Giuseppe
Emeritus
[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]
Antiviral Res. 2014 Apr 15. pii: S0166-3542(14)00109-0. doi: 10.1016/j.antiviral.2014.04.003. [Epub ahead of print]
Induction of neutralizing antibodies to influenza A virus H7N9 by inactivated whole virus in mice and nonhuman primates.
Pan W<SUP>1</SUP>, Han L<SUP>2</SUP>, Dong Z<SUP>3</SUP>, Niu X<SUP>1</SUP>, Li Z<SUP>3</SUP>, Bao L<SUP>4</SUP>, Li C<SUP>1</SUP>, Luo Q<SUP>1</SUP>, Yang Z<SUP>1</SUP>, Li X<SUP>4</SUP>, Huang J<SUP>5</SUP>, Feng L<SUP>3</SUP>, Qin C<SUP>4</SUP>, Zhong N<SUP>1</SUP>, Chen L<SUP>6</SUP>.
Author information: <SUP>1</SUP>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. <SUP>2</SUP>Anhui University, Anhui, China. <SUP>3</SUP>Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences, Guangzhou, China. <SUP>4</SUP>Institute of Laboratory Sciences (ILS), Chinese Academy of Medical Sciences, Beijing, China. <SUP>5</SUP>Health Quarantine Laboratory, Guangdong Inspection and Quarantine Technology Center,Guangzhou, China. <SUP>6</SUP>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China; Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences, Guangzhou, China. Electronic address: hen002@gmail.com.
Abstract
We evaluated the immunogenicity of hemagglutinin (HA) in the context of inactivated H7N9/AH/1/13-PR8 whole-virion. At four weeks after immunization with 15μg HA, mice produced hemagglutination inhibition (HI) titers of 1:192 and neutralizing antibodies of 1:317. Aluminum hydroxide (alum), or a booster immunization, or both increased HI to 1:768, 1:384, 1:896 and neutralizing antibodies to 1:1868, 1:2302, 1:10,000 respectively. Macaques generated HI of 1:190 or 1:360 and virus neutralizing titers of 1:280 or 1:658 at three weeks after immunization with HA alone or with alum. Sera from immunized mice and macaques protected mice from infection of A/Anhui/1/2013 (H7N9), suggesting an H7N9 vaccine is immunologically feasible.
Copyright ? 2014. Published by Elsevier B.V.
KEYWORDS: Antibody, H7N9, Hemagglutinin, Influenza, Neutralization
PMID: 24746458 [PubMed - as supplied by publisher]
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Antiviral Res. 2014 Apr 15. pii: S0166-3542(14)00109-0. doi: 10.1016/j.antiviral.2014.04.003. [Epub ahead of print]
Induction of neutralizing antibodies to influenza A virus H7N9 by inactivated whole virus in mice and nonhuman primates.
Pan W<SUP>1</SUP>, Han L<SUP>2</SUP>, Dong Z<SUP>3</SUP>, Niu X<SUP>1</SUP>, Li Z<SUP>3</SUP>, Bao L<SUP>4</SUP>, Li C<SUP>1</SUP>, Luo Q<SUP>1</SUP>, Yang Z<SUP>1</SUP>, Li X<SUP>4</SUP>, Huang J<SUP>5</SUP>, Feng L<SUP>3</SUP>, Qin C<SUP>4</SUP>, Zhong N<SUP>1</SUP>, Chen L<SUP>6</SUP>.
Author information: <SUP>1</SUP>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. <SUP>2</SUP>Anhui University, Anhui, China. <SUP>3</SUP>Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences, Guangzhou, China. <SUP>4</SUP>Institute of Laboratory Sciences (ILS), Chinese Academy of Medical Sciences, Beijing, China. <SUP>5</SUP>Health Quarantine Laboratory, Guangdong Inspection and Quarantine Technology Center,Guangzhou, China. <SUP>6</SUP>State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China; Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences, Guangzhou, China. Electronic address: hen002@gmail.com.
Abstract
We evaluated the immunogenicity of hemagglutinin (HA) in the context of inactivated H7N9/AH/1/13-PR8 whole-virion. At four weeks after immunization with 15μg HA, mice produced hemagglutination inhibition (HI) titers of 1:192 and neutralizing antibodies of 1:317. Aluminum hydroxide (alum), or a booster immunization, or both increased HI to 1:768, 1:384, 1:896 and neutralizing antibodies to 1:1868, 1:2302, 1:10,000 respectively. Macaques generated HI of 1:190 or 1:360 and virus neutralizing titers of 1:280 or 1:658 at three weeks after immunization with HA alone or with alum. Sera from immunized mice and macaques protected mice from infection of A/Anhui/1/2013 (H7N9), suggesting an H7N9 vaccine is immunologically feasible.
Copyright ? 2014. Published by Elsevier B.V.
KEYWORDS: Antibody, H7N9, Hemagglutinin, Influenza, Neutralization
PMID: 24746458 [PubMed - as supplied by publisher]
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