tetano
Editor, Senior Moderator
Biomed J
. 2020 Dec 29;S2319-4170(20)30235-3.
doi: 10.1016/j.bj.2020.12.007. Online ahead of print.
Comparison of the Roche cobas 6800 SARS-CoV-2 test and the Taiwan CDC protocol for the molecular diagnosis of COVID-19
Huey-Ling You[SUP] 1 [/SUP], Meng-Chih Lin[SUP] 2 [/SUP], Chen-Hsiang Lee[SUP] 3 [/SUP]
Affiliations
Abstract
The current coronavirus disease 2019 (COVID-19) pandemic has caused significant challenges throughout the world and a rapid, reliable diagnostic test is in high demand. Real-time reverse transcription polymerase chain reaction (RT-PCR) was one of the most quickly established methods of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection and is considered to be the gold standard. In this report, we share our experience of using two different testing platforms: the cobas 6800 SARS-CoV-2 test, an automated system that was recently granted Emergency Use Authorization by the FDA, and a laboratory-developed test based on the protocol from the Taiwan Centers for Disease Control (CDC). There was an overall 96.2% agreement between the two platforms. However, the positive agreement between the two platforms was only 80.0%. We found 3 instances of discordance between the two systems and this emphasized the need for timely diagnosis with a reliable testing platform.
Keywords: Coronavirus; Molecular diagnostics; RNA extraction; RT-PCR; Viral load.
. 2020 Dec 29;S2319-4170(20)30235-3.
doi: 10.1016/j.bj.2020.12.007. Online ahead of print.
Comparison of the Roche cobas 6800 SARS-CoV-2 test and the Taiwan CDC protocol for the molecular diagnosis of COVID-19
Huey-Ling You[SUP] 1 [/SUP], Meng-Chih Lin[SUP] 2 [/SUP], Chen-Hsiang Lee[SUP] 3 [/SUP]
Affiliations
- PMID: 33736952
- DOI: 10.1016/j.bj.2020.12.007
Abstract
The current coronavirus disease 2019 (COVID-19) pandemic has caused significant challenges throughout the world and a rapid, reliable diagnostic test is in high demand. Real-time reverse transcription polymerase chain reaction (RT-PCR) was one of the most quickly established methods of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection and is considered to be the gold standard. In this report, we share our experience of using two different testing platforms: the cobas 6800 SARS-CoV-2 test, an automated system that was recently granted Emergency Use Authorization by the FDA, and a laboratory-developed test based on the protocol from the Taiwan Centers for Disease Control (CDC). There was an overall 96.2% agreement between the two platforms. However, the positive agreement between the two platforms was only 80.0%. We found 3 instances of discordance between the two systems and this emphasized the need for timely diagnosis with a reliable testing platform.
Keywords: Coronavirus; Molecular diagnostics; RNA extraction; RT-PCR; Viral load.