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J Microbiol Biotechnol. 2019 Nov 18. doi: 10.4014/jmb.1908.08001. [Epub ahead of print] [h=1]Antiviral Activity of Fritillaria thunbergii extract against Human Influenza Virus H1N1 (PR8) in vitro, in ovo and in vivo.[/h]
Kim M[SUP]1[/SUP], Nguyen DV[SUP]1[/SUP], Heo Y[SUP]1[/SUP], Park KH[SUP]1[/SUP], Paik HD[SUP]2[/SUP], Kim YB[SUP]1[/SUP].
[h=3]Author information[/h] 1 Department of Biomedical Science and Engineering, Konkuk University, Seoul, 05029, Republic of Korea. 2 Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea.
[h=3]Abstract[/h] .Influenza viruses cause respiratory diseases in humans and animals with high morbidity and mortality rates. Conventional anti-influenza drugs are reported to exert side-effects and newly emerging viral strains tend to develop resistance to these commonly used agents. Fritillaria thunbergii (FT) is traditionally used as an expectorant for controlling airway inflammatory disorders. Here, we evaluated the therapeutic effects of FT extracts against influenza virus type A (H1N1) infection in vitro, in ovo, and in vivo. In the post-treatment assay, FT extracts showed high CC50 (7500 ?g/ml) indicating low toxicity and exerted moderate antiviral effects, compared to Oseltamivir (SI 50.6 vs. 222) in vitro. Antiviral activity tests in ovo revealed strong inhibitory effects of both FT extract and Oseltamivir against H1N1 replication in embryonated eggs. Notably, at a treatment concentration of 150 mg/kg, only half the group administered Oseltamivir survived whereas the FT group showed 100% survival, clearly supporting low toxicity of FT extracts. Consistent with these findings, FT-administered mice showed a higher survival rate with lower body weight reduction relative to the Oseltamivir group upon treatment 24 h after viral infection. Our collective results suggest that FT extracts exert antiviral effects against influenza H1N1 virus without inducing toxicity in vitro, in ovo and in vivo, supporting its potential utility as a novel candidate therapeutic drug or supplement against influenza.
[h=4]KEYWORDS:[/h] Antiviral activity; Fritillaria thunbergii; Influenza A (H1N1); Oseltamivir
PMID: 31752057 DOI: 10.4014/jmb.1908.08001
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Kim M[SUP]1[/SUP], Nguyen DV[SUP]1[/SUP], Heo Y[SUP]1[/SUP], Park KH[SUP]1[/SUP], Paik HD[SUP]2[/SUP], Kim YB[SUP]1[/SUP].
[h=3]Author information[/h] 1 Department of Biomedical Science and Engineering, Konkuk University, Seoul, 05029, Republic of Korea. 2 Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea.
[h=3]Abstract[/h] .Influenza viruses cause respiratory diseases in humans and animals with high morbidity and mortality rates. Conventional anti-influenza drugs are reported to exert side-effects and newly emerging viral strains tend to develop resistance to these commonly used agents. Fritillaria thunbergii (FT) is traditionally used as an expectorant for controlling airway inflammatory disorders. Here, we evaluated the therapeutic effects of FT extracts against influenza virus type A (H1N1) infection in vitro, in ovo, and in vivo. In the post-treatment assay, FT extracts showed high CC50 (7500 ?g/ml) indicating low toxicity and exerted moderate antiviral effects, compared to Oseltamivir (SI 50.6 vs. 222) in vitro. Antiviral activity tests in ovo revealed strong inhibitory effects of both FT extract and Oseltamivir against H1N1 replication in embryonated eggs. Notably, at a treatment concentration of 150 mg/kg, only half the group administered Oseltamivir survived whereas the FT group showed 100% survival, clearly supporting low toxicity of FT extracts. Consistent with these findings, FT-administered mice showed a higher survival rate with lower body weight reduction relative to the Oseltamivir group upon treatment 24 h after viral infection. Our collective results suggest that FT extracts exert antiviral effects against influenza H1N1 virus without inducing toxicity in vitro, in ovo and in vivo, supporting its potential utility as a novel candidate therapeutic drug or supplement against influenza.
[h=4]KEYWORDS:[/h] Antiviral activity; Fritillaria thunbergii; Influenza A (H1N1); Oseltamivir
PMID: 31752057 DOI: 10.4014/jmb.1908.08001
Free full text