biology of Omicron sublineages.
https://www.biorxiv.org/content/10.1101/2022.03.24.485633v1.full.pdf
