tetano
Editor, Senior Moderator
New Microbiol. 2011 Oct;34(4):391-7. Epub 2011 Oct 31.
Assembly and characterization of pandemic influenza A H1N1 genome in nasopharyngeal swabs using high-throughput pyrosequencing.
Bartolini B, Chillemi G, Abbate I, Bruselles A, Rozera G, Castrignan? T, Paoletti D, Picardi E, Desideri A, Pesole G, Capobianchi MR.
Source
National Institute for Infectious Diseases (INMI) L. Spallanzani, Rome, Italy.
Abstract
De novo high-throughput pyrosequencing was used to detect and characterize 2009 pandemic influenza A (H1N1) virus directly in nasopharyngeal swabs in the context of the microbial community. Data were generated with a prior sequenceindependent amplification by 454 pyrosequencing on GS-FLX platform (Roche). Influenza A assembled reads allowed near full-length genome reconstruction with the simultaneous analysis of site-specific heterogeneity. The molecular approach applied proved to be a powerful tool to characterize the new pandemic H1N1 influenza virus in clinical samples. This approach could be of great value in identifying possibly new reassortants that may occur in the near future.
PMID:
22143812
[PubMed - in process]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/22143812
Assembly and characterization of pandemic influenza A H1N1 genome in nasopharyngeal swabs using high-throughput pyrosequencing.
Bartolini B, Chillemi G, Abbate I, Bruselles A, Rozera G, Castrignan? T, Paoletti D, Picardi E, Desideri A, Pesole G, Capobianchi MR.
Source
National Institute for Infectious Diseases (INMI) L. Spallanzani, Rome, Italy.
Abstract
De novo high-throughput pyrosequencing was used to detect and characterize 2009 pandemic influenza A (H1N1) virus directly in nasopharyngeal swabs in the context of the microbial community. Data were generated with a prior sequenceindependent amplification by 454 pyrosequencing on GS-FLX platform (Roche). Influenza A assembled reads allowed near full-length genome reconstruction with the simultaneous analysis of site-specific heterogeneity. The molecular approach applied proved to be a powerful tool to characterize the new pandemic H1N1 influenza virus in clinical samples. This approach could be of great value in identifying possibly new reassortants that may occur in the near future.
PMID:
22143812
[PubMed - in process]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/22143812