tetano
Editor, Senior Moderator
Biochem Biophys Rep
. 2022 Dec;32:101379.
doi: 10.1016/j.bbrep.2022.101379. Epub 2022 Nov 7.
Comparison of the inactivation capacity of various UV wavelengths on SARS-CoV-2
Ryosuke Matsuura[SUP] 1 2 [/SUP], Chieh-Wen Lo[SUP] 1 2 3 [/SUP], Takayo Ogawa[SUP] 4 [/SUP], Masaru Nakagawa[SUP] 2 [/SUP], Masami Takei[SUP] 2 [/SUP], Yasunobu Matsumoto[SUP] 1 3 [/SUP], Satoshi Wada[SUP] 2 4 [/SUP], Yoko Aida[SUP] 1 2 3 [/SUP]
Affiliations
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused a worldwide pandemic. Ultraviolet (UV) is regarded as a very powerful tool against SARS-CoV-2. However, the inactivating effects of different UV wavelengths on SARS-CoV-2 under the same conditions have hardly been compared. Here, we showed that SARS-CoV-2 cultured in Dulbecco's modified Eagle's medium and 2% fetal bovine serum was efficiently inactivated by irradiation with 222, 254, and 265 wavelengths UV, but not at 308 nm. In addition, it was revealed that UV absorption by DMEM-2% FBS is very efficient at 222 nm. Our results present potentially important information for selecting the optimum UV wavelength according to the application.
Keywords: COVID-19; Inactivation; SARS-CoV-2; UV.
. 2022 Dec;32:101379.
doi: 10.1016/j.bbrep.2022.101379. Epub 2022 Nov 7.
Comparison of the inactivation capacity of various UV wavelengths on SARS-CoV-2
Ryosuke Matsuura[SUP] 1 2 [/SUP], Chieh-Wen Lo[SUP] 1 2 3 [/SUP], Takayo Ogawa[SUP] 4 [/SUP], Masaru Nakagawa[SUP] 2 [/SUP], Masami Takei[SUP] 2 [/SUP], Yasunobu Matsumoto[SUP] 1 3 [/SUP], Satoshi Wada[SUP] 2 4 [/SUP], Yoko Aida[SUP] 1 2 3 [/SUP]
Affiliations
- PMID: 36373011
- PMCID: PMC9638801
- DOI: 10.1016/j.bbrep.2022.101379
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused a worldwide pandemic. Ultraviolet (UV) is regarded as a very powerful tool against SARS-CoV-2. However, the inactivating effects of different UV wavelengths on SARS-CoV-2 under the same conditions have hardly been compared. Here, we showed that SARS-CoV-2 cultured in Dulbecco's modified Eagle's medium and 2% fetal bovine serum was efficiently inactivated by irradiation with 222, 254, and 265 wavelengths UV, but not at 308 nm. In addition, it was revealed that UV absorption by DMEM-2% FBS is very efficient at 222 nm. Our results present potentially important information for selecting the optimum UV wavelength according to the application.
Keywords: COVID-19; Inactivation; SARS-CoV-2; UV.