tetano
Editor, Senior Moderator
Methods Mol Biol. 2014;1161:93-104. doi: 10.1007/978-1-4939-0758-8_9.
Avian influenza virus RNA extraction.
Spackman E1, Lee SA.
Author information
Abstract
The efficient extraction and purification of viral RNA is critical for down-stream molecular applications whether it is the sensitive and specific detection of virus in clinical samples, virus gene cloning and expression, or quantification of avian influenza (AI) virus by molecular methods from experimentally infected birds. Samples can generally be divided into two types; enriched (e.g. virus stocks) and clinical. Clinical type samples, which may be tissues or swab material, are the most difficult to process due to the complex sample composition and possibly low virus titers. In this chapter two well established procedures for the isolation of AI virus RNA from common clinical specimen types and enriched virus stocks for further molecular applications will be presented.
PMID:
24899423
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24899423
Avian influenza virus RNA extraction.
Spackman E1, Lee SA.
Author information
Abstract
The efficient extraction and purification of viral RNA is critical for down-stream molecular applications whether it is the sensitive and specific detection of virus in clinical samples, virus gene cloning and expression, or quantification of avian influenza (AI) virus by molecular methods from experimentally infected birds. Samples can generally be divided into two types; enriched (e.g. virus stocks) and clinical. Clinical type samples, which may be tissues or swab material, are the most difficult to process due to the complex sample composition and possibly low virus titers. In this chapter two well established procedures for the isolation of AI virus RNA from common clinical specimen types and enriched virus stocks for further molecular applications will be presented.
PMID:
24899423
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24899423