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Associations between Viral and Bacterial Potential Pathogens in the Nasopharynx of Children with and Without Respiratory Symptoms

tetano

Editor, Senior Moderator
Pediatr Infect Dis J. 2015 Aug 10. [Epub ahead of print]
[h=1]Associations between Viral and Bacterial Potential Pathogens in the Nasopharynx of Children with and Without Respiratory Symptoms.[/h] Skevaki CL[SUP]1[/SUP], Tsialta P, Trochoutsou AI, Logotheti I, Makrinioti H, Taka S, Lebessi E, Paraskakis I, Papadopoulos NG, Tsolia MN.
[h=3]Author information[/h]

[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Nasopharyngeal bacterial colonization is necessary for subsequent respiratory and/or invasive infection. Our study aimed atcomparing nasopharyngeal bacterial colonization rates between children with and without symptoms of an acute viral respiratory tract infection (ARTI), and examining associations between identified microorganisms.
[h=4]METHODS:[/h] Children 3 months-6 years old with and without an ARTI were recruitedand a questionnaire was filled. Nasopharyngeal samples were examined for Streptococcus pneumoniae(SP), Haemophilusinfluenzae(HI), Moraxella catarrhalis (MC), Staphylococcus aureus (SA) and Streptococcus pyogenes(SPyo) by culture.Viruses were detected with polymerase chain reaction (PCR).
[h=4]RESULTS:[/h] Median age of the 386recruited children was 23.4 months, and 127 had no respiratory symptoms. More asymptomatic subjects were found negative for all bacteria tested (p<0.01). SP (p<0.01), MC (p=0.001) and mixed bacterial colonization patterns were more frequent among symptomatic children (p<0.05). Colonization of symptomatic, virus positive children with MC was higher than in asymptomatic and/or virus negative children (p=0.005).The highest HI and MC colonization rates were recorded in association with influenza virus. A strongly negative association between SP and SA, a higher rate of HI detection among SP colonized children, and an increased likelihood of MC detection in the presence of HI were observed. HI colonization was more likely in the presence of RSV and MC colonization was associated with RV detection.
[h=4]CONCLUSIONS:[/h] Viruses are associated with different nasopharyngeal bacterial colonization patterns.Observedpathogens'associations may play a role in disease, and continuous surveillance is required to follow possible effects of interventions such as vaccines.


PMID: 26262821 [PubMed - as supplied by publisher]
 
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