Giuseppe
Emeritus
[Source: Antimicrobial Agents and Chemotherapy, full page: (LINK). Abstract, edited.]
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Rectal swabs are suitable for quantifying the carriage load of KPC-producing carbapenem-resistant Enterobacteriaceae (CRE)
A. Lerner a, J. Romano a,b, I. Chmelnitsky a, S. Navon-Venezia a, R. Edgar a and Y. Carmeli a,#
Author Affiliations: <SUP>a</SUP>Molecular Epidemiology and Antimicrobial Resistance Laboratory, Division of Epidemiology, Tel Aviv Sourasky Medical Center, Affiliated to Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel <SUP>b</SUP>GluSense LTD, 7 Lev Pesach Street, Lod 71293, Israel
ABSTRACT
It is more convenient and practical to collect rectal-swabs than stool specimens for studying carriage of colon pathogens. Herein we examined the ability to use rectal-swabs rather than stool specimens to quantify KPC-producing carbapenem-resistant Enterobacteriaceae (CRE). We used a qPCR assay to determine the concentration of bla<SUB>KPC</SUB> relative to 16S rDNA genes, and a quantitative culture-based method to quantify CRE relative to total aerobic bacteria. Our results demonstrated that rectal-swabs are suitable for quantifying the concentration of KPC-producing CRE and that qPCR showed higher correlation between rectal-swabs and stool compared to the culture-based method.
FOOTNOTES
#Correspondence to: Molecular Epidemiology and Antimicrobial Resistance Laboratory, Division of Epidemiology, Tel Aviv Sourasky Medical Center, 6 Weizmann Street, Tel Aviv 64239, Israel. yehudac@tasmc.health.gov.il, 972-3-6973388.
Copyright ? 2013, American Society for Microbiology. All Rights Reserved.
-A. Lerner a, J. Romano a,b, I. Chmelnitsky a, S. Navon-Venezia a, R. Edgar a and Y. Carmeli a,#
Author Affiliations: <SUP>a</SUP>Molecular Epidemiology and Antimicrobial Resistance Laboratory, Division of Epidemiology, Tel Aviv Sourasky Medical Center, Affiliated to Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel <SUP>b</SUP>GluSense LTD, 7 Lev Pesach Street, Lod 71293, Israel
ABSTRACT
It is more convenient and practical to collect rectal-swabs than stool specimens for studying carriage of colon pathogens. Herein we examined the ability to use rectal-swabs rather than stool specimens to quantify KPC-producing carbapenem-resistant Enterobacteriaceae (CRE). We used a qPCR assay to determine the concentration of bla<SUB>KPC</SUB> relative to 16S rDNA genes, and a quantitative culture-based method to quantify CRE relative to total aerobic bacteria. Our results demonstrated that rectal-swabs are suitable for quantifying the concentration of KPC-producing CRE and that qPCR showed higher correlation between rectal-swabs and stool compared to the culture-based method.
FOOTNOTES
#Correspondence to: Molecular Epidemiology and Antimicrobial Resistance Laboratory, Division of Epidemiology, Tel Aviv Sourasky Medical Center, 6 Weizmann Street, Tel Aviv 64239, Israel. yehudac@tasmc.health.gov.il, 972-3-6973388.
Copyright ? 2013, American Society for Microbiology. All Rights Reserved.
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