Giuseppe
Emeritus
Fab MAbs Specific to HA of Influenza Virus with H5N1 Neutralizing Activity Selected from Immunized Chicken Phage Library. (Biochem Biophys Res Commun., abstract, edited)
[Source: US National Library of Medicine, (LINK). Edited.]
Biochem Biophys Res Commun. 2010 Apr 8. [Epub ahead of print]
Fab MAbs Specific to HA of Influenza Virus with H5N1 Neutralizing Activity Selected from Immunized Chicken Phage Library.
Pitaksajjakul P, Lekcharoensuk P, Uparagarin N, Iii CF, Ibrahim MS, Ikuta K, Ramasoota P. - Center of Excellence for Antibody Research (CEAR), and Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Hemagglutinin protein (HA) was considered to be the primary target for monoclonal antibody production. This protein not only plays an important role in viral infections, but can also be used to differentiate H5N1 virus from other influenza A viruses. Hence, for diagnostic and therapeutic applications, it is important to develop anti-HA monoclonal antibody (MAb) with high sensitivity, specificity, stability, and productivity. Nine unique Fab MAbs were generated from chimeric chicken/human Fab phage display library constructed from cDNA derived from chickens immunized with recombinant hemagglutinin protein constructed from H5N1 Avian Influenza virus (A/Vietnam/1203/04). The obtained Fab MAbs showed several characteristics for further optimization and development--3 clones were highly specific to only H5N1 virus. This finding can be applied to the development of H5N1 diagnostic testing. Another clone showed neutralization activity that inhibited H5N1 influenza virus infection in Madin-Darby canine kidney (MDCK) cells. In addition, one clone showed strong reactivity with several of the influenza A virus subtypes tested. The conversion of this clone to whole IgG is a promising study for a cross-neutralization activity test.
Copyright ? 2010. Published by Elsevier Inc.
PMID: 20382115 [PubMed - as supplied by publisher]
-
------
[Source: US National Library of Medicine, (LINK). Edited.]
Biochem Biophys Res Commun. 2010 Apr 8. [Epub ahead of print]
Fab MAbs Specific to HA of Influenza Virus with H5N1 Neutralizing Activity Selected from Immunized Chicken Phage Library.
Pitaksajjakul P, Lekcharoensuk P, Uparagarin N, Iii CF, Ibrahim MS, Ikuta K, Ramasoota P. - Center of Excellence for Antibody Research (CEAR), and Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Hemagglutinin protein (HA) was considered to be the primary target for monoclonal antibody production. This protein not only plays an important role in viral infections, but can also be used to differentiate H5N1 virus from other influenza A viruses. Hence, for diagnostic and therapeutic applications, it is important to develop anti-HA monoclonal antibody (MAb) with high sensitivity, specificity, stability, and productivity. Nine unique Fab MAbs were generated from chimeric chicken/human Fab phage display library constructed from cDNA derived from chickens immunized with recombinant hemagglutinin protein constructed from H5N1 Avian Influenza virus (A/Vietnam/1203/04). The obtained Fab MAbs showed several characteristics for further optimization and development--3 clones were highly specific to only H5N1 virus. This finding can be applied to the development of H5N1 diagnostic testing. Another clone showed neutralization activity that inhibited H5N1 influenza virus infection in Madin-Darby canine kidney (MDCK) cells. In addition, one clone showed strong reactivity with several of the influenza A virus subtypes tested. The conversion of this clone to whole IgG is a promising study for a cross-neutralization activity test.
Copyright ? 2010. Published by Elsevier Inc.
PMID: 20382115 [PubMed - as supplied by publisher]
-
------