tetano
Editor, Senior Moderator
Bioorg Chem
. 2025 Apr 18:160:108495.
doi: 10.1016/j.bioorg.2025.108495. Online ahead of print. A fluorometric and naked eye readable neuraminidase probe for influenza virus detection
Hao Chang[SUP] 1 [/SUP], Xin Zhao[SUP] 2 [/SUP], Zhenyou Jiang[SUP] 3 [/SUP], Yue Chen[SUP] 4 [/SUP], Zhiqi Lao[SUP] 5 [/SUP]
Affiliations
Rapid diagnostic methods are crucial for effective influenza therapy and the prevention of flu pandemics. Neuraminidase (NA), a key protein on the surface of influenza virions, plays a vital role in the replication of the influenza virus (IV). Herein, we present SANP, a multimode NA-activatable fluorescent probe designed for rapid influenza detection and live-cell imaging. This probe demonstrated a high sensitivity with a limit of detection (LOD) of 15.4 mU/mL for NA and 2[SUP]-2[/SUP] hemagglutination units (0.25 HAU) for influenza virus samples. SANP also exhibited a LOD of 2[SUP]0[/SUP] HAU (1 HAU) when detected influenza virus samples by the naked eye without the aid of equipment. Moreover, SANP facilitated real-time visualization of NA activity in influenza-infected cells through fluorescence imaging.
Keywords: Colorimetric detection; Fluorescent probe; Influenza virus; Neuraminidase; Sialic acid.
. 2025 Apr 18:160:108495.
doi: 10.1016/j.bioorg.2025.108495. Online ahead of print. A fluorometric and naked eye readable neuraminidase probe for influenza virus detection
Hao Chang[SUP] 1 [/SUP], Xin Zhao[SUP] 2 [/SUP], Zhenyou Jiang[SUP] 3 [/SUP], Yue Chen[SUP] 4 [/SUP], Zhiqi Lao[SUP] 5 [/SUP]
Affiliations
- PMID: 40280010
- DOI: 10.1016/j.bioorg.2025.108495
Rapid diagnostic methods are crucial for effective influenza therapy and the prevention of flu pandemics. Neuraminidase (NA), a key protein on the surface of influenza virions, plays a vital role in the replication of the influenza virus (IV). Herein, we present SANP, a multimode NA-activatable fluorescent probe designed for rapid influenza detection and live-cell imaging. This probe demonstrated a high sensitivity with a limit of detection (LOD) of 15.4 mU/mL for NA and 2[SUP]-2[/SUP] hemagglutination units (0.25 HAU) for influenza virus samples. SANP also exhibited a LOD of 2[SUP]0[/SUP] HAU (1 HAU) when detected influenza virus samples by the naked eye without the aid of equipment. Moreover, SANP facilitated real-time visualization of NA activity in influenza-infected cells through fluorescence imaging.
Keywords: Colorimetric detection; Fluorescent probe; Influenza virus; Neuraminidase; Sialic acid.