tetano
Editor, Senior Moderator
Arch Virol
. 2023 Jan 13;168(2):63.
doi: 10.1007/s00705-022-05696-0.
Influenza A virus strain PR/8/34, but neither HAM/2009 nor WSN/33, is transiently inhibited by the PB2-targeting drug paliperidone
Georgios-Dimitrios Panagiotidis[SUP] 1 [/SUP], Christin Müller[SUP] 2 3 [/SUP], Marco Binder[SUP] 4 [/SUP], Friedemann Weber[SUP] 5 6 [/SUP]
Affiliations
Abstract
Influenza A virus (FLUAV) is a significant human pathogen. In silico structural analysis (PMID 28628827) has suggested that the FDA-approved drug paliperidone interferes with the binding of the FLUAV polymerase subunit PB2 to the nucleoprotein NP. We found that paliperidone inhibits FLUAV A/PR/8/34 early after infection of canine MDCK II, human A549, and human primary bronchial cells, but not at late time points. No effect was detectable against the strains A/Hamburg/05/2009 and A/WSN/33. Moreover, paliperidone indeed disturbed the interaction between the PB2 and the NP of A/PR/8/34 and reduced early viral RNA and protein synthesis by approximately 50%. Thus, paliperidone has measurable but transient and virus-strain-restricted effects on FLUAV.
. 2023 Jan 13;168(2):63.
doi: 10.1007/s00705-022-05696-0.
Influenza A virus strain PR/8/34, but neither HAM/2009 nor WSN/33, is transiently inhibited by the PB2-targeting drug paliperidone
Georgios-Dimitrios Panagiotidis[SUP] 1 [/SUP], Christin Müller[SUP] 2 3 [/SUP], Marco Binder[SUP] 4 [/SUP], Friedemann Weber[SUP] 5 6 [/SUP]
Affiliations
- PMID: 36637551
- DOI: 10.1007/s00705-022-05696-0
Abstract
Influenza A virus (FLUAV) is a significant human pathogen. In silico structural analysis (PMID 28628827) has suggested that the FDA-approved drug paliperidone interferes with the binding of the FLUAV polymerase subunit PB2 to the nucleoprotein NP. We found that paliperidone inhibits FLUAV A/PR/8/34 early after infection of canine MDCK II, human A549, and human primary bronchial cells, but not at late time points. No effect was detectable against the strains A/Hamburg/05/2009 and A/WSN/33. Moreover, paliperidone indeed disturbed the interaction between the PB2 and the NP of A/PR/8/34 and reduced early viral RNA and protein synthesis by approximately 50%. Thus, paliperidone has measurable but transient and virus-strain-restricted effects on FLUAV.