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Arch Virol. Analysis of a point mutation in H5N1 avian influenza virus hemagglutinin in relation to virus entry into live mammalian cells.

Giuseppe

Giuseppe

Emeritus
Arch Virol. 2008 Nov 20. [Epub ahead of print]

Analysis of a point mutation in H5N1 avian influenza virus hemagglutinin in relation to virus entry into live mammalian cells.

Su Y, Yang HY, Zhang BJ, Jia HL, Tien P. - Center for Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100080, People's Republic of China.

Binding to and infection of human cells is essential for avian influenza virus transmission.

Since virus binding is not always predictive for efficient infection of the cells, here we wished to investigate how hemagglutinin (HA) mutations of avian influenza virus H5N1 influence virus post-binding events in a single cycle of replication.

One mutation observed in H5 HA of avian and natural human isolates from mainland China, Hong Kong, Vietnam and Thailand was identified and analyzed.

The effects of the mutation on receptor binding, fusion and virus entry into cultured cells were investigated using hemadsorption, polykaryon formation and pseudotyped virus that express luciferase in the cytoplasm of transduced cell.

Our results revealed that replacing aspartic acid at residue 94 with asparagine enhanced virus fusion activity and increased the binding of HA to sialic acid alpha2,6 galactose, while it decreased pseudotyped virus entry into cells expressing the avian type receptor, sialic acid alpha2,3 galactose.

Our result may have implications for the understanding of the role of HA mutations in virus entry into live cells that exclusively display one type of receptor.
PMID: 19020946 [PubMed - as supplied by publisher
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Re: Arch Virol. Analysis of a point mutation in H5N1 avian influenza virus hemagglutinin in relation to virus entry into live mammalian cells.

what position
 
Re: Arch Virol. Analysis of a point mutation in H5N1 avian influenza virus hemagglutinin in relation to virus entry into live mammalian cells.

aspartic acid at residue 94 with asparagine
asp 94 asn, D 94 N
 
Re: Arch Virol. Analysis of a point mutation in H5N1 avian influenza virus hemagglutinin in relation to virus entry into live mammalian cells.

thanks. I had missed it.

94 is y

1 mekivllfai vslvksdqic igyhannste qvdtimeknv tvthaqdile kkhngklcdl
61 dgvkplilrd csvagwllgn pmcdefinvp ewsyivekan pvndlcypgd fndyeelkhl
121 lsrinhfeki qiipksswss heaslgvssa cpyqgkssff rnvvwlikkn styptikrsy
181 nntnqedllv lwgihhpnda aeqtklyqnp ttyisvgtst lnqrlvpria trskvngqsg
241 rmeffwtilk pndainfesn gnfiapeyay kivkkgdsti mkseleygnc ntkcqtpmga
301 inssmpfhni hpltigecpk yvksnrlvla tglrnspqre rrrkkrglfg aiagfieggw
361 qgmvdgwygy hhsneqgsgy aadkestqka idgvtnkvns iidkmntqfe avgrefnnle
421 rrienlnkkm edgfldvwty naellvlmen ertldfhdsn vknlydkvrl qlrdnakelg
481 ngcfefyhkc dnecmesvrn gtydypqyse earlkreeis gvklesigiy qilsiystva
541 sslalaimva glslwmcsng slqcrici


-----edit----------

no N in H5 at #94, neither #84. Maybe #110:
914 times D, 1040 times N,153 times S
 
Re: Arch Virol. Analysis of a point mutation in H5N1 avian influenza virus hemagglutinin in relation to virus entry into live mammalian cells.

Hi gsgs, wich strain do you screened ?
 
Re: Arch Virol. Analysis of a point mutation in H5N1 avian influenza virus hemagglutinin in relation to virus entry into live mammalian cells.

I speculate they mean: D199N

H5-sequences with 199N:

