tetano
Editor, Senior Moderator
Molecules. 2013 Dec 13;18(12):15573-86. doi: 10.3390/molecules181215573.
Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots.
Krejcova L, Nejdl L, Hynek D, Krizkova S, Kopel P, Adam V, Kizek R.
Author information
Abstract
In this study we describe a beads-based assay for rapid, sensitive and specific isolation and detection of influenza vaccine hemagglutinin (HA). Amplification of the hemagglutinin signal resulted from binding of an electrochemical label as quantum dots (QDs). For detection of the metal and protein part of the resulting HA-CdTe complex, two differential pulse voltammetric methods were used. The procedure includes automated robotic isolation and electrochemical analysis of the isolated product. The isolation procedure was based on the binding of paramagnetic particles (MPs) with glycan (Gly), where glycan was used as the specific receptor for linkage of the QD-labeled hemagglutinin.
PMID:
24352014
[PubMed - in process]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/24352014
Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots.
Krejcova L, Nejdl L, Hynek D, Krizkova S, Kopel P, Adam V, Kizek R.
Author information
Abstract
In this study we describe a beads-based assay for rapid, sensitive and specific isolation and detection of influenza vaccine hemagglutinin (HA). Amplification of the hemagglutinin signal resulted from binding of an electrochemical label as quantum dots (QDs). For detection of the metal and protein part of the resulting HA-CdTe complex, two differential pulse voltammetric methods were used. The procedure includes automated robotic isolation and electrochemical analysis of the isolated product. The isolation procedure was based on the binding of paramagnetic particles (MPs) with glycan (Gly), where glycan was used as the specific receptor for linkage of the QD-labeled hemagglutinin.
PMID:
24352014
[PubMed - in process]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/24352014