tetano
Editor, Senior Moderator
Vaccine. 2014 Jun 18. pii: S0264-410X(14)00829-9. doi: 10.1016/j.vaccine.2014.06.040. [Epub ahead of print]
AS04-adjuvanted virus-like particles containing multiple M2 extracellular domains of influenza virus confer improved protection.
Lee YN1, Kim MC2, Lee YT1, Hwang HS1, Cho MK1, Lee JS1, Ko EJ1, Kwon YM1, Kang SM3.
Author information
Abstract
The ectodomain of matrix protein 2 (M2e) of influenza virus is suggested to be a rational target for a universal influenza A vaccine. However, there are some concerns that M2e vaccines might not be highly effective in the general population with diverse genetic backgrounds. Here we examined the immunogenicity and protective efficacy of the baculovirus-derived virus-like particles containing multiple M2e (M2eVLP) with AS04 adjuvant in a C57BL/6 mouse strain (H-2b). M2eVLP vaccine induced significant levels of M2e-specific IgG in C57BL/6 mice after vaccination. Furthermore, M2eVLP adjuvanted with AS04 was more effective than M2eVLP alone in conferring protection as well as in inducing recall humoral and T cell responses specific for M2e after lethal influenza virus challenge. A mechanistic study provides evidence that activation of dendritic cells by the toll-like receptor 4 agonist MPL in the AS04 adjuvant was associated with interferon-γ producing CD4 T cell responses. Our results suggest that AS04 adjuvanted M2eVLP vaccines have the potential to improve cross-protection.
Copyright ? 2014. Published by Elsevier Ltd.
KEYWORDS:
Adjuvants; C57BL/6; Influenza virus; M2e VLPs
PMID:
24951867
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24951867
AS04-adjuvanted virus-like particles containing multiple M2 extracellular domains of influenza virus confer improved protection.
Lee YN1, Kim MC2, Lee YT1, Hwang HS1, Cho MK1, Lee JS1, Ko EJ1, Kwon YM1, Kang SM3.
Author information
Abstract
The ectodomain of matrix protein 2 (M2e) of influenza virus is suggested to be a rational target for a universal influenza A vaccine. However, there are some concerns that M2e vaccines might not be highly effective in the general population with diverse genetic backgrounds. Here we examined the immunogenicity and protective efficacy of the baculovirus-derived virus-like particles containing multiple M2e (M2eVLP) with AS04 adjuvant in a C57BL/6 mouse strain (H-2b). M2eVLP vaccine induced significant levels of M2e-specific IgG in C57BL/6 mice after vaccination. Furthermore, M2eVLP adjuvanted with AS04 was more effective than M2eVLP alone in conferring protection as well as in inducing recall humoral and T cell responses specific for M2e after lethal influenza virus challenge. A mechanistic study provides evidence that activation of dendritic cells by the toll-like receptor 4 agonist MPL in the AS04 adjuvant was associated with interferon-γ producing CD4 T cell responses. Our results suggest that AS04 adjuvanted M2eVLP vaccines have the potential to improve cross-protection.
Copyright ? 2014. Published by Elsevier Ltd.
KEYWORDS:
Adjuvants; C57BL/6; Influenza virus; M2e VLPs
PMID:
24951867
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24951867