Mary Wilson
Well-known member
16 January 2023
DOI: doi/10.1128/mbio.03399-22
Authors: Olivia C. Swann, Amalie B. Rasmussen, Thomas P. Peacock, Carol M. Sheppard , Wendy S.Barclay
ABSTRACT
Host restriction limits the emergence of novel pandemic strains from the influenza A virus avian reservoir. For efficient replication in mammalian cells, the avian influenza RNA-dependent RNA polymerase must adapt to use human orthologues of the host factor ANP32, which lack a 33-amino-acid insertion relative to avian ANP32A. Here, we find that influenza polymerase requires ANP32 proteins to support both steps of genome replication: cRNA and vRNA synthesis. However, avian strains are only restricted in vRNA synthesis in human cells. Therefore, avian influenza polymerase can use human ANP32 orthologues to support cRNA synthesis, without acquiring mammalian adaptations. This implies a fundamental difference in the mechanism by which ANP32 proteins support cRNA versus vRNA synthesis.
https://journals.asm.org/doi/10.1128/mbio.03399-22
DOI: doi/10.1128/mbio.03399-22
Authors: Olivia C. Swann, Amalie B. Rasmussen, Thomas P. Peacock, Carol M. Sheppard , Wendy S.Barclay
ABSTRACT
Host restriction limits the emergence of novel pandemic strains from the influenza A virus avian reservoir. For efficient replication in mammalian cells, the avian influenza RNA-dependent RNA polymerase must adapt to use human orthologues of the host factor ANP32, which lack a 33-amino-acid insertion relative to avian ANP32A. Here, we find that influenza polymerase requires ANP32 proteins to support both steps of genome replication: cRNA and vRNA synthesis. However, avian strains are only restricted in vRNA synthesis in human cells. Therefore, avian influenza polymerase can use human ANP32 orthologues to support cRNA synthesis, without acquiring mammalian adaptations. This implies a fundamental difference in the mechanism by which ANP32 proteins support cRNA versus vRNA synthesis.
https://journals.asm.org/doi/10.1128/mbio.03399-22