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Appl Environ Microbiol. Persistence of Avian Influenza Virus (H5N1) in Feathers Detached from Bodies of Infected Domestic Ducks

Laidback Al

Well-known member
In 2006 several avian influenza human cases in Azerbaijan and Indonesia were suspected to have contracted H5N1 from poultry feathers. Swan feathers were implicated Azerbiajan. The 18 year old Indonesian was a shuttlecock maker.


Persistence of Avian Influenza Virus (H5N1) in Feathers Detached from Bodies of Infected Domestic Ducks


Applied and Environmental Microbiology, August 2010, p. 5496-5499, Vol. 76, No. 16 doi:10.1128/AEM.00563-10


Yu Yamamoto, Kikuyasu Nakamura, Manabu Yamada, and Masaji Mase <o:p></o:p>
National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki, Japan <o:p></o:p>
Received 3 March 2010/ Accepted 18 June 2010<o:p></o:p>

Asian lineage highly pathogenic avian influenza virus (H5N1)<sup> </sup>continues to cause mortality in poultry and wild bird populations<sup> </sup>at a panzootic scale. However, little is known about its persistence<sup> </sup>in contaminated tissues derived from infected birds. We investigated<sup> </sup>avian influenza virus (H5N1) persistence in feathers detached<sup> </sup>from bodies of infected ducks to evaluate their potential risk<sup> </sup>for environmental contamination. Four-week-old domestic ducks<sup> </sup>were inoculated with different clades of avian influenza virus<sup> </sup>(H5N1). Feathers, drinking water, and feces were collected on<sup> </sup>day 3 postinoculation and stored at 4?C or 20?C. Viral<sup> </sup>persistence in samples was investigated for 360 days by virus<sup> </sup>isolation and reverse transcription-PCR. Infectious viruses<sup> </sup>persisted for the longest period in feathers, compared with<sup> </sup>drinking water and feces, at both 4?C and 20?C. Viral<sup> </sup>infectivity persisted in the feathers for 160 days at 4?C<sup> </sup>and for 15 days at 20?C. Viral titers of 10<sup>4.3</sup> 50% egg infectious<sup> </sup>doses/ml or greater were detected for 120 days in feathers stored<sup> </sup>at 4?C. Viral RNA in feathers was more stable than the infectivity.<sup> </sup>These results indicate that feathers detached from domestic<sup> </sup>ducks infected with highly pathogenic avian influenza virus<sup> </sup>(H5N1) can be a source of environmental contamination and may<sup> </sup>function as fomites with high viral loads in the environment.

Also, see this thread:<o:p>
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http://www.flutrackers.com/forum/showpost.php?p=181776&postcount=1

hat tip to Carol@SC

<o:p></o:p>
 
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