tetano
Editor, Senior Moderator
Bioessays
. 2020 Nov 17;e2000240.
doi: 10.1002/bies.202000240. Online ahead of print.
The genetic structure of SARS-CoV-2 does not rule out a laboratory origin: SARS-COV-2 chimeric structure and furin cleavage site might be the result of genetic manipulation
Rossana Segreto[SUP] 1 [/SUP], Yuri Deigin[SUP] 2 [/SUP]
Affiliations
Abstract
Severe acute respiratory syndrome-coronavirus (SARS-CoV)-2's origin is still controversial. Genomic analyses show SARS-CoV-2 likely to be chimeric, most of its sequence closest to bat CoV RaTG13, whereas its receptor binding domain (RBD) is almost identical to that of a pangolin CoV. Chimeric viruses can arise via natural recombination or human intervention. The furin cleavage site in the spike protein of SARS-CoV-2 confers to the virus the ability to cross species and tissue barriers, but was previously unseen in other SARS-like CoVs. Might genetic manipulations have been performed in order to evaluate pangolins as possible intermediate hosts for bat-derived CoVs that were originally unable to bind to human receptors? Both cleavage site and specific RBD could result from site-directed mutagenesis, a procedure that does not leave a trace. Considering the devastating impact of SARS-CoV-2 and importance of preventing future pandemics, researchers have a responsibility to carry out a thorough analysis of all possible SARS-CoV-2 origins.
Keywords: BtCov/4991; Gain-of-function studies; RaTG13; SARS-CoV-2; furin cleavage site; pangolin CoV; receptor binding domain.
. 2020 Nov 17;e2000240.
doi: 10.1002/bies.202000240. Online ahead of print.
The genetic structure of SARS-CoV-2 does not rule out a laboratory origin: SARS-COV-2 chimeric structure and furin cleavage site might be the result of genetic manipulation
Rossana Segreto[SUP] 1 [/SUP], Yuri Deigin[SUP] 2 [/SUP]
Affiliations
- PMID: 33200842
- DOI: 10.1002/bies.202000240
Abstract
Severe acute respiratory syndrome-coronavirus (SARS-CoV)-2's origin is still controversial. Genomic analyses show SARS-CoV-2 likely to be chimeric, most of its sequence closest to bat CoV RaTG13, whereas its receptor binding domain (RBD) is almost identical to that of a pangolin CoV. Chimeric viruses can arise via natural recombination or human intervention. The furin cleavage site in the spike protein of SARS-CoV-2 confers to the virus the ability to cross species and tissue barriers, but was previously unseen in other SARS-like CoVs. Might genetic manipulations have been performed in order to evaluate pangolins as possible intermediate hosts for bat-derived CoVs that were originally unable to bind to human receptors? Both cleavage site and specific RBD could result from site-directed mutagenesis, a procedure that does not leave a trace. Considering the devastating impact of SARS-CoV-2 and importance of preventing future pandemics, researchers have a responsibility to carry out a thorough analysis of all possible SARS-CoV-2 origins.
Keywords: BtCov/4991; Gain-of-function studies; RaTG13; SARS-CoV-2; furin cleavage site; pangolin CoV; receptor binding domain.
