tetano
Editor, Senior Moderator
Bioelectrochemistry
. 2021 Oct 15;143:107982.
doi: 10.1016/j.bioelechem.2021.107982. Online ahead of print.
Carbon nanotube field-effect transistor (CNT-FET)-based biosensor for rapid detection of SARS-CoV-2 (COVID-19) surface spike protein S1
Mazin A Zamzami[SUP] 1 [/SUP], Gulam Rabbani[SUP] 2 [/SUP], Abrar Ahmad[SUP] 3 [/SUP], Ahmad A Basalah[SUP] 4 [/SUP], Wesam H Al-Sabban[SUP] 5 [/SUP], Saeyoung Nate Ahn[SUP] 6 [/SUP], Hani Choudhry[SUP] 7 [/SUP]
Affiliations
Abstract
The large-scale diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is important for traceability and treatment during pandemic outbreaks. We developed a fast (2-3 min), easy-to-use, low-cost, and quantitative electrochemical biosensor based on carbon nanotube field-effect transistor (CNT-FET) that allows digital detection of the SARS-CoV-2 S1 in fortifited saliva samples for quick and accurate detection of SARS-CoV-2 S1 antigens. The biosensor was developed on a Si/SiO[SUB]2[/SUB] surface by CNT printing with the immobilization of a anti-SARS-CoV-2 S1. SARS-CoV-2 S1 antibody was immobilized on the CNT surface between the S-D channel area using a linker 1-pyrenebutanoic acid succinimidyl ester (PBASE) through non-covalent interaction. A commercial SARS-CoV-2 S1 antigen was used to characterize the electrical output of the CNT-FET biosensor. The SARS-CoV-2 S1 antigen in the 10 mM AA buffer pH 6.0 was effectively detected by the CNT-FET biosensor at concentrations from 0.1 fg/mL to 5.0 pg/mL. The limit of detection (LOD) of the developed CNT-FET biosensor was 4.12 fg/mL. The selectivity test was performed by using target SARS-CoV-2 S1 and non-target SARS-CoV-1 S1 and MERS-CoV S1 antigens in the 10 mM AA buffer pH 6.0. The biosensor showed high selectivity (no response to SARS-CoV-1 S1 or MERS-CoV S1 antigen) with SARS-CoV-2 S1 antigen detection in the 10 mM AA buffer pH 6.0. The biosensor is highly sensitive, saves time, and could be a helpful platform for rapid detection of SARS-CoV-2 S1 antigen from the patients saliva.
Keywords: Biosensor; Carbon nanotubes; Electrical immunosensor; Field-effect transistor; Severe acute respiratory syndrome coronavirus-2.
. 2021 Oct 15;143:107982.
doi: 10.1016/j.bioelechem.2021.107982. Online ahead of print.
Carbon nanotube field-effect transistor (CNT-FET)-based biosensor for rapid detection of SARS-CoV-2 (COVID-19) surface spike protein S1
Mazin A Zamzami[SUP] 1 [/SUP], Gulam Rabbani[SUP] 2 [/SUP], Abrar Ahmad[SUP] 3 [/SUP], Ahmad A Basalah[SUP] 4 [/SUP], Wesam H Al-Sabban[SUP] 5 [/SUP], Saeyoung Nate Ahn[SUP] 6 [/SUP], Hani Choudhry[SUP] 7 [/SUP]
Affiliations
- PMID: 34715586
- PMCID: PMC8518145
- DOI: 10.1016/j.bioelechem.2021.107982
Abstract
The large-scale diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is important for traceability and treatment during pandemic outbreaks. We developed a fast (2-3 min), easy-to-use, low-cost, and quantitative electrochemical biosensor based on carbon nanotube field-effect transistor (CNT-FET) that allows digital detection of the SARS-CoV-2 S1 in fortifited saliva samples for quick and accurate detection of SARS-CoV-2 S1 antigens. The biosensor was developed on a Si/SiO[SUB]2[/SUB] surface by CNT printing with the immobilization of a anti-SARS-CoV-2 S1. SARS-CoV-2 S1 antibody was immobilized on the CNT surface between the S-D channel area using a linker 1-pyrenebutanoic acid succinimidyl ester (PBASE) through non-covalent interaction. A commercial SARS-CoV-2 S1 antigen was used to characterize the electrical output of the CNT-FET biosensor. The SARS-CoV-2 S1 antigen in the 10 mM AA buffer pH 6.0 was effectively detected by the CNT-FET biosensor at concentrations from 0.1 fg/mL to 5.0 pg/mL. The limit of detection (LOD) of the developed CNT-FET biosensor was 4.12 fg/mL. The selectivity test was performed by using target SARS-CoV-2 S1 and non-target SARS-CoV-1 S1 and MERS-CoV S1 antigens in the 10 mM AA buffer pH 6.0. The biosensor showed high selectivity (no response to SARS-CoV-1 S1 or MERS-CoV S1 antigen) with SARS-CoV-2 S1 antigen detection in the 10 mM AA buffer pH 6.0. The biosensor is highly sensitive, saves time, and could be a helpful platform for rapid detection of SARS-CoV-2 S1 antigen from the patients saliva.
Keywords: Biosensor; Carbon nanotubes; Electrical immunosensor; Field-effect transistor; Severe acute respiratory syndrome coronavirus-2.