tetano
Editor, Senior Moderator
Avian Pathol
. 2023 Oct 27:1-37.
doi: 10.1080/03079457.2023.2276849. Online ahead of print. A real-time colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the rapid detection of highly pathogenic H5 clade 2.3.4.4b avian influenza viruses
Fabien Filaire[SUP] 1 2 [/SUP], Aurélie Sécula[SUP] 1 [/SUP], Laetitia Lebre[SUP] 1 [/SUP], Guillaume Croville[SUP] 1 [/SUP], Jean-Luc Guerin[SUP] 1 [/SUP]
Affiliations
Highly pathogenic avian influenza viruses (HPAIV) are a major threat to the global poultry industry and public health due to their zoonotic potential. Since 2016, Europe and France have faced major epizootics caused by clade 2.3.4.4b H5 HPAIV. To reduce sample-to-result times, point-of-care testing is urgently needed to help prevent further outbreaks and the propagation of the virus. This study presents the design of a novel real-time colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of clade 2.3.4.4b H5 HPAIV. A clinical validation of this RT-LAMP assay was performed on 198 pools of clinical swabs sampled in 52 poultry flocks during the H5 HPAI 2020-2022 epizootics in France. This RT-LAMP assay allowed the specific detection of HPAIV H5Nx clade 2.3.4.4b within 30 minutes with a sensitivity of 86.11%. This rapid, easy-to-perform, inexpensive, molecular detection assay could be included in the HPAIV surveillance toolbox.
Keywords: RT-LAMP; clade 2.3.4.4b molecular detection; clinical diagnostic; field detection; highly pathogenic avian influenza virus.
. 2023 Oct 27:1-37.
doi: 10.1080/03079457.2023.2276849. Online ahead of print. A real-time colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the rapid detection of highly pathogenic H5 clade 2.3.4.4b avian influenza viruses
Fabien Filaire[SUP] 1 2 [/SUP], Aurélie Sécula[SUP] 1 [/SUP], Laetitia Lebre[SUP] 1 [/SUP], Guillaume Croville[SUP] 1 [/SUP], Jean-Luc Guerin[SUP] 1 [/SUP]
Affiliations
- PMID: 37885409
- DOI: 10.1080/03079457.2023.2276849
Highly pathogenic avian influenza viruses (HPAIV) are a major threat to the global poultry industry and public health due to their zoonotic potential. Since 2016, Europe and France have faced major epizootics caused by clade 2.3.4.4b H5 HPAIV. To reduce sample-to-result times, point-of-care testing is urgently needed to help prevent further outbreaks and the propagation of the virus. This study presents the design of a novel real-time colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of clade 2.3.4.4b H5 HPAIV. A clinical validation of this RT-LAMP assay was performed on 198 pools of clinical swabs sampled in 52 poultry flocks during the H5 HPAI 2020-2022 epizootics in France. This RT-LAMP assay allowed the specific detection of HPAIV H5Nx clade 2.3.4.4b within 30 minutes with a sensitivity of 86.11%. This rapid, easy-to-perform, inexpensive, molecular detection assay could be included in the HPAIV surveillance toolbox.
Keywords: RT-LAMP; clade 2.3.4.4b molecular detection; clinical diagnostic; field detection; highly pathogenic avian influenza virus.