tetano
Editor, Senior Moderator
Vet Microbiol. 2013 Jun 20. pii: S0378-1135(13)00313-1. doi: 10.1016/j.vetmic.2013.05.029. [Epub ahead of print]
Antibody survey on avian influenza viruses using egg yolks of ducks in Hanoi between 2010 and 2012.
Hotta K, Takakuwa H, Yabuta T, Ung TT, Usui T, Nguyen HL, Le TT, Le MQ, Yamaguchi T, Otsuki K, Ito T, Murase T, Yamashiro T.
Source
Center for Infectious Disease Research in Asia and Africa, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan; Vietnam Research Station, Nagasaki University, c/o National Institute of Hygiene and Epidemiology, No. 1 Yersin Street, Hanoi, Viet Nam. Electronic address: kozue@nagasaki-u.ac.jp.
Abstract
In Vietnam, numerous surveillance programs are conducted to monitor the prevalence of avian influenza (AI) viruses. Three serological methods-the agar-gel immunodiffusion test, hemagglutination inhibition (HI) test, and enzyme-linked immunosorbent assay-are well established for detection of AI virus antibodies in poultry sera. Several recent reports have validated egg yolk as an alternative source for detection of AI virus antibodies. In this study, we investigated AI virus antibodies in ducks by HI testing using egg yolk. Ten duck eggs were collected every month from 10 randomly selected markets in Hanoi from April 2010 to March 2012. The HI test was performed using low pathogenic avian influenza (LPAI) viruses (H3, H4, H6, H7, H9, and H11 subtypes) and highly pathogenic avian influenza (HPAI) viruses (H5N1 clade 2.3.4 and 2.3.2.1) as antigens. HI testing for H3, H6, and H9 was 29% positive in November 2010, 50% positive in October and November 2010, and 12% positive in June 2011. These results indicated that several epidemics of LPAI viruses had occurred during the study period. In addition, antibodies against H7 were negative. The results of HI testing for H5N1 showed that the reactivity of the dominant HI antibody shifted from H5N1 clade 2.3.4 to clade 2.3.2.1. In conclusion, egg yolk is useful for long term monitoring of AI virus antibodies and the use of egg-based antibody detection may contribute to improvements in animal welfare.
Copyright ? 2013 Elsevier B.V. All rights reserved.
KEYWORDS:
Avian influenza virus, Egg yolk, Hemagglutination inhibition test
PMID:
23849095
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23849095
Antibody survey on avian influenza viruses using egg yolks of ducks in Hanoi between 2010 and 2012.
Hotta K, Takakuwa H, Yabuta T, Ung TT, Usui T, Nguyen HL, Le TT, Le MQ, Yamaguchi T, Otsuki K, Ito T, Murase T, Yamashiro T.
Source
Center for Infectious Disease Research in Asia and Africa, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan; Vietnam Research Station, Nagasaki University, c/o National Institute of Hygiene and Epidemiology, No. 1 Yersin Street, Hanoi, Viet Nam. Electronic address: kozue@nagasaki-u.ac.jp.
Abstract
In Vietnam, numerous surveillance programs are conducted to monitor the prevalence of avian influenza (AI) viruses. Three serological methods-the agar-gel immunodiffusion test, hemagglutination inhibition (HI) test, and enzyme-linked immunosorbent assay-are well established for detection of AI virus antibodies in poultry sera. Several recent reports have validated egg yolk as an alternative source for detection of AI virus antibodies. In this study, we investigated AI virus antibodies in ducks by HI testing using egg yolk. Ten duck eggs were collected every month from 10 randomly selected markets in Hanoi from April 2010 to March 2012. The HI test was performed using low pathogenic avian influenza (LPAI) viruses (H3, H4, H6, H7, H9, and H11 subtypes) and highly pathogenic avian influenza (HPAI) viruses (H5N1 clade 2.3.4 and 2.3.2.1) as antigens. HI testing for H3, H6, and H9 was 29% positive in November 2010, 50% positive in October and November 2010, and 12% positive in June 2011. These results indicated that several epidemics of LPAI viruses had occurred during the study period. In addition, antibodies against H7 were negative. The results of HI testing for H5N1 showed that the reactivity of the dominant HI antibody shifted from H5N1 clade 2.3.4 to clade 2.3.2.1. In conclusion, egg yolk is useful for long term monitoring of AI virus antibodies and the use of egg-based antibody detection may contribute to improvements in animal welfare.
Copyright ? 2013 Elsevier B.V. All rights reserved.
KEYWORDS:
Avian influenza virus, Egg yolk, Hemagglutination inhibition test
PMID:
23849095
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23849095