>A/Dk/HK/ww381/00(H5N1)
>A/Dk/HK/ww382/00(H5N1)
>A/Dk/HK/ww461/00(H5N1)
>A/Dk/HK/ww487/00(H5N1)
>A/Gs/HK/ww491/00(H5N1)
>A/Ck/El Salvador/102711-1/01(H5N2)
>A/Ck/El Salvador/102711-2/01(H5N2)
>A/Ck/HK/YU324/03(H5N1)
>A/Ck/YN/374/04(H5N1)
>A/IDN/CDC523/06(H5N1)
>A/IDN/CDC523E/06(H5N1)
>A/IDN/CDC523T/06(H5N1)
>A/IDN/CDC938/06(H5N1)
>A/IDN/CDC938E/06(H5N1)
>A/IDN/CDC887/06(H5N1)
>A/IDN/CDC1031/2007(H5N1)
>A/IDN/CDC1031T/2007(H5N1)
>A/IDN/CDC1031T2/2007(H5N1)
>A/IDN/CDC1031RE2/2007(H5N1)
>A/IDN/CDC1032/2007(H5N1)
>A/IDN/CDC1032N/2007(H5N1)
>A/IDN/CDC1032T/2007(H5N1)
>A/IDN/CDC1046/2007(H5N1)
>A/IDN/CDC1046T/2007(H5N1)
>A/IDN/CDC1047/2007(H5N1)
>A/IDN/CDC1047S/2007(H5N1)
>A/black bulbul/Fujian/439/04(H5N1)
>A/curlew/Shandong/61/04(H5N1)
>A/grey heron/HK/728/04(H5N1)
>A/grey heron/HK/837/04(H5N1)
>A/Chinese pond heron/HK/18/05(H5N1)
>A/China/GD01/06(H5N1)
>A/Guangzhou/1/06(H5N1)
>A/Dk/Guangxi/2926/05(H5N1)
>A/Gs/Guangxi/3017/05(H5N1)
>A/Dk/Guangxi/3085/05(H5N1)
>A/Ck/Guangxi/3154/05(H5N1)
>A/Dk/Guangxi/3364/05(H5N1)
>A/Dk/Guangxi/3548/05(H5N1)
>A/Gs/Guangxi/3714/05(H5N1)
>A/Dk/Guangxi/3741/05(H5N1)
>A/Ck/Guangxi/3791/05(H5N1)
>A/Dk/Guangxi/3819/05(H5N1)
>A/Dk/Guangxi/4184/05(H5N1)
>A/Dk/Guangxi/4196/05(H5N1)
>A/Dk/Guangxi/4665/05(H5N1)
>A/Dk/Guangxi/89/06(H5N1)
>A/Ck/Guiyang/2147/05(H5N1)
>A/Ck/Guiyang/2173/05(H5N1)
>A/Gs/Yunnan/3720/05(H5N1)
>A/Gs/Yunnan/4129/05(H5N1)
>A/Dk/Yunnan/4400/05(H5N1)
>A/Gs/Yunnan/4494/05(H5N1)
>A/Dk/Yunnan/4589/05(H5N1)
>A/Gs/Yunnan/4804/05(H5N1)
>A/Dk/Yunnan/5251/05(H5N1)
>A/Dk/Yunnan/5820/05(H5N1)
>A/Dk/Yunnan/5877/05(H5N1)
>A/Gs/Yunnan/6027/05(H5N1)
>A/Dk/Yunnan/6332/05(H5N1)
>A/Gs/Yunnan/6368/05(H5N1)
>A/Dk/Yunnan/6607/05(H5N1)
>A/Dk/Yunnan/1126/06(H5N1)
>A/Dk/Guangxi/4100/05(H5N1)
>A/Dk/Guangxi/4191/05(H5N1)
>A/Dk/Guangxi/4200/05(H5N1)
>A/Dk/Guangxi/4318/05(H5N1)
>A/Dk/Yunnan/4418/05(H5N1)
>A/Dk/Yunnan/4567/05(H5N1)
>A/Gs/Yunnan/4703/05(H5N1)
>A/Gs/Yunnan/4765/05(H5N1)
>A/Gs/Yunnan/4833/05(H5N1)
>A/Dk/Yunnan/5090/05(H5N1)
>A/Dk/Yunnan/5753/05(H5N1)
>A/Dk/Yunnan/5764/05(H5N1)
>A/Dk/Yunnan/5779/05(H5N1)
>A/Dk/Yunnan/5785/05(H5N1)
>A/Dk/Yunnan/5798/05(H5N1)
>A/Dk/Yunnan/5813/05(H5N1)
>A/Dk/Yunnan/5832/05(H5N1)
>A/Dk/Yunnan/5842/05(H5N1)
>A/Dk/Yunnan/5855/05(H5N1)
>A/Dk/Yunnan/5871/05(H5N1)
>A/Gs/Yunnan/6400/05(H5N1)
>A/Ck/Hunan/2246/06(H5N1)
>A/Ck/Hunan/2292/06(H5N1)
>A/Gs/Yunnan/2969/06(H5N1)
>A/Ck/Hunan/fg/04(H5N1)
>A/Dk/South Africa/811/04(H5N1)
>A/Qa/West Java/smijjg2/05(H5N1)
>A/Qa/West Java/Smigq1/05(H5N1)
>A/Gs/VNM/113/01(H5N1)
>A/Gs/VNM/324/01(H5N1)
>A/garganey/SanJiang/160/06(H5N2)
>A/Gs/Klapanunggal/IPB7-RS/06(H5N1)
>A/Dk/Parung/IPB8-RS/06(H5N1)
>A/Dk/Parung/IPB9-RS/06(H5N1)
>A/IDN/TLL012/06(H5N1)
>A/IDN/TLL014/06(H5N1)
>A/VNM/1203/04(H5N1)
>A/Ck/West Java/PWT-WIJ/06(H5N1)
>A/Ck/West Java/SMI-CSLK-EC/06(H5N1)
>A/Ck/West Java/SMI-PAT/06(H5N1)
>A/Ck/West Java/SMI-CSLK-EB/06(H5N1)
>A/Ck/Cicurug/IPB24-RS/06(H5N1)
 
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