Giuseppe
Emeritus
Development of tetraphenylethylene-based fluorescent oligosaccharide probes for detection of influenza virus. (Biochem Biophys Res Commun., abstract, edited)
8. Biochem Biophys Res Commun. 2010 Feb 24. [Epub ahead of print]
Development of tetraphenylethylene-based fluorescent oligosaccharide probes for detection of influenza virus.
Kato T, Kawaguchi A, Nagata K, Hatanaka K. - Japan Chemical Innovation Institute, 1-3-5 Jimbocho, Chiyoda-ku, Tokyo 101-0051, Japan; Frontier Biochemical & Medical Research Laboratories, Kaneka Corporation, 1-8, Miyamae-machi, Takasago-cho, Takasago, Hyogo 676-8688, Japan.
Tetraphenylethylene (TPE) derivatives have strong fluorescece in aggregated state. We designed and synthesized a tetraphenylethylene derivative bearing alkyne groups which were used for combination by click chemistry. The new TPE compound bearing alkyne groups was used to synthesize fluorescensce oligosaccharide probes which have lactosyl and 6'-sialyllactosyl moieties as ligands. We found that the TPE compounds bearing lactosyl and 6'-sialyllactosyl moieties were useful for detection of RCA120 and SSA lectins, respectively. Moreover, we have shown that TPE-based fluorescent oligosaccharide probe bearing 6'-sialyllactose moiety can be utilized as a "turn-on" fluorescent sensor for detection of influenza virus.
Copyright ? 2010. Published by Elsevier Inc.
PMID: 20188703 [PubMed - as supplied by publisher]
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8. Biochem Biophys Res Commun. 2010 Feb 24. [Epub ahead of print]
Development of tetraphenylethylene-based fluorescent oligosaccharide probes for detection of influenza virus.
Kato T, Kawaguchi A, Nagata K, Hatanaka K. - Japan Chemical Innovation Institute, 1-3-5 Jimbocho, Chiyoda-ku, Tokyo 101-0051, Japan; Frontier Biochemical & Medical Research Laboratories, Kaneka Corporation, 1-8, Miyamae-machi, Takasago-cho, Takasago, Hyogo 676-8688, Japan.
Tetraphenylethylene (TPE) derivatives have strong fluorescece in aggregated state. We designed and synthesized a tetraphenylethylene derivative bearing alkyne groups which were used for combination by click chemistry. The new TPE compound bearing alkyne groups was used to synthesize fluorescensce oligosaccharide probes which have lactosyl and 6'-sialyllactosyl moieties as ligands. We found that the TPE compounds bearing lactosyl and 6'-sialyllactosyl moieties were useful for detection of RCA120 and SSA lectins, respectively. Moreover, we have shown that TPE-based fluorescent oligosaccharide probe bearing 6'-sialyllactose moiety can be utilized as a "turn-on" fluorescent sensor for detection of influenza virus.
Copyright ? 2010. Published by Elsevier Inc.
PMID: 20188703 [PubMed - as supplied by publisher]
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