Giuseppe
Emeritus
Arch Virol. 2009 Jun 13. [Epub ahead of print]
The mouse Pol I terminator is more efficient than the hepatitis delta virus ribozyme in generating influenza-virus-like RNAs with precise 3' ends in a plasmid-only-based virus rescue system.
Feng L, Li F, Zheng X, Pan W, Zhou K, Liu Y, He H, Chen L. - Center for Vaccines and Biotherapeutics, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou International Business Incubator A3, Guangzhou Science City, 510663, Guangzhou, China.
Reverse genetics systems for generating recombinant influenza viruses are based on two different mechanisms for obtaining the 3' end of the viral RNA: one uses the self-cleaving hepatitis delta virus ribozyme (HDVR), and the other uses the murine RNA polymerase I (Pol I) terminator.
In this study, we employed EGFP and Renilla luciferase reporter constructs to compare the efficiency of both methods.
Our results indicate that the murine Pol I terminator was more efficient than the HDVR, which will be helpful in choosing an influenza virus rescue system, as well as in establishing other RNA virus rescue systems.
PMID: 19526191 [PubMed - as supplied by publisher]
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The mouse Pol I terminator is more efficient than the hepatitis delta virus ribozyme in generating influenza-virus-like RNAs with precise 3' ends in a plasmid-only-based virus rescue system.
Feng L, Li F, Zheng X, Pan W, Zhou K, Liu Y, He H, Chen L. - Center for Vaccines and Biotherapeutics, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou International Business Incubator A3, Guangzhou Science City, 510663, Guangzhou, China.
Reverse genetics systems for generating recombinant influenza viruses are based on two different mechanisms for obtaining the 3' end of the viral RNA: one uses the self-cleaving hepatitis delta virus ribozyme (HDVR), and the other uses the murine RNA polymerase I (Pol I) terminator.
In this study, we employed EGFP and Renilla luciferase reporter constructs to compare the efficiency of both methods.
Our results indicate that the murine Pol I terminator was more efficient than the HDVR, which will be helpful in choosing an influenza virus rescue system, as well as in establishing other RNA virus rescue systems.
PMID: 19526191 [PubMed - as supplied by publisher]
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