tetano
Editor, Senior Moderator
Avian Pathol
. 2024 Feb 12:1-42.
doi: 10.1080/03079457.2024.2317430. Online ahead of print. Genome sequences of hemagglutinin cleavage site predict the pathogenicity phenotype of avian influenza virus: statistically validated data for facilitating rapid declarations and reducing reliance on in vivo testing
Dong-Hun Lee[SUP] 1 [/SUP], Mia K Torchetti[SUP] 2 [/SUP], Mary Lea Killian[SUP] 2 [/SUP], Ian Brown[SUP] 3 [/SUP], David E Swayne[SUP] 4 [/SUP]
Affiliations
AbstractBased on the pathogenicity in chickens, most H1-H16 avian influenza (AI) viruses cause mild diseases, whereas some of the H5 and H7 AI viruses cause systemic disease. The number of basic amino acids in the hemagglutinin (HA) cleavage site of AI viruses plays a critical role in pathogenicity. As we gain a greater understanding of the molecular mechanisms of pathogenicity, genome sequencing of the HA0 cleavage site has assumed a greater role in assessment of the potential pathogenicity of H5 and H7 viruses. We validated the use of HA cleavage site motif analysis by comparing molecular pathotyping data against experimental in vivo (IVPI and lethality) data for determination of both low pathogenicity and high pathogenicity AI virus declaration with the goal of expediting pathotype confirmation and further reducing the reliance on in vivo testing. Our data provide statistical support to the continued use of molecular determination of pathotype for AI viruses based on the HA cleavage site sequence in absence of an in vivo study determination. This approach not only expedites the declaration process of HPAIV but also reduces the need for experimental in vivo testing of H5 and H7 viruses.
Keywords: avian influenza virus; cleavage site; hemagglutinin; highly pathogenic avian influenza virus; pathogenicity.
. 2024 Feb 12:1-42.
doi: 10.1080/03079457.2024.2317430. Online ahead of print. Genome sequences of hemagglutinin cleavage site predict the pathogenicity phenotype of avian influenza virus: statistically validated data for facilitating rapid declarations and reducing reliance on in vivo testing
Dong-Hun Lee[SUP] 1 [/SUP], Mia K Torchetti[SUP] 2 [/SUP], Mary Lea Killian[SUP] 2 [/SUP], Ian Brown[SUP] 3 [/SUP], David E Swayne[SUP] 4 [/SUP]
Affiliations
- PMID: 38345041
- DOI: 10.1080/03079457.2024.2317430
AbstractBased on the pathogenicity in chickens, most H1-H16 avian influenza (AI) viruses cause mild diseases, whereas some of the H5 and H7 AI viruses cause systemic disease. The number of basic amino acids in the hemagglutinin (HA) cleavage site of AI viruses plays a critical role in pathogenicity. As we gain a greater understanding of the molecular mechanisms of pathogenicity, genome sequencing of the HA0 cleavage site has assumed a greater role in assessment of the potential pathogenicity of H5 and H7 viruses. We validated the use of HA cleavage site motif analysis by comparing molecular pathotyping data against experimental in vivo (IVPI and lethality) data for determination of both low pathogenicity and high pathogenicity AI virus declaration with the goal of expediting pathotype confirmation and further reducing the reliance on in vivo testing. Our data provide statistical support to the continued use of molecular determination of pathotype for AI viruses based on the HA cleavage site sequence in absence of an in vivo study determination. This approach not only expedites the declaration process of HPAIV but also reduces the need for experimental in vivo testing of H5 and H7 viruses.
Keywords: avian influenza virus; cleavage site; hemagglutinin; highly pathogenic avian influenza virus; pathogenicity.