Mary Wilson
Well-known member
Published: February 21, 2023
DOI: https://doi.org/10.1016/j.cell.2023.01.011
Marion De ́ josez,1,2,3 Arturo Marin,4,5,21 Graham M. Hughes,6,21 Ariadna E. Morales,8,9,21 Carlos Godoy-Parejo,1,2Jonathan L. Gray,1,2 Yiren Qin,1,2 Arun A. Singh,1,2 Hui Xu,1,2 Javier Juste,10,11 Carlos Iba ́ n ̃ ez,10 Kris M. White,4,5Romel Rosales,4,5 Nancy J. Francoeur,12 Robert P. Sebra,13,14 Dominic Alcock,6 Thomas L. Volkert,3
Se ́ bastien J. Puechmaille,15 Andrzej Pastusiak,16 Simon D.W. Frost,16,17 Michael Hiller,7,8,9 Richard A. Young,18Emma C. Teeling,6,* Adolfo Garcı ́a-Sastre,4,5,19,20,* and Thomas P. Zwaka1,2,3,22,*
SUMMARY
Bats are distinctive among mammals due to their ability to fly, use laryngeal echolocation, and tolerate vi- ruses. However, there are currently no reliable cellular models for studying bat biology or their response to viral infections. Here, we created induced pluripotent stem cells (iPSCs) from two species of bats: the wild greater horseshoe bat (Rhinolophus ferrumequinum) and the greater mouse-eared bat (Myotis myotis). The iPSCs from both bat species showed similar characteristics and had a gene expression profile resem- bling that of cells attacked by viruses. They also had a high number of endogenous viral sequences, partic- ularly retroviruses. These results suggest that bats have evolved mechanisms to tolerate a large load of viral sequences and may have a more intertwined relationship with viruses than previously thought. Further study of bat iPSCs and their differentiated progeny will provide insights into bat biology, virus host relationships, and the molecular basis of bats’ special traits.
https://www.cell.com/action/showPdf?pii=S0092-8674(23)00041-7
DOI: https://doi.org/10.1016/j.cell.2023.01.011
Marion De ́ josez,1,2,3 Arturo Marin,4,5,21 Graham M. Hughes,6,21 Ariadna E. Morales,8,9,21 Carlos Godoy-Parejo,1,2Jonathan L. Gray,1,2 Yiren Qin,1,2 Arun A. Singh,1,2 Hui Xu,1,2 Javier Juste,10,11 Carlos Iba ́ n ̃ ez,10 Kris M. White,4,5Romel Rosales,4,5 Nancy J. Francoeur,12 Robert P. Sebra,13,14 Dominic Alcock,6 Thomas L. Volkert,3
Se ́ bastien J. Puechmaille,15 Andrzej Pastusiak,16 Simon D.W. Frost,16,17 Michael Hiller,7,8,9 Richard A. Young,18Emma C. Teeling,6,* Adolfo Garcı ́a-Sastre,4,5,19,20,* and Thomas P. Zwaka1,2,3,22,*
SUMMARY
Bats are distinctive among mammals due to their ability to fly, use laryngeal echolocation, and tolerate vi- ruses. However, there are currently no reliable cellular models for studying bat biology or their response to viral infections. Here, we created induced pluripotent stem cells (iPSCs) from two species of bats: the wild greater horseshoe bat (Rhinolophus ferrumequinum) and the greater mouse-eared bat (Myotis myotis). The iPSCs from both bat species showed similar characteristics and had a gene expression profile resem- bling that of cells attacked by viruses. They also had a high number of endogenous viral sequences, partic- ularly retroviruses. These results suggest that bats have evolved mechanisms to tolerate a large load of viral sequences and may have a more intertwined relationship with viruses than previously thought. Further study of bat iPSCs and their differentiated progeny will provide insights into bat biology, virus host relationships, and the molecular basis of bats’ special traits.
https://www.cell.com/action/showPdf?pii=S0092-8674(23)00041-7