tetano
Editor, Senior Moderator
Microbiol Immunol. 2017 Mar 30. doi: 10.1111/1348-0421.12478. [Epub ahead of print]
[h=1]Antigenic diversity of H5 highly pathogenic avian influenza viruses of clade 2.3.4.4 isolated in Asia.[/h] Ohkawara A[SUP]1[/SUP], Okamatsu M[SUP]1[/SUP], Ozawa M[SUP]2,[/SUP][SUP]3,[/SUP][SUP]4[/SUP], Chu DH[SUP]1[/SUP], Nguyen LT[SUP]1[/SUP], Hiono T[SUP]1[/SUP], Matsuno K[SUP]1,[/SUP][SUP]5[/SUP], Kida H[SUP]5,[/SUP][SUP]6[/SUP], Sakoda Y[SUP]1,[/SUP][SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] H5 highly pathogenic avian influenza viruses (HPAIVs) have spread in both poultry and wild birds since the late 2003. The continued circulation of HPAIVs in poultry in several regions of the world has led to antigenic drift. In this study, we analyzed the antigenic properties of H5 HPAIVs isolated in Asia using four neutralizing monoclonal antibodies (MAbs) recognizing the hemagglutinin, which were established using A/chicken/Kumamoto/1-7/2014 (H5N8), belonging to clade 2.3.4.4 and also using polyclonal antibodies. Viruses of clades 1.1, 2.3.2.1, 2.3.4, and 2.3.4.4 had different reactivity patterns to the panel of MAbs, thereby indicating that the antigenicity of the viruses of clade 2.3.4.4 were similar but differed from other clades. In particular, the antigenicity of the viruses of clade 2.3.4.4 differed from those of the viruses of clades 2.3.4 and 2.3.2.1, which suggests that the recent H5 HPAIVs have further evolved antigenically divergent. In addition, reactivity of antiserum suggest that the antigenicity of viruses of clade 2.3.4.4 differed slightly among group A, B, and C. Vaccines are still used in poultry in the endemic countries, so the antigenicity of H5 HPAIVs should be monitored continually to facilitate the control of avian influenza. The panel of MAbs established in the present study will be useful for detecting antigenic drift in the H5 viruses that emerge from the current strains.
? 2017 The Societies and Wiley Publishing Asia Pty Ltd.
[h=4]KEYWORDS:[/h] H5N8; antigenicity; hemagglutinin; highly pathogenic avian influenza virus
PMID: 28370432 DOI: 10.1111/1348-0421.12478
[h=1]Antigenic diversity of H5 highly pathogenic avian influenza viruses of clade 2.3.4.4 isolated in Asia.[/h] Ohkawara A[SUP]1[/SUP], Okamatsu M[SUP]1[/SUP], Ozawa M[SUP]2,[/SUP][SUP]3,[/SUP][SUP]4[/SUP], Chu DH[SUP]1[/SUP], Nguyen LT[SUP]1[/SUP], Hiono T[SUP]1[/SUP], Matsuno K[SUP]1,[/SUP][SUP]5[/SUP], Kida H[SUP]5,[/SUP][SUP]6[/SUP], Sakoda Y[SUP]1,[/SUP][SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] H5 highly pathogenic avian influenza viruses (HPAIVs) have spread in both poultry and wild birds since the late 2003. The continued circulation of HPAIVs in poultry in several regions of the world has led to antigenic drift. In this study, we analyzed the antigenic properties of H5 HPAIVs isolated in Asia using four neutralizing monoclonal antibodies (MAbs) recognizing the hemagglutinin, which were established using A/chicken/Kumamoto/1-7/2014 (H5N8), belonging to clade 2.3.4.4 and also using polyclonal antibodies. Viruses of clades 1.1, 2.3.2.1, 2.3.4, and 2.3.4.4 had different reactivity patterns to the panel of MAbs, thereby indicating that the antigenicity of the viruses of clade 2.3.4.4 were similar but differed from other clades. In particular, the antigenicity of the viruses of clade 2.3.4.4 differed from those of the viruses of clades 2.3.4 and 2.3.2.1, which suggests that the recent H5 HPAIVs have further evolved antigenically divergent. In addition, reactivity of antiserum suggest that the antigenicity of viruses of clade 2.3.4.4 differed slightly among group A, B, and C. Vaccines are still used in poultry in the endemic countries, so the antigenicity of H5 HPAIVs should be monitored continually to facilitate the control of avian influenza. The panel of MAbs established in the present study will be useful for detecting antigenic drift in the H5 viruses that emerge from the current strains.
? 2017 The Societies and Wiley Publishing Asia Pty Ltd.
[h=4]KEYWORDS:[/h] H5N8; antigenicity; hemagglutinin; highly pathogenic avian influenza virus
PMID: 28370432 DOI: 10.1111/1348-0421.12478