tetano
Editor, Senior Moderator
Antiviral Res
. 2024 Mar 30:105877.
doi: 10.1016/j.antiviral.2024.105877. Online ahead of print. Supplementation of seasonal vaccine with multi-subtype neuraminidase and M2 ectodomain virus-like particle improves protection against homologous and heterologous influenza viruses in aged mice
Jannatul Ruhan Raha[SUP] 1 [/SUP], Ki-Hye Kim[SUP] 1 [/SUP], Noopur Bhatnagar[SUP] 1 [/SUP], Rong Liu[SUP] 1 [/SUP], Chau Thuy Tien Le[SUP] 1 [/SUP], Bo Ryoung Park[SUP] 1 [/SUP], Phillip Grovenstein[SUP] 1 [/SUP], Surya Sekhar Pal[SUP] 1 [/SUP], Eun-Ju Ko[SUP] 2 [/SUP], Chong Hyun Shin[SUP] 1 [/SUP], Bao-Zhong Wang[SUP] 1 [/SUP], Sang-Moo Kang[SUP] 3 [/SUP]
Affiliations
The conventional inactivated split seasonal influenza vaccine offers low efficacy, particularly in the elderly and against antigenic variants. Here, to improve the efficacy of seasonal vaccination for the elderly population, we tested whether supplementing seasonal bivalent (H1N1 + H3N2) split (S) vaccine with M2 ectodomain repeat and multi-subtype consensus neuraminidase (NA) proteins (N1 NA + N2 NA + flu B NA) on a virus-like particle (NA-M2e) would induce enhanced cross-protection against different influenza viruses in aged mice. Immunization with split vaccine plus NA-M2e (S + NA-M2e) increased vaccine-specific IgG antibodies towards T-helper type 1 responses and hemagglutination inhibition titers. Aged mice with NA-M2e supplemented vaccination were protected against homologous and heterologous viruses at higher efficacies, as evidenced by preventing weight loss, lowering lung viral loads, inducing broadly cross-protective humoral immunity, and IFN-γ[SUP]+[/SUP] CD4 and CD8 T cell responses than those with seasonal vaccine. Overall, this study supports a new strategy of NA-M2e supplemented vaccination to enhance protection against homologous and antigenically different viruses in the elderly.
Keywords: Aged mice; NA-M2e VLP; Split vaccine.
. 2024 Mar 30:105877.
doi: 10.1016/j.antiviral.2024.105877. Online ahead of print. Supplementation of seasonal vaccine with multi-subtype neuraminidase and M2 ectodomain virus-like particle improves protection against homologous and heterologous influenza viruses in aged mice
Jannatul Ruhan Raha[SUP] 1 [/SUP], Ki-Hye Kim[SUP] 1 [/SUP], Noopur Bhatnagar[SUP] 1 [/SUP], Rong Liu[SUP] 1 [/SUP], Chau Thuy Tien Le[SUP] 1 [/SUP], Bo Ryoung Park[SUP] 1 [/SUP], Phillip Grovenstein[SUP] 1 [/SUP], Surya Sekhar Pal[SUP] 1 [/SUP], Eun-Ju Ko[SUP] 2 [/SUP], Chong Hyun Shin[SUP] 1 [/SUP], Bao-Zhong Wang[SUP] 1 [/SUP], Sang-Moo Kang[SUP] 3 [/SUP]
Affiliations
- PMID: 38561077
- DOI: 10.1016/j.antiviral.2024.105877
The conventional inactivated split seasonal influenza vaccine offers low efficacy, particularly in the elderly and against antigenic variants. Here, to improve the efficacy of seasonal vaccination for the elderly population, we tested whether supplementing seasonal bivalent (H1N1 + H3N2) split (S) vaccine with M2 ectodomain repeat and multi-subtype consensus neuraminidase (NA) proteins (N1 NA + N2 NA + flu B NA) on a virus-like particle (NA-M2e) would induce enhanced cross-protection against different influenza viruses in aged mice. Immunization with split vaccine plus NA-M2e (S + NA-M2e) increased vaccine-specific IgG antibodies towards T-helper type 1 responses and hemagglutination inhibition titers. Aged mice with NA-M2e supplemented vaccination were protected against homologous and heterologous viruses at higher efficacies, as evidenced by preventing weight loss, lowering lung viral loads, inducing broadly cross-protective humoral immunity, and IFN-γ[SUP]+[/SUP] CD4 and CD8 T cell responses than those with seasonal vaccine. Overall, this study supports a new strategy of NA-M2e supplemented vaccination to enhance protection against homologous and antigenically different viruses in the elderly.
Keywords: Aged mice; NA-M2e VLP; Split vaccine.