tetano
Editor, Senior Moderator
Biochem Biophys Res Commun
. 2021 Sep 15;579:141-145.
doi: 10.1016/j.bbrc.2021.09.024. Online ahead of print.
SARS-CoV-2 triggers DNA damage response in Vero E6 cells
Joshua Victor[SUP] 1 [/SUP], Jamie Deutsch[SUP] 1 [/SUP], Annalis Whitaker[SUP] 2 [/SUP], Erica N Lamkin[SUP] 1 [/SUP], Anthony March[SUP] 1 [/SUP], Pei Zhou[SUP] 3 [/SUP], Jason W Botten[SUP] 4 [/SUP], Nimrat Chatterjee[SUP] 5 [/SUP]
Affiliations
Abstract
The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus responsible for the current COVID-19 pandemic and has now infected more than 200 million people with more than 4 million deaths globally. Recent data suggest that symptoms and general malaise may continue long after the infection has ended in recovered patients, suggesting that SARS-CoV-2 infection has profound consequences in the host cells. Here we report that SARS-CoV-2 infection can trigger a DNA damage response (DDR) in African green monkey kidney cells (Vero E6). We observed a transcriptional upregulation of the Ataxia telangiectasia and Rad3 related protein (ATR) in infected cells. In addition, we observed enhanced phosphorylation of CHK1, a downstream effector of the ATR DNA damage response, as well as H2AX. Strikingly, SARS-CoV-2 infection lowered the expression of TRF2 shelterin-protein complex, and reduced telomere lengths in infected Vero E6 cells. Thus, our observations suggest SARS-CoV-2 may have pathological consequences to host cells beyond evoking an immunopathogenic immune response.
Keywords: DNA damage Response; Genome instability; SARS-CoV-2; Telomeres.
. 2021 Sep 15;579:141-145.
doi: 10.1016/j.bbrc.2021.09.024. Online ahead of print.
SARS-CoV-2 triggers DNA damage response in Vero E6 cells
Joshua Victor[SUP] 1 [/SUP], Jamie Deutsch[SUP] 1 [/SUP], Annalis Whitaker[SUP] 2 [/SUP], Erica N Lamkin[SUP] 1 [/SUP], Anthony March[SUP] 1 [/SUP], Pei Zhou[SUP] 3 [/SUP], Jason W Botten[SUP] 4 [/SUP], Nimrat Chatterjee[SUP] 5 [/SUP]
Affiliations
- PMID: 34600299
- DOI: 10.1016/j.bbrc.2021.09.024
Abstract
The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus responsible for the current COVID-19 pandemic and has now infected more than 200 million people with more than 4 million deaths globally. Recent data suggest that symptoms and general malaise may continue long after the infection has ended in recovered patients, suggesting that SARS-CoV-2 infection has profound consequences in the host cells. Here we report that SARS-CoV-2 infection can trigger a DNA damage response (DDR) in African green monkey kidney cells (Vero E6). We observed a transcriptional upregulation of the Ataxia telangiectasia and Rad3 related protein (ATR) in infected cells. In addition, we observed enhanced phosphorylation of CHK1, a downstream effector of the ATR DNA damage response, as well as H2AX. Strikingly, SARS-CoV-2 infection lowered the expression of TRF2 shelterin-protein complex, and reduced telomere lengths in infected Vero E6 cells. Thus, our observations suggest SARS-CoV-2 may have pathological consequences to host cells beyond evoking an immunopathogenic immune response.
Keywords: DNA damage Response; Genome instability; SARS-CoV-2; Telomeres.