tetano
Editor, Senior Moderator
Methods Mol Biol. 2014;1161:347-53. doi: 10.1007/978-1-4939-0758-8_29.
Antibody secreting cell assay for influenza a virus in Swine.
Kitikoon P1, Loving CL, Vincent AL.
Author information
Abstract
An ELISPOT assay to enumerate B-cells producing antibodies specific to a given antigen, also known as an antibody secreting cell (ASC) assay, was adapted to detect B-cells specific for influenza A virus (IAV). The assay is performed ex vivo and enumerates ASC at a single cell level. A simple ASC detection method is based on a solid phase immunoenzymatic principle. The ELISPOT plate is coated with IAV prior to incubation with cell samples. The spot-forming cells are detected by enzyme labeled antibodies directed to the swine immunoglobulin (Ig) class of interest and visualized with the addition of substrate.
PMID:
24899443
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24899443
Antibody secreting cell assay for influenza a virus in Swine.
Kitikoon P1, Loving CL, Vincent AL.
Author information
Abstract
An ELISPOT assay to enumerate B-cells producing antibodies specific to a given antigen, also known as an antibody secreting cell (ASC) assay, was adapted to detect B-cells specific for influenza A virus (IAV). The assay is performed ex vivo and enumerates ASC at a single cell level. A simple ASC detection method is based on a solid phase immunoenzymatic principle. The ELISPOT plate is coated with IAV prior to incubation with cell samples. The spot-forming cells are detected by enzyme labeled antibodies directed to the swine immunoglobulin (Ig) class of interest and visualized with the addition of substrate.
PMID:
24899443
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24899443