tetano
Editor, Senior Moderator
Biol Cell
. 2021 Feb 18.
doi: 10.1111/boc.202000146. Online ahead of print.
Intracellular host cell membrane remodelling induced by SARS-CoV-2 infection in vitro
Lucio Ayres Caldas[SUP] 1 2 [/SUP], Fabiana Avila Carneiro[SUP] 1 [/SUP], Fabio Luis Monteiro[SUP] 3 [/SUP], Ingrid Augusto[SUP] 2 [/SUP], Luiza Mendon?a Higa[SUP] 3 [/SUP], Kildare Miranda[SUP] 2 4 [/SUP], Amilcar Tanuri[SUP] 3 [/SUP], Wanderley de Souza[SUP] 2 4 [/SUP]
Affiliations
Abstract
Background information: SARS-CoV-2 infection induces an alteration in the endomembrane system of the mammalian cells. In this study, we used transmission electron microscopy and electron tomography to investigate the main structural alterations in the cytoplasm of Vero cells infected with a SARS-CoV-2 isolate from S?o Paulo state (Brazil).
Results: Different membranous structures derived from the zippered endoplasmic reticulum were observed along with virus assembly through membrane budding. Also, we demonstrated the occurrence of annulate lamellae in the cytoplasm of infected cells and the presence of virus particles in the perinuclear space.
Conclusions and significance: This study contributes to a better understanding of the cell biology of SARS-CoV-2 and the mechanisms of the interaction of the virus with the host cell that promote morphological changes, recruitment of organelles, and cell components, in a context of a virus-induced membrane remodelling. This article is protected by copyright. All rights reserved.
Keywords: Coronavirus morphogenesis; Electron microscopy; SARS-CoV-2.
. 2021 Feb 18.
doi: 10.1111/boc.202000146. Online ahead of print.
Intracellular host cell membrane remodelling induced by SARS-CoV-2 infection in vitro
Lucio Ayres Caldas[SUP] 1 2 [/SUP], Fabiana Avila Carneiro[SUP] 1 [/SUP], Fabio Luis Monteiro[SUP] 3 [/SUP], Ingrid Augusto[SUP] 2 [/SUP], Luiza Mendon?a Higa[SUP] 3 [/SUP], Kildare Miranda[SUP] 2 4 [/SUP], Amilcar Tanuri[SUP] 3 [/SUP], Wanderley de Souza[SUP] 2 4 [/SUP]
Affiliations
- PMID: 33600624
- DOI: 10.1111/boc.202000146
Abstract
Background information: SARS-CoV-2 infection induces an alteration in the endomembrane system of the mammalian cells. In this study, we used transmission electron microscopy and electron tomography to investigate the main structural alterations in the cytoplasm of Vero cells infected with a SARS-CoV-2 isolate from S?o Paulo state (Brazil).
Results: Different membranous structures derived from the zippered endoplasmic reticulum were observed along with virus assembly through membrane budding. Also, we demonstrated the occurrence of annulate lamellae in the cytoplasm of infected cells and the presence of virus particles in the perinuclear space.
Conclusions and significance: This study contributes to a better understanding of the cell biology of SARS-CoV-2 and the mechanisms of the interaction of the virus with the host cell that promote morphological changes, recruitment of organelles, and cell components, in a context of a virus-induced membrane remodelling. This article is protected by copyright. All rights reserved.
Keywords: Coronavirus morphogenesis; Electron microscopy; SARS-CoV-2